Empower 2 tm advanced software

Amino acid (AA) profiles were determined according to the procedure described by Vargas-Ramella et al. [23] . The HPLC system used was an Alliance 2695 model equipped with a 2475 scanning fluorescence detector (Waters, Milford, MA, USA). To control the system operation and results management, an Empower 2 TM advanced software (Version 1.04.1037; Waters, Milford, MA, USA) was used. Separations were carried out using a Waters AccQ-Tag column (3.9 × 150 mm, with a 4 mm particle size) with a flow rate of 1.0 mL min-1 at 37 • C. Detection was carried out via fluorescence with excitation at 250 nm and emission at 395 nm. The AAs were quantified using the external standard method using an AA standard (Amino Acid Standard H, Thermo, Rockford, IL, USA) and identified via the retention time. The amount of each AA that was detected was expressed as mg/100 g sample.