Eif2ak2

Total RNA purification was performed using High Pure RNA Isolation Kit (Roche Life Science) with an on‐column enzymatic DNA degradation, according to the manufacturer's instructions. RNA concentration was determined on a Nanodrop spectrophotometer and cDNA created using equal amounts of RNA and the TaqMan^® Reverse Transcription Reagents with random primers according to manufacturer's instructions (Applied Biosystems). Quantitative PCR was performed using the iTaq^™ SYBR^® Green Mastermix with ROX (BioRad) with the following probes: NADH (NDUFC2, Hs01072843_m1), tau (MAPT, Hs00902194_m1), PKR (EIF2AK2, Hs00169345_m1), β‐actin (ActB, Mm00607939_s1), IFN‐β (Ifnb1, Mm00439552_s1), TNF‐α (Tnf, Mm00443260_g1), tau (MAPT, Mm00521988_m1), neuron‐specific class III beta‐tubulin (Tubb3, Mm00727586_s1) and PKR (EIF2AK2, Mm01235643_m1, all Thermo Fisher Scientific Inc.).