G401 cells

G401 cells (ATCC) were cultured in RPMI-1640 (Gibco) media with 10% fetal bovine serum (FBS) (Hyclone), penicillin (10,000 I.U./mL), streptomycin (10,000 μg/mL), and L-glutamine (2 mM). Cells were grown in log phase by maintaining the population at a concentration of 500,000 cells per mL. HEK293T (ATCC) cells were cultured in DMEM, 10% fetal bovine serum (FBS), penicillin (10,000 I.U./mL) and streptomycin (10,000 μg/mL). G401 and HEK293T cells were maintained in a controlled humidified incubator at 37°C, with 5% CO2.

K562 cells (ATCC CCL-243) were cultured in Iscove’s Modified Dulbecco’s Medium supplemented with 10% FBS and antibiotics. T47D cells (ATCC HTB-133), NCI-H460 cells (ATCC HTB-177), A549 cells (ATCC CCL-185), LNCaP (ATCC CRL-1740), and GM12878 cells (Coriell) were cultured in RPMI-1640 supplemented with 10% FBS and antibiotics, or 15% FBS and antibiotics (GM12878). Caki2 cells (ATCC HTB-47), G-401 cells (ATCC CRL-1441) were cultured in McCoy’s 5a Medium Modified supplemented with 10% FBS and antibiotics. PANC-1 cells (ATCC CRL-1469) were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% FBS and antibiotics. SK-N-MC (ATCC HTB-10), RPMI-7951 (ATCC HTB-66) cells were cultured in Eagle’s Minimum Essential Medium supplemented with 10% FBS and antibiotics. SK-N-AS cells (ATCC CRL-2137) were cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% FBS, 0.1mM Non-Essential Amino Acids (Gibco) and antibiotics All cell lines cultured as part of ENCODE data generation (A549, Caki2, G401, LNCaP, NCI-H460, Panc1, RPMI-7951, SJCRH30, SK-MEL-5, SK-N-DZ, SK-N-MC,T47D) were cultured using standardized protocols, the details of which can be found through the ENCODE consortium website (https://www.encodeproject.org/).