For primary AML samples, cells were stained with anti-human CD45 conjugated to allophycocyanin-cyanine (APC-H7; 2D1, BD Biosciences, Franklin Lakes, NJ, USA), CD34 conjugated to phycoerythrin-cyanine-7 (PECy7; 581, BD Biosciences, Franklin Lakes, NJ, USA ) and CD38 conjugated to allophycocyanin (APC; HB7, BD Biosciences, Franklin Lakes, NJ, USA ) and Ferroportin (FPN) conjugated to Alexa Fluor-405 (AF-405; 8G10NB, Novus Biologicals, Littleton, CO, USA) or isotype control AF-405 (mouse IgG2b; MPC11 (Novus Biologicals, Littleton, CO, USA )) for 30 min in the dark at 4oC. For cell lines, cells were only stained with FPN-AF-405 or Isotype control for 30 min in the dark at 4oC. Cells were washed two times and resuspended in cold FACS buffer containing a viability dye (7-aminoactinomycin; 7AAD, Life Technologies, Carlsbad, CA, USA). Cells were evaluated in a BD-LSRII analytical flow cytometer. Analysis was later performed using FlowJo 9.3 software for Mac OS X (TreeStar, Ashland, OR).
Mouse igg2b mpc11
Manufactured by Novus Biologicals
Sourced in United States
Mouse IgG2b; MPC11 is a laboratory reagent that can be used for research purposes. It is a monoclonal antibody derived from the mouse hybridoma cell line MPC11.
Automatically generated - may contain errors
2 protocols using mouse igg2b mpc11
1
Ferroportin Expression in Primary AML
2
Ferroportin Expression in Primary AML
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For primary AML samples, cells were stained with anti-human CD45 conjugated to allophycocyanin-cyanine (APC-H7; 2D1, BD Biosciences, Franklin Lakes, NJ, USA), CD34 conjugated to phycoerythrin-cyanine-7 (PECy7; 581, BD Biosciences, Franklin Lakes, NJ, USA ) and CD38 conjugated to allophycocyanin (APC; HB7, BD Biosciences, Franklin Lakes, NJ, USA ) and Ferroportin (FPN) conjugated to Alexa Fluor-405 (AF-405; 8G10NB, Novus Biologicals, Littleton, CO, USA) or isotype control AF-405 (mouse IgG2b; MPC11 (Novus Biologicals, Littleton, CO, USA )) for 30 min in the dark at 4oC. For cell lines, cells were only stained with FPN-AF-405 or Isotype control for 30 min in the dark at 4oC. Cells were washed two times and resuspended in cold FACS buffer containing a viability dye (7-aminoactinomycin; 7AAD, Life Technologies, Carlsbad, CA, USA). Cells were evaluated in a BD-LSRII analytical flow cytometer. Analysis was later performed using FlowJo 9.3 software for Mac OS X (TreeStar, Ashland, OR).
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