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Orca flash 4.0 lt cmos digital camera

Manufactured by Hamamatsu Photonics
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Sourced in Japan

The Orca-Flash 4.0 LT CMOS Digital Camera is a high-performance imaging device manufactured by Hamamatsu Photonics. It features a CMOS image sensor with a resolution of 4.2 megapixels and a maximum frame rate of 100 frames per second. The camera is designed for various applications that require reliable and efficient image capture.

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Lab products found in correlation

Na 1.1 ld c apochromatic water immersion objective, by Hamamatsu Photonics (2 mentions) Axio observer z1 7, by Zeiss (2 mentions) Glass bottomed dishes, by World Precision Instruments (1 mentions) Poly d lysine, by Merck Group (1 mentions) Axio observer z, by Zeiss (1 mentions)

Close spelling variants of this product

ORCA Flash 4.0 CMOS Orca-FLASH 4.0 digital Flash 4.0 LT Orca 4.0 camera Flash 4.0 camera Orca Flash 4.0 CMOS camera Flash 4.0 Digital camera

4 protocols using orca flash 4.0 lt cmos digital camera

1

Microscopic Imaging of Marine Cells

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Glass-bottomed dishes (World Precision Instruments, Sarasota, FL; Cat. No. FD35-100) were prepared for imaging by covering the bottom with 500 µl of 0.1 mg/ml poly-D-lysine (Millipore Sigma; Cat. No. P6407-5MG) and incubating for 15 min. The poly-D-lysine was removed and then the dish was washed three times with 500 µl of ASW. Cells were placed into the dish by gently pipetting 500 µl of cells with a wide pipette tip.
Differential interference contrast (DIC) microscopy images were captured with a Zeiss Axio Observer.Z1/7 Widefield microscope with a Hamamatsu Orca-Flash 4.0 LT CMOS Digital Camera (Hamamatsu Photonics, Hamamatsu City, Japan) and 40×/NA 1.1 LD C-Apochromatic water immersion, 63×/NA1.40 Plan-Apochromatic oil immersion, or 100 × NA 1.40 Plan-Apochromatic oil immersion objectives (Zeiss).
Booth D.S, & King N. (2020). Genome editing enables reverse genetics of multicellular development in the choanoflagellate Salpingoeca rosetta. eLife, 9, e56193.
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2

Measuring Cilium Length in Cells

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To measure cilium length, cells grown to mid-log phase were fixed and stained using 1 part Lugol’s solution (EMD Millipore Cat. No. 1.09261.1000) with 3 parts culture (usually 25 μl and 75 μl). 4 μl were loaded onto a slide, spread by placing a No. 1.5 coverslip on thee sample, and imaged coverslip slide down with a Zeiss Axio Observer.Z1/7 Widefield microscope with a Hamamatsu Orca-Flash 4.0 LT CMOS Digital Camera (Hamamatsu Photonics, Hamamatsu City, Japan) and 40×/NA 1.1 LD C-Apochromatic water immersion objective. Images were acquired with 10 ms exposure and 8.0 V of light intensity, using the PH3 phase contrast ring. Ciliary lengths were traced and measured in Fiji94 (link).
Coyle M.C., Tajima A.M., Leon F., Choksi S.P., Yang A., Espinoza S., Hughes T.R., Reiter J.F., Booth D.S, & King N. (2023). An RFX transcription factor regulates ciliogenesis in the closest living relatives of animals. Current biology : CB, 33(17), 3747-3758.e9.
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3

Measuring Cilium Length in Cells

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To measure cilium length, cells grown to mid-log phase were fixed and stained using 1 part Lugol’s solution (EMD Millipore Cat. No. 1.09261.1000) with 3 parts culture (usually 25 μl and 75 μl). 4 μl were loaded onto a slide, spread by placing a No. 1.5 coverslip on thee sample, and imaged coverslip slide down with a Zeiss Axio Observer.Z1/7 Widefield microscope with a Hamamatsu Orca-Flash 4.0 LT CMOS Digital Camera (Hamamatsu Photonics, Hamamatsu City, Japan) and 40×/NA 1.1 LD C-Apochromatic water immersion objective. Images were acquired with 10 ms exposure and 8.0 V of light intensity, using the PH3 phase contrast ring. Ciliary lengths were traced and measured in Fiji94 (link).
Lu M, & Kosik K.S. (2001). Competition for Microtubule-binding with Dual Expression of Tau Missense and Splice Isoforms. Molecular Biology of the Cell, 12(1), 171-184.
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4

Quantification of Choanoflagellate Cell Clusters

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Cell cluster areas were quantified as described in reference 18 (link). Briefly, samples were imaged in 96-well glass-bottomed plates (Ibidi 89621) at ×10 magnification using transmitted light (bright field) on a Zeiss Axio Observer.Z1/7 widefield microscope with a Hamamatsu Orca-Flash 4.0 LT CMOS digital camera. Images from 3 biological replicates were processed and analyzed with the following functions in ImageJ: “smooth” to reduce bacterial background, “find edges” to further highlight choanoflagellate cells, “make binary” to convert to black and white, “close-” to fill in small holes, and “analyze particles” to calculate the area of each cell cluster. Particles smaller than 10 μm2 were removed to reduce background bacterial signal. P values were calculated using a nonparametric Kolmogorov-Smirnov test in Prism software (GraphPad).
Ireland E.V., Woznica A, & King N. (2020). Synergistic Cues from Diverse Bacteria Enhance Multicellular Development in a Choanoflagellate. Applied and Environmental Microbiology, 86(11), e02920-19.
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