Ten globus pallidus tissues (five from the sham group and five from the ICH group) were used to extract total RNA to detect microarray. The quantity and quality of total RNA were validated by NanoDrop ND-1000. Before microarray scanning, total RNA was purified by removing rRNAs (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicenter) to obtain mRNAs. Next, the samples were amplified and transcribed into fluorescent cRNA (Arraystar Flash RNA Labeling Kit, Arraystar) and purified by RNeasy Mini Kit (Qiagen) subsequently. Arraystar Mouse lncRNA Microarray (V4.0, including about 22,692 mRNAs and 37,949 lncRNAs) was designed to profile mRNA along with lncRNA systematically. Agilent DNA Microarray Scanner (part number G2505C) was used for hybridized array scanning. The acquired array images were analyzed by Agilent Feature Extraction software (version 11.0.1.1). GeneSpring GX v12.1 software package (Agilent Technologies) performed quantile normalization and subsequent data processing. Differential expression of mRNAs and lncRNAs between the two groups were identified by applying a filter of fold change (FC) ≥ 2 and value of p <0.05.
Agilent dna microarray scanner part number g2505c
Manufactured by Agilent Technologies
The Agilent DNA Microarray Scanner (part number G2505C) is a high-performance microarray scanner designed for capturing and analyzing DNA microarray data. It features advanced optics and precision scanning capabilities to accurately capture and quantify signals from DNA microarray slides.
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Lab products found in correlation
Mrna only eukaryotic mrna isolation kit, by Illumina (2 mentions)
Flash rna labeling kit, by Arraystar (2 mentions)
Genespring gx v12.1 software package, by Agilent Technologies (1 mentions)
Agilent feature extraction software, by Agilent Technologies (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
Agilent feature extraction software version 11.0.1.1, by Agilent Technologies (1 mentions)
Microarray v3, by Arraystar (1 mentions)
2 protocols using agilent dna microarray scanner part number g2505c
1
Profiling of mRNA and lncRNA in ICH
2
Transcriptomic Analysis of TNBC Cell Lines
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MDA-MB-231 and Hs578T cells were treated with 100 n M of DHT for 48 hours. The total RNA was extracted from treated or untreated TNBC cells and mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then, each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3' bias utilizing a random priming method (Arraystar Flash RNA Labeling Kit, Arraystar). The labeled cRNA was then hybridized onto the Microarray v3.0 (ArrayStar). After the washing steps, the Agilent DNA Microarray Scanner (part number G2505C) was used to scan the arrays. Acquired array images were analyzed by Agilent Feature Extraction software (version 11.0.1.1). Quantile normalization and subsequent data processing were performed using The Agilent GeneSpring GX v12.1 software package (Agilent Technologies). The threshold we used to screen dysregulated mRNAs is fold-change ≥ 2.0 and a P-value ≤ 0.05.
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