Nickel ida agarose

Mutagenic oligonucleotides were purchased from Integrated DNA Technologies. Phusion High-Fidelity DNA Polymerase, dNTPs and Dpn I were purchased from New England Biolabs. Kanamycin Monosulfate, isopropyl-β-D-thiogalactopyranoside (IPTG), tris(hydroxymethyl)aminomethane (TRIS) and Nickel-IDA agarose were purchased from Gold Biotechnology. Human neutrophil elastase was purchased from Elastin Products Company. Methoxysuccinyl-Ala-Ala-Pro-Val-P-nitroanilide (MSA₂PV-pNA), Triton X-100, dimethylsulfoxide (DMSO), succinic acid and Luria Broth powder were purchased from Sigma-Aldrich. Imidazole was purchased from Alfa Aesar. Nickel (II) Chloride-hexahydrate was purchased from Acros Organics. 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) was purchased from MP Biomedical, LLC. Plasmid DNA was purified using the Wizard Plus SV Miniprep DNA Purification System (Promega).

Kanamycin monosulfate, isopropyl-β-D-thiogalactopyranoside (IPTG), tris(hydroxymethyl)aminomethane (TRIS) and Nickel-IDA agarose were purchased from Gold Biotechnology. Methoxysuccinyl-Ala-Ala-Pro-Val-P-nitroanilide (MSA₂PV-pNA), Triton X-100, dimethylsulfoxide (DMSO), succinic acid and Luria Broth powder were purchased from Sigma-Aldrich. Nickel (II) Chloride-hexahydrate was purchased from Acros Organics. Imidazole was purchased from Alfa Aesar. 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) was purchased from MP Biomedical, LLC. Human neutrophil elastase was purchased from Elastin Products Company. Mutagenic oligonucleotides were purchased from Integrated DNA Technologies. Phusion High-Fidelity DNA Polymerase, dNTPs and Dpn I were purchased from New England Biolabs. Plasmid DNA was purified using the Wizard Plus SV Miniprep DNA Purification System (Promega).