The human thyroid epithelial cell line Nthy-ori 3-1 (NTHY), PTC cell lines TPC-1, BCPAP, and IHH4 were acquired from Procell Life Science & Technology (Wuhan, China). The human ATC cell lines KHM-5M, C643, CAL-62 and BHT101 were acquired from the National Collection of Authenticated Cell Cultures (Shanghai, China). Deutsche Sammlung von Mikroorganismen und Zellkulturen provided us with another ATC cell line called 8505C. All cells have STR Authentication and were cultured in HyClone RPMI-1640 with 10% fetal bovine serum (Cat: S-FBS-SA-015, SERANA, Germany), 100U/ml penicillin, and 0.1 mg/ml streptomycin. The cells were maintained at 37 °C with 5% CO2.
Khm 5m
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The KHM-5M is a high-performance laboratory centrifuge designed for a variety of cell culture applications. It features a maximum speed of 5,000 rpm and can accommodate a range of rotor sizes to handle different sample volumes. The centrifuge is equipped with a digital display and programmable functions for precise control over run time and speed.
Automatically generated - may contain errors
Lab products found in correlation
Cal 62, by National Collection of Authenticated Cell Cultures (4 mentions)
Bcpap, by National Collection of Authenticated Cell Cultures (2 mentions)
Nthy ori3 1, by National Collection of Authenticated Cell Cultures (2 mentions)
Khm 5m, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Nthy ori 3 1, by Procell (1 mentions)
Tpc 1, by Procell (1 mentions)
Bcpap, by Procell (1 mentions)
Mda t41, by American Type Culture Collection (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Nthy ori 3 1, by Merck Group (1 mentions)
Cal 62, by Lonza (1 mentions)
Nthy ori 3 1, by European Collection of Authenticated Cell Cultures (1 mentions)
Rpmi 1640, by Lonza (1 mentions)
Penicillin streptomycin, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Bht 101, by Thermo Fisher Scientific (1 mentions)
Nthy ori 3 1, by Thermo Fisher Scientific (1 mentions)
Bcpap, by Thermo Fisher Scientific (1 mentions)
Cal 62, by Thermo Fisher Scientific (1 mentions)
5 protocols using khm 5m
1
Culturing Thyroid Cancer Cell Lines
2
Thyroid Cancer Cell Line Cultivation
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Thyroid cancer cell lines, including BCPAP, KTC-1, KHM-5M, HTh7, ACT-1, CAL-62, C643, WRO, and TTA-1, as well as normal human primary thyroid follicular epithelial cell Nthy-ori 3-1, were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). The human thyroid cancer cell line MDA-T41 was purchased from the American Type Culture Collection (Manassas, USA). BCPAP, KHM-5M, MDA-T41, C643, and Nthy-ori 3-1 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS). KTC-1 and WRO cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium supplemented with 10% FBS, 1% nonessential amino acids, and 1% sodium pyruvate. CAL-62, HTh7, ACT-1, and TTA-1 were cultured in Dulbecco's Modified Eagle Medium (DMEM) medium supplemented with 10% FBS. All cells were incubated at 37°C in a 5% CO 2 (carbon dioxide) environment.
3
Establishing Doxorubicin-Resistant Thyroid Cancer Cell Lines
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Human normal thyroid cell line (Nthy-ori 3-1) was purchased from the European Collection of Authenticated Cell Cultures (ECACC). Human ATC cell lines, including FRO, 8505C, C643, CAL-62, and KHM-5M, were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). Cells were maintained in DMEM (Lonza, Swiss) (8505C and CAL-62) or RPMI-1640 medium (Lonza, Swiss) (Nthy-ori 3-1, FRO, C643, and KHM-5M) containing 10% fetal bovine serum (FBS) (Sigma, USA) and 1% penicillin/streptomycin (Beyotime, China) at 37°C in an incubator with 5% CO2. The doxorubicin-resistant 8505C cells (8505C/Dox) were established from the parental 8505C cells by exposing cells to gradually increasing concentrations of doxorubicin (Sigma, USA) from 0.1 μM to 10 μM over 8 months (14 (link)). 8505C/Dox cells were cultured in culture medium containing 2 μM doxorubicin to maintain drug resistance phenotype.
4
Thyroid Cancer Cell Lines and Tissues
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Human thyroid cancer cell lines CAL-62, KHM-5M, BHT-101, B-CPAP, and normal thyroid cell lines Nthy-ori-3-1 were purchased from the China Center for Type Culture Collection (CCTCC, China). The CAL-62 and BHT-101 cells were cultured in DMEM medium supplemented with 10% and 20% fetal bovine serum (Gibco, USA) at 37°C in 5% CO2, respectively. KHM-5M, B-CPAP and Nthy-ori-3-1 were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Gibco, USA) at 37°C in 5% CO2. A total 15 of DTC patients who underwent radical thyroidectomy in Ruijin Hospital of Shanghai Jiaotong University Medical College and were confirmed as DTC by postoperative histopathological examination were included in this study. All samples were obtained with the patients’ informed consent, and the samples were histologically confirmed by at least 2 pathologists independently in a double-blinded fashion.
5
Investigating Apatinib's Effects on ATC Cells
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Human ATC cell lines KHM-5M and C643 were purchased from the China Center for Type Culture Collection (CCATCC, China). The C643 and KHM-5M cells were cultured in RPMI-1640 medium supplemented with 10% foetal bovine serum (Gibco, USA) at 37 °C in 5% CO2 (Shanghai Medical Instruments, China). Apatinib was obtained from Hengrui Medicine Co. Ltd. (Jiangsu, China), dissolved in DMSO and diluted with 1640 medium to the desired concentration with a final DMSO concentration of 0.1% for in vitro studies. Prior to each treatment, cells were plated overnight and displayed a similar subconfluently density at the time of drug exposure. The SC79, CQ, and rapamycin were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA) and were dissolved in PBS and diluted with RPMI-1640 to the desired concentration. Bafilomycin A1 (Baf A1) was obtained from Selleck Chemicals (Houston, TX, USA).
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