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Mda mb 231 adr

Manufactured by Thermo Fisher Scientific
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The MDA-MB-231-ADR is a cell line derived from the MDA-MB-231 breast cancer cell line. It is an adriamycin (doxorubicin)-resistant variant of the original MDA-MB-231 cells.

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4 protocols using mda mb 231 adr

1

Culturing MDA-MB-231 Breast Cancer Cells

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MDA-MB-231 was purchased from the American Type Culture Collection (ATCC), MDA-MB-231-ADR was purchased from Shanghai Chunshi Biotechnology co. LTD. The MDA-MB-231 cells were cultured in DMEM containing 10% FBS (Thermo Fisher Scientific, Waltham, MA, USA), while the MDA-MB-231-ADR was cultured in L15 containing 10% fetal bovine serum (FBS). All the cells were cultured in a humidified 5% CO2 incubator at 37 °C.
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2

Evaluating FABP7 mRNA in Breast Cancer

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The association between FABP7 mRNA level and survival outcomes of breast cancer patients was evaluated by Kaplan-Meier plotter (http://kmplot.com), which is an online public database that includes 5,143 breast cases [15] . The hazard ratio (HR) and log rank P-value was displayed on the webpage. The database divides all patients into different molecular subtypes according to the Sorlie's subtypes and the long-rank tests was used to obtain the hazard ratio (HR) and P-value.
Cell lines and cell culture MDA-MB-231 was purchased from the American Type Culture Collection (ATCC), MDA-MB-231-ADR was purchased from Shanghai Chunshi Biotechnology co. LTD. The MDA-MB-231 cells were cultured in DMEM containing 10% FBS (Thermo Fisher Scientifc, Waltham, MA, USA), while the MDA-MB-231-ADR was cultured in L15 containing 10% fetal bovine serum (FBS). All the cells were cultured in a humidi ed 5% CO2 incubator at 37°C.
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3

Cell Culture Protocols for MDA-MB-231 and MDA-MB-231-ADR

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MDA-MB-231 was purchased from the American Type Culture Collection (ATCC), MDA-MB-231-ADR was purchased from Shanghai Chunshi Biotechnology co. LTD. The MDA-MB-231 cells were cultured in DMEM containing 10% FBS (Thermo Fisher Scientifc, Waltham, MA, USA), while the MDA-MB-231-ADR was cultured in L15 containing 10% fetal bovine serum (FBS). All the cells were cultured in a humidified 5% CO2 incubator at 37°C.
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4

Evaluating FABP7 mRNA in Breast Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
The association between FABP7 mRNA level and survival outcomes of breast cancer patients was evaluated by Kaplan-Meier plotter (http://kmplot.com), which is an online public database that includes 5,143 breast cases [15] . The hazard ratio (HR) and log rank P-value was displayed on the webpage. The database divides all patients into different molecular subtypes according to the Sorlie's subtypes and the long-rank tests was used to obtain the hazard ratio (HR) and P-value.
Cell lines and cell culture MDA-MB-231 was purchased from the American Type Culture Collection (ATCC), MDA-MB-231-ADR was purchased from Shanghai Chunshi Biotechnology co. LTD. The MDA-MB-231 cells were cultured in DMEM containing 10% FBS (Thermo Fisher Scientifc, Waltham, MA, USA), while the MDA-MB-231-ADR was cultured in L15 containing 10% fetal bovine serum (FBS). All the cells were cultured in a humidi ed 5% CO2 incubator at 37°C.
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