MSCs surface marker expression on hPDLSCs was evaluated using flow cytometry analysis (n = 4). The antibodies were FITC-conjugated anti-human CD105 antibody (Bio Legend, USA), FITC-conjugated anti-human CD73 antibody (Bio Legend, USA), FITC-conjugated anti-human CD90 antibody (Bio Legend, USA), FITC-conjugated anti-human CD44 antibody (Bio Legend, USA), and FITC-conjugated anti-human CD45 antibody (Bio Legend, USA). FITC-conjugated mouse IgG1κ Isotype was employed as the isotype control. The assay was performed by utilizing FACS caliber flow cytometer (BD Biosciences, USA).
Fitc conjugated anti human cd105 antibody
Manufactured by BioLegend
Sourced in United States
The FITC-conjugated anti-human CD105 antibody is a laboratory reagent used to detect and analyze the expression of CD105 (endoglin) on human cells. CD105 is a cell surface receptor involved in angiogenesis and cell proliferation. This antibody is conjugated with the fluorescent dye FITC, which enables the identification and quantification of CD105-positive cells using flow cytometry or other fluorescence-based techniques.
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Lab products found in correlation
Fitc conjugated anti human cd45 antibody, by BioLegend (2 mentions)
Fitc conjugated anti human cd44 antibody, by BioLegend (2 mentions)
Facs caliber flow cytometer, by BD (1 mentions)
Fitc conjugated anti human cd73 antibody, by BioLegend (1 mentions)
Facscallibur flow cytometer, by BD (1 mentions)
Fitc conjugated anti human cd90 thy1 antibody, by BioLegend (1 mentions)
Cellquest software, by BD (1 mentions)
2 protocols using fitc conjugated anti human cd105 antibody
1
Characterization of hPDLSCs Markers
2
Characterization of Human Dental Pulp Stem Cells
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hDPSCs were characterized according cell morphology, stemness and proliferative mRNA marker expression, and surface marker analysis. Cell morphology was captured by phase-contrast microscope. RT-qPCR was used to analysed mRNA marker expression regarding stemness property (REX1, NANOG, and OCT4) and proliferative marker (Ki67). Cells were then characterized by flow cytometry for MSC surface markers. Briefly, the cells were stained with FITC-conjugated anti-human CD105+ antibody (Bio Legend, California, USA), FITC-conjugated anti-human CD73+ (Ecto-5′-nucleotidase) antibody (Bio Legend, California, USA), FITC-conjugated anti-human CD90+ (Thy1) antibody (Bio Legend, California, USA), FITC-conjugated anti-human CD44+ antibody (Bio Legend, California, USA), and FITC-conjugated anti-human CD45− antibody (Bio Legend, California, USA). FITC-conjugated Mouse IgG1κ isotype control (FC) antibody (Bio Legend, California, USA) was used as an isotype control for this assay. The assay was performed by using FACScallibur flow cytometer with CellQuest software (BD Bioscience, New Jersey, USA).
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