Phoenix ganzfeld erg

Scotopic ERG and photopic ERG were measured at 5 w and 12 w in mice. ERG was recorded according to the manufacturer’s instructions for the Phoenix Ganzfeld ERG (Phoenix Research Laboratories, Pleasanton, CA). All animals underwent dark adaptation overnight before ERG recording. After anesthesia, the mice were placed on the heating pad, and ERG was recorded after pupil dilation. The reference electrode was placed subcutaneously in the forehead between the ears, and the ground electrode was placed subcutaneously in the tail. Corneal electrodes were placed on the cornea after application of 2.5% hypromellose. Scotopic ERG was recorded at the following increasing stimulus intensities: −1.1, 0.1, 0.7, 1.0, 1.3, and 2.2 log cd s/m². Photopic ERGs were stimulated with green light (wavelength, 504 nm) and UV light (wavelength, 365 nm), and photopic recordings were performed after a 5-min light adaptation interval at a background light intensity of 1.3 log cd s/m², which also served as background light for the duration of the optical recording. Photopic ERG was recorded at the following increasing stimulus intensities: 0.3, 0.9, 1.5, 2.1, 3, and 3.9 log cd s/m².

Scotopic ERG was measured 4 weeks and 12 weeks post subretinal injection. ERG was recorded under the manufacturer's instructions of the Phoenix Ganzfeld ERG (Phoenix Research Labs). Briefly, mice were dark adapted for 16 h and then all the preparations were operated under dim red light. After anesthesia, mice were placed on a heating pad to maintain body temperature. The pupils were dilated. The reference electrode was placed subcutaneously in the forehead between the ears, and the ground electrode was placed subcutaneously in the tail. A corneal electrode was placed on the cornea after applying 2.5% Hypromellose. ERG was recorded with stimulus intensity at 1.2 log cd s/m².