For the extraction of the community DNA 2.0 L of contaminated groundwater was filtered through 0.22 μm pore size cellulose nitrate filter (Millipore, Billerica, USA). Before the transferring procedure 2 mL enrichment samples were collected using a sterile syringe purged with nitrogen gas. Enrichment samples were centrifuged at 12,000×g for 5 min. DNA was extracted from membrane filters and from the pellets derived from groundwater and enrichments, respectively, using Ultraclean™ Soil DNA Isolation Kit (MoBio Laboratories Inc. Carlsbad, USA) according to manufacturer’s instructions, with a modification at the physical cell disruption step. The samples were shaken in “Bead Solution tubes” (MoBio Laboratories) containing microbeads at 25 Hz for 2 min using mixer mill MM301 (Retsch, Haan, Germany).
Bead solution tubes
Manufactured by Qiagen
Sourced in Germany
Bead Solution tubes are laboratory equipment designed to hold and contain a solution of beads. The core function of these tubes is to provide a secure and controlled environment for storing and working with bead-based samples or reagents during laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using bead solution tubes
1
Groundwater DNA Extraction Protocol
2
Genomic DNA Extraction from Nitrifying Enrichments
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After incubation, the total volume of nitrifying enrichments resulting positive reaction for ammonia or nitrite oxidation and 2 L water samples were filtered on 0.22 μm pore size cellulose nitrate filters (Millipore, Billerica, USA). From samples concentrated on membranes, genomic DNA was extracted using Ultraclean TM Soil DNA Isolation Kit (Mo Bio Laboratories, Inc., USA) according to the manufacturer's protocol, supplemented by physical cell disruption. The membranes were shaken in "Bead Solution tubes" (Mo Bio Laboratories) containing microbeads at 25 Hz for 2 min with cell mill MM301 (Retsch, Haan, Germany) to enhance cell disruption.
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