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The VS-DBS is a laboratory instrument designed for deep brain stimulation (DBS) procedures. It provides a reliable and precise method for the implantation of DBS electrodes into the brain. The core function of the VS-DBS is to assist healthcare professionals in the accurate placement of DBS electrodes during surgical interventions.

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Lab products found in correlation

Volumescope serial block face em, by Thermo Fisher Scientific (2 mentions) W1503, by Merck Group (1 mentions) Maps software, by Thermo Fisher Scientific (1 mentions)

2 protocols using vs dbs

1

Embryo Fixation and Serial Block-Face SEM

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Embryos were fixed in 2.5% glutaraldehyde in embryo grade water (Sigma, W1503) for 1–2 h at room temperature. Fixed embryos were rinsed with 1x PBS and stored at 4 °C before processing. Processing was performed exactly as described previously76 (link). Serial blockface sectioning and scanning electron microscopy were performed using a VolumeScope serial block-face EM (Thermo Fisher, Waltham, MA) equipped with a low-vacuum backscatter detector (VS-DBS; Thermo Fisher).
Hawdon A., Geoghegan N.D., Mohenska M., Elsenhans A., Ferguson C., Polo J.M., Parton R.G, & Zenker J. (2023). Apicobasal RNA asymmetries regulate cell fate in the early mouse embryo. Nature Communications, 14, 2909.
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2

Serial Block-face Electron Microscopy

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Images were additionally collected using a VolumeScope serial block-face EM (SBEM; Thermo Fisher, Waltham, MA) equipped with a low-vac backscatter detector (VS-DBS; Thermo Fisher). Plastic embedded samples were scanned in low vacuum (10 Pa) with a landing beam energy of 2.0 kV and a current of 0.1 nA. Images were acquired using MAPS software (Thermo Fisher, Waltham, MA) at a pixel scale of 5.9–7 nm and pixel dwell of 3 µs.
Rae J., Ferguson C., Ariotti N., Webb R.I., Cheng H.H., Mead J.L., Riches J.D., Hunter D.J., Martel N., Baltos J., Christopoulos A., Bryce N.S., Cagigas M.L., Fonseka S., Sayre M.E., Hardeman E.C., Gunning P.W., Gambin Y., Hall T.E, & Parton R.G. (2021). A robust method for particulate detection of a genetic tag for 3D electron microscopy. eLife, 10, e64630.
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