Sodium benzoate

Manufactured by Thermo Fisher Scientific

Sourced in United States

Sodium benzoate is a chemical compound commonly used as a food preservative. It is a white crystalline powder that is soluble in water. Sodium benzoate is used to inhibit the growth of mold, yeast, and bacteria in a variety of food and beverage products.

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Lab products found in correlation

6 protocols using sodium benzoate

Polyphenol Extraction from Berberis and Carrot

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Berberis boliviana freeze-dried berries were generously donated by Carla del Carpio from Universidad Nacional de San Antonio Abad del Cusco (UNSAAC, Cusco, Peru). A commercial black carrot color powder (Daucus carota) was provided by D.D. Williamson (Louisville, KY, USA). CA, FA, SA, and PA were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetone, chloroform, acetonitrile, UHPLC-grade water, pCA, sodium benzoate, and sodium hydroxide were obtained from Fisher Scientific (Pittsburgh, PA, USA). Potassium sorbate was obtained from Spectrum (New Brunswick, NJ, USA). All other reagents and solvents were of at least analytical or HPLC grade unless otherwise indicated.

Miyagusuku-Cruzado G., Voss D.M, & Giusti M.M. (2021). Influence of the Anthocyanin and Cofactor Structure on the Formation Efficiency of Naturally Derived Pyranoanthocyanins. International Journal of Molecular Sciences, 22(13), 6708.

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Formulation and Stability of Rebaudioside A Beverage

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A mixture of 300 g deuterium oxide (99% D) (Sigma Aldrich, Saint Louis, MO, USA), 540 mg rebaudioside A, 135 mg citric acid, 27 mg malic acid, 54 mg potassium sorbate, and 27 mg sodium benzoate (Fisher Scientific, Waltham, MA, USA) was prepared in a 250 mL bottle (pH = 3.5). Dry nitrogen was purged to remove oxygen, and the sample was stored at 35 °C for 6 weeks. Samples were prepared aseptically in a climate-controlled hood and monitored for microbial growth during storage.

Gelinas B.S., Tello E, & Peterson D.G. (2022). Mechanisms of Rebaudioside A Degradation and Ingredient-Sweetener Interactions in Beverages during Storage. Molecules, 27(4), 1385.

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Analytical Characterization of PAHs

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The naphthalene, fluoranthene, pyrene and chrysene of analytical grades were purchased from Sigma Aldrich Corp. (St. Louis, MO, USA). Sodium benzoate (99+% purity), 2,2,4,4,6,8,8-heptamethylnonane (HMN), and all other organic solvents were obtained from Fisher Scientific Co. (Springfield, NJ, USA). Hexane, a high purity solvent for GC-chromatograph was obtained from EMD Chemicals Inc. Merck. The PAH analytical standards were procured from Accustandard Inc. (New Haven, CT 06513). All other chemicals and reagents used were of reagent grade or better.

Nwinyi O.C., Ajayi O.O, & Amund O.O. (2016). Degradation of polynuclear aromatic hydrocarbons by two strains of Pseudomonas. Brazilian Journal of Microbiology, 47(3), 551-562.

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Protein extraction and characterization

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GK from Sterculia urens was obtained from Nutriroma Co. (Hyderabad, Andhra Pradesh, India). SPI was purchased from Protein Technologies International (St. Louis, MO, USA). Canola oil was bought from a local supermarket (Selangor, Malaysia). Citric acid was supplied by Riedel-de Haën (Victoria, Australia). In this study, NaCl, acrylamide gels, methanol, acetic acid and Coomassie Brilliant Blue (R-250) were purchased from Merck KGaA (Darmstadt, Germany). NaOH, HCl, Tris-HCl buffer (pH 6.8), mercaptoethanol, bromophenol blue and sodium benzoate were supplied by Fisher Scientific (Pittsburgh, PA, USA).

Shekarforoush E., Mirhosseini H., Sarker M.Z.I., Kostadinović S., Ghazali H.M., Muhamad K, & Samaram S. (2016). Soy Protein–Gum Karaya Conjugate: Emulsifying Activity and Rheological Behavior in Aqueous System and Oil in Water Emulsion. Journal of the American Oil Chemists' Society, 93(1).

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Quantification of Bioactive Compounds

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Isosweet 100 high-fructose corn syrup was obtained from Tate & Lyle (Decatur, IL, USA), potassium sorbate from Spectrum (Gardena, CA, USA), sodium benzoate from Alfa Aesar (Heysham, Lancaster, UK), and sodium bicarbonate from Sigma Chemical Co. (St. Louis, MO, USA). Citric acid, mass spectrometry (MS) grade water, acetonitrile, methanol, formic acid, hydrochloric acid, and high pressure liquid chromatography (HPLC) grade acetone were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Chloroform and ethyl acetate were obtained from Mallinckrodt Chemicals (Phillipsburg, NJ, USA). CGA and FA were purchased from MP Biochemicals, LLC (Solon, OH, USA). All reagents were of analytical grade unless otherwise indicated.

Pangestu N.P., Miyagusuku-Cruzado G, & Giusti M.M. (2020). Copigmentation with Chlorogenic and Ferulic Acid Affected Color and Anthocyanin Stability in Model Beverages Colored with Sambucus peruviana, Sambucus nigra, and Daucus carota during Storage. Foods, 9(10), 1476.

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Analytical Reagents and Materials Sourcing

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All the solvents were of analytical grade purity: acetonitrile, ethyl acetate, methanol, sodium carbonate, sodium chloride, sodium hydroxide, potassium hydroxide, potassium persulfate, Fe(NH 4 ) 2 (SO 4 ) 2 , EDTA, acetic acid, formic acid, p-dimethylaminobenzaldehyde (DMAB), Carrez I, Carrez II, H 3 PO 4 , HCl, sodium molybdate, H 2 SO 4 , H 2 O 2 were purchased from VWR International Eurolab, part of Avantor (Llinars del Vallès, Cataluña, Spain). Gallic acid (GA), NaNO 2, Na 2 SO 4 and catechin (Ct) were purchased from Panreac (Barcelona, Cataluña, Spain). AlCl 3 and fluorescein sodium were purchased from Fluka Chemie GmbH, part of Sigma-Aldrich (Buchs, San Galo, Switzerland). Na 2 HPO 4 , NaH 2 PO 4 were purchased from Scharlab (Sentmenat, Cataluña, Spain).
Potassium tetraborate, nitro-blue tetrazolium (NBT), sodium benzoate and uric acid (UA) were purchased from Alfa Aesar, part of Thermo Fisher (Kandel, Rheinland-Pfalz, Germany). Nutrient hyaluronidase from bovine testes type IV-S (1400 U/ml, H3884) were purchased from Sigma-Aldrich, part of Merck (Steinheim, Nordrhein-Westfalen, Germany). Water was deionized using a Milli-Q water purification system (Millipore, part of Merck, Bedford, MA, USA).

Osés S.M., Nieto‐Ortega S., Rodrigo S., Pérez S., Rojo-Tolosa S., Sancho M.T., & Fernández‐Muiño M.A. (2020). Authentication of strawberry tree (Arbutus unedo L.) honeys from southern Europe based on compositional parameters and biological activities. Food Bioscience, 38, 100768-100768.

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