Myogenin f5d

Manufactured by Agilent Technologies

Sourced in United States

Myogenin (F5D) is a mouse monoclonal antibody that targets the myogenin protein. Myogenin is a transcription factor that plays a crucial role in the regulation of skeletal muscle development and differentiation. This antibody can be used in various research applications to study the expression and function of myogenin in different cell and tissue types.

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Lab products found in correlation

Ae1 ae3, by Agilent Technologies (1 mentions) Ventanabenchmark xt automatic, by Roche (1 mentions) Bcor c 10, by Santa Cruz Biotechnology (1 mentions) Cd34 qbend 10, by Agilent Technologies (1 mentions) Desmin d33, by Agilent Technologies (1 mentions) Ema e29, by Agilent Technologies (1 mentions) Ini 1, by BD (1 mentions) Ultraview universal dab detection kit, by Roche (1 mentions)

2 protocols using myogenin f5d

Immunohistochemical Analysis of Soft Tissue Tumors

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Sections were cut at 4 μm thick from formalin-fixed-paraffin-embedded (FFPE) tissue and immunostained using a VentanaBenchmark XT automatic stainer (Ventana, Tuscon, AZ, USA). Signals were revealed using the ultraView Universal Dab Detection kit (Ventana). The following antibodies were used: CD99 (12E7, Dako), EMA (E29, 1:50, Dako), desmin (D33, 1:80, Dako), cytokeratin AE1/AE3 (AE1/AE3, 1:50, Dako), caldesmon (h-CD, 1:100, Dako), myogenin (F5D, 1:100, Dako), S100 protein (Z0311, 1:800, Dako), CD34 (QBend-10, 1:25, Dako), INI1 (25, 1:50, BD Transduction Laboratories), BCOR (C-10, 1:50, Santa Cruz), ETV4 (16, 1:50, Santa Cruz). Immunohistochemistry for NTRK1 was performed using a 32 min incubation with the NTRK1 antibody (ab76291, clot EP1058Y, Abcam, dilution 1:200) on a Ventana ULTRA machine with Cell Conditioning Solution 1 pre-treatment for 64 min.

Alholle A., Karanian M., Brini A.T., Morris M.R., Kannappan V., Niada S., Niblett A., Ranchère-Vince D., Pissaloux D., Delfour C., Gonzalez A.M., Antonescu C.R., Sumathi V., Tirode F, & Latif F. (2018). Genetic analyses of undifferentiated small round cell sarcoma identifies a novel sarcoma subtype with a recurrent CRTC1-SS18 gene fusion. The Journal of pathology, 245(2), 186-196.

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Immunohistochemical Profiling of Muscle Markers

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Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue sections from each case using antibody-specific epitope retrieval techniques with Dako Envision (Dako, Carpinteria, CA, USA) automated system for detection of the following primary antigens: Desmin (DE-R-11, 1:100, Leica, Newcastle Upon Tyne, UK), MyoD1 (EP212, 1:25-1:100, Cell Marque, Rocklin, CA, USA), Myogenin (F5D, 1:25-1:50, Dako), and CD163 (10D6, 1:200, Leica, Newcastle Upon Tyne, UK).

Martinez A.P., Fritchie K.J., Weiss S.W., Agaimy A., Haller F., Huang H.Y., Lee S., Bahrami A, & Folpe A.L. (2019). Histiocyte-rich rhabdomyoblastic tumor: rhabdomyosarcoma, rhabdomyoma, or rhabdomyoblastic tumor of uncertain malignant potential? A histologically distinctive rhabdomyoblastic tumor in search of a place in the classification of skeletal muscle neoplasms. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 32(3).

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