Wt sense target labeling and control reagents kit

RNA labeling and microarray hybridization methods have been previously reported [34 (link)]. Briefly, 300 ng of total RNA were amplified and labeled using the WT Sense Target labeling and control reagents kit (Affymetrix, Santa Clara, CA, USA), and then hybridized to Human Gene 1.0 ST Array (Affymetrix). Washing and scanning were carried out using the Affymetrix GeneChip System (Gene-Chip Hybridization Oven 640, GeneChip Fluidics Station 450 and GeneChip Scanner 7G).
Complete microarray data are available from the Gene Expression Omnibus (www.ncbi.nlm.nih.gov/geo/, accession number GSE77540).

WAR microarray data were obtained during the study performed by López-López et al. (18 (link)). Briefly, RNA was extracted from the IC of seven WAR and three Wistar rats previously subjected to acoustic stimulation. After RNA purification and quantification, the RNA was amplified subsequently, using the WT Sense Target labeling and control reagents kit (Affymetrix Inc.), and then hybridized to rat microarrays (Gene 1.0 ST Array). Following scanning and image analysis, the microarray data were imported and the significance analysis of microarrays (SAM algorithm) was used to identify significant differential expression between the experimental groups. We selected the genes that vary in a range fold change greater than or equal to two (|FC| ≥ 2) among other genes and p < 0.05 (p ≤ 0.05). The functional annotation of the selected genes was performed using the Gene Ontology (GO) Consortium database. The microarray WAR data were deposited in the NCBI's Gene Expression Omnibus at GEO Series accession numbers GSE74150 (18 (link)).