Anti osteocalcin

Manufactured by Merck Group

Sourced in United Kingdom

Anti-osteocalcin is a laboratory reagent used in the detection and quantification of osteocalcin, a protein found in bone. It is commonly used in research and clinical settings to assess bone metabolism and turnover.

Automatically generated - may contain errors

Lab products found in correlation

Anti vegf, by Abcam (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Roche (1 mentions) Osteogenesis assay kit, by Merck Group (1 mentions) Vectastain abc system, by Vector Laboratories (1 mentions) Image pro plus software, by Zeiss (1 mentions) Standard light microscopy, by Zeiss (1 mentions) Mab353, by Merck Group (1 mentions) Anti aggrecan neo epitope, by Novus Biologicals (1 mentions) Anti col2a1, by Santa Cruz Biotechnology (1 mentions) Anti mmp13, by Abcam (1 mentions) Pannormic midi, by 3DHISTECH (1 mentions) Anti osterix, by Abcam (1 mentions) Bx61w1 fv1000, by Olympus (1 mentions) Anti p smad2, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Osteocalcin (8 citations)

3 protocols using anti osteocalcin

Osteoblast Differentiation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Alkaline phosphatase (ALP) specific activity was used as an early marker of the osteoblastic phenotype. Therefore, cells were washed in PBS and fixed in 4% paraformaldehyde for 5 min. After washing, cells were incubated with 0.1% naphthol AS-MX phosphate and 0.1% fast red violet LB salt in a 2-amino-2-methyl-1,3-propanediol buffer (56 mM) for 10 min. Alizarin red S staining to visualize calcification was performed according to manufacturer’s protocol (Osteogenesis assay kit, #ECM815, Millipore). Matrix mineralization was determined by immunofluorescence labeling with anti-osteocalcin (#AB10911, Millipore). As secondary antibody Alexa 488-labeled secondary goat anti-rabbit antibody (Molecular Probes, USA, 1:100) was used. After staining cells were counterstained with DAPI (Roche Diagnostics GmbH, Germany) for 15 min.

Engel N., Adamus A., Schauer N., Kühn J., Nebe B., Seitz G, & Kraft K. (2017). Synergistic Action of Genistein and Calcitriol in Immature Osteosarcoma MG-63 Cells by SGPL1 Up-Regulation. PLoS ONE, 12(1), e0169742.

+ Open protocol

+ Expand

Histological Analysis of Mouse Bone Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissues were fixed in 4% paraformaldehyde and decalcified in 10% EDTA. Paraffin sections were stained with Masson’s trichrome stain for morphological analysis. For IHC, antigens of de-paraffinized sections were retrieved by 0.05% trypsin or hyaluronidase (10 mg/ml) and treated with 3% H₂O₂. After blocking with 5% normal goat serum, tissues were incubated with primary antibodies in 4°C, overnight. The following rabbit polyclonal antibodies against mouse were used, anti-VEGF (Abcam, Cambridge, UK), anti-PECAM (Abcam), and anti-Osteocalcin (Millipore, Billerica, MA, USA). Sections were then incubated with anti-rabbit secondary antibody (Vectastain ABC system, Vector Laboratories, Servion, Switzerland) and developed with 0.1% 3, 39-diaminobenzidine. Images were captured using standard light microscopy (Zeiss, Oberkochen, Germany) and quantified using Image-Pro Plus software (Rockville, MD, USA). Data from three independent mice staining were used for statistical analysis.

Song I.W., Li W.R., Chen L.Y., Shen L.F., Liu K.M., Yen J.J., Chen Y.J., Chen Y.J., Kraus V.B., Wu J.Y., Lee M.T, & Chen Y.T. (2014). Palmitoyl Acyltransferase, Zdhhc13, Facilitates Bone Mass Acquisition by Regulating Postnatal Epiphyseal Development and Endochondral Ossification: A Mouse Model. PLoS ONE, 9(3), e92194.

+ Open protocol

+ Expand

Histological and Immunohistochemical Assessment of Joint Development

Check if the same lab product or an alternative is used in the 5 most similar protocols

After sampling for genotyping, the limbs were isolated and immediately fixed in 4% buffered paraformaldehyde for 24 h, decalcified in 14% EDTA (pH 7.4) for 3 days then embedded in paraffin; 6 mm sagittal joint sections were processed for H&E staining.
Immunostaining was performed using a standard protocol as previously described 25 . Primary antibodies were: anti-MINK1 (NO.NBP1-22990, Novus Biologicals), anti-COL2A1 (NO.sc-52658, Santa Cruz Biotechnology Inc.), anti-Aggrecan Neoepitope (NO.NBP100-74350, Novus Biologicals), anti-pSMAD2 (NO.3108, Cell Signaling Technology), anti-osterix (NO.ab22552, Abcam), antiosteocalcin (NO.AB10911, Millipore), anti-VEGF (NO.ab46154, Abcam), anti-MMP13 (NO. ab39012, Abcam), anti-ADAMTS-5 (NO. ab41037, Abcam), anti-COLX (NO.ab58632, Abcam) and anti-nestin (NO.MAB353, Millipore). Stained specimens were photographed digitally under confocal microscopy (BX61W1-FV1000; Olympus) or digital slide scanners (Pannormic MIDI; 3DHISTECH). Mouse IgG or rabbit IgG were used for negative controls when performing immunohistochemistry or immunofluorescence staining (Supplementary Fig. S7).

Yu D., Hu J., Sheng Z., Fu G., Wang Y., Chen Y., Pan Z., Zhang X., Wu Y., Sun H., Dai J., Lu L, & Ouyang H. (2020). Dual roles of misshapen/NIK-related kinase (MINK1) in osteoarthritis subtypes through the activation of TGFβ signaling. Osteoarthritis and cartilage, 28(1).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!