The hematological lineage of mouse BM cells and immortalized cell lines was determined using flow cytometry (FACS-Calibur or CantoII, Becton-Dickinson, Mountain View, CA), which employed phycoerythrin (PE)-Mac1, PE-CD115, PE-CD19, PE-B220, PE-Cy7-c-kit, allophycocyanin (APC)-Sca1, APC-CD3, APC-CD14 (eBioscience, San Diego, CA), and APC-Ly6G (Miltenyi Biotec, Auburn, CA, USA). The liver, spleen, and lymph node collected from the moribund mice were fixed in buffered formalin, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (H&E) using the standard technique. Stamp specimens of the tissues, PB smears, and BM cytospin preparations were stained with Liu reagents (Handsel Technologies, Taipei, Taiwan).
Apc sca1
Manufactured by Thermo Fisher Scientific
Sourced in United States
The APC)-Sca1 is a flow cytometry reagent that detects the expression of the Sca1 cell surface marker. It is a useful tool for the analysis and identification of stem and progenitor cells.
Automatically generated - may contain errors
Lab products found in correlation
B220 pe, by Thermo Fisher Scientific (2 mentions)
Facscalibur, by BD (1 mentions)
Canto 2, by BD (1 mentions)
Cd3 apc, by Thermo Fisher Scientific (1 mentions)
Cd14 apc, by Thermo Fisher Scientific (1 mentions)
Cd45 pe cy7, by Thermo Fisher Scientific (1 mentions)
Cd19 pecy7, by Thermo Fisher Scientific (1 mentions)
Cd19 apc, by Thermo Fisher Scientific (1 mentions)
Cd3 fitc, by Thermo Fisher Scientific (1 mentions)
Foxp3 pe, by Thermo Fisher Scientific (1 mentions)
Cd4 apc, by BioLegend (1 mentions)
C kit apc, by Thermo Fisher Scientific (1 mentions)
Cd45 apc cy7, by Thermo Fisher Scientific (1 mentions)
Cd8 percp, by BioLegend (1 mentions)
Cd62l fitc, by BioLegend (1 mentions)
Igm fitc, by Thermo Fisher Scientific (1 mentions)
Cd8 pe cy7, by BioLegend (1 mentions)
Cd4 apc cy7, by BioLegend (1 mentions)
2 protocols using apc sca1
1
Phenotyping Mouse Bone Marrow Cells
2
Multiparameter Flow Cytometry Immunophenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The surface markers included FITC‐CD3 (MA1‐7640, eBioscience, San Diego, CA, USA), APC‐Cy7‐CD4 (100413, Biolegend, San Diego, CA, USA), APC‐CD4 (100411, Biolegend), PerCP‐CD8 (100,731, Biolegend), PE‐Cy7‐CD8 (100721, Biolegend), PE‐Foxp3 (12‐5773‐82, eBioscience), APC‐Helios (17‐9883‐42, eBioscience), PerCP‐CD44 (103035, Biolegend), FITC‐CD62L (104405, Biolegend), FITC‐lin (22‐7770‐72, eBioscience), PerCP‐CD34 (50‐0341‐82, eBioscience), APC‐Sca‐1 (17‐5981‐82, eBioscience), PE‐Sca‐1 (12‐5981‐82, eBioscience), APC‐c‐kit (17‐1171‐82, eBioscience), PE‐c‐kit (12‐1171‐82, eBioscience), PE‐B220 (12‐0452‐82, eBioscience), FITC‐IgM (11‐5790‐81, eBioscience), PE‐Cy7‐CD19 (12‐0193‐82, eBioscience), APC‐CD19 (17‐0193‐82, eBioscience), PE‐Cy7‐CD16/32 (25‐0161‐82, eBioscience), PE‐Cy7‐CD45 (25‐0451‐82, eBioscience), PerCP‐5.5‐CD45.2 (45‐0454‐82, eBioscience), and APC‐Cy7‐CD45.1 (17‐0453‐82, eBioscience) obtained from Invitrogen. Intracellular staining with PE‐Foxp3 (72‐5775‐40, eBioscience) was performed with Foxp3 staining kits (Invitrogen, Carlsbad, CA, USA).
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