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Deionized water

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Deionized water is a type of purified water that has had its mineral ions removed through a process of ion exchange. This process removes dissolved salts and other impurities, resulting in water with a very low ionic content. Deionized water is commonly used in various laboratory applications where high-purity water is required.

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7 protocols using deionized water

1

Zinc Release Kinetics of CHX-ZnCl2 Particles

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Elemental calibration standards were prepared from pure element standards (1000 ppm standards in deionized water, VWR), which were diluted in deionized water to produce 0, 1, 5, 10, 50, and 100 ppm concentrations of each ion. The zinc content of the calibration standards was measured using inductively coupled plasma-optical emission spectroscopy (ICP-OES; Varian Vista-PRO, Yarnton, UK).
ICP-OSE was employed for measuring the zinc release curve of CHX-ZnCl2 particles in AS of pH = 4 and pH = 7, according to the time points in Table S1 (up to 7 h). At each time point, samples were centrifuged, and the supernatants were collected and replaced with fresh AS. All the experiments were repeated in triplicate.
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2

Chitosan-DNA Nanocomplex Fabrication

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Medical grade chitosan 95/1000 was purchased from Heppe Medical, sodium tripolyphosphate (TPP) was obtained from Across Organics, deoxyribonucleic acid sodium salt (DNA) was acquired from MPBIO, 35% hydrochloric acid (HCl), while sodium hydroxide palettes (NaOH) and glacial acetic acid (AA) were all acquired from VWR. The following solutions were freshly prepared by using deionized water from VWR: 2M HCl, 10M NaOH, 1% and 2% (v/v) acetic acid.
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3

Formulation and Characterization of Multifunctional Nanoparticles

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Poloxamer 407 (Sigma Aldrich), poloxamer 188 (Applichem), rhodamine B (RhoB; Sigma Aldrich), Rp-8-Br-PET-cGMPS, also known as CN03 (provided by Research Institute of Sweden), Glycerol tripalmitate (GTP; Alfa Aesar), soy-bean lecithin (LCT; VWR), stearic acid (SA; BASF), 50/50 DL-lactide/glycolide copolymer (PLGA; Corbion), poly-εcaprolactone 14kDa (PCL; Sigma Aldrich), 8-aminonaphthalene-1,3,6-trisulfonic acid disodium salt (ANTS; Biotium), p-Xylene-Bis-Pyridinium Bromide (DPX; Biotium), dichloromethane (VWR), deionized water (VWR), ARPE-19 cell (ATCC), 661W cell (generously provided by Dr. Muayyad Al-Ubaidi, University of Oklahoma), Dulbecco's modified Eagle's medium and Ham's F12 nutrient mixture (DMEM/F12; Gibco), low glucose (1mg/ml) Dulbecco's modified Eagle's medium (DMEM; Gibco), fetal bovine serum (FBS; Gibco), glutamine (Sigma Aldrich), penicillin-streptomycin (Sigma Aldrich), Accutase ® solution (Sigma-Aldrich), paraformaldehyde (Sigma Aldrich), anti-Zonula occludens-1 (ZO-1) antibody (Invitrogen), goat anti-rabbit secondary antibody (Life technologies), 4',6diamidino-2-phenylindole, dihydrochloride (DAPI; Sigma Aldrich), colorimetric methylthiazolyl diphenyl-tetrazolium bromide (Sigma Aldrich). All purchased materials were used as received.
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4

Zeta-potential Measurement of LUVs

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The electrokinetic potential (ζ-potential) of LUVs was measured by a Zetasizer Nano Zen 2600 (Malvern, Worcestershire, UK) following a procedure previously described. 30 LUV dispersions were diluted 1:20 (v/v) with filtrated deionized water (0.2 µm pore size, VWR International, Radnor, Pennsylvania, USA) prior measurements and analysis were conducted at room temperature (23-25 °C).
Measurements were performed in four replicates (n=4), where each sample were measured thrice.
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5

Multilayer Polymer Thin Film Fabrication

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Poly (sodium 49styrene sulfonate) solution (PSS, M w 70,000 30 wt.% in H 2 O), Mg(NO 3 ) 2 .6H 2 O, Al(NO 3 ) 3 .9H2O, NaOH and Na 2 CO 3 were purchased from Sigma9Aldrich. Microscope quartz and glass slides used for LbL deposition were obtained from UQG Optics Ltd. and Fisher Scientific, respectively. Deionized water (15 M\.cm 91 ), sulphuric acid (95 %) and hydrogen peroxide (50 wt.% in H 2 O) were supplied from VWR.
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6

Ziziphus jujuba var. spinosa Compounds

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ZSS was gathered from Jixian County (Tianjin, China), and authorized to be ripe seeds of Ziziphus jujuba var. spinosa (Bunge) Hu ex H. F. Chow by Dr. Junbo Xie. Spinosin (Batch No. 120519, 99.2%), JuA (Batch No. 110312, 99.0%) and JuB (Batch No. 110927, 99.1%) were purchased from Chengdu Scholar Bio-tech CO., Ltd. (Chengdu, China). Piacetam (Batch No. 10071027) was from Jinshi Pharmaceutical CO., Ltd. (Tianjin, China). HPLC grade acetonitrile and deionized water were all purchased from J.T.Baker (USA). 96% formic acid was from Tedia CO., Ltd. (USA). All other chemicals were of analytical grade.
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7

Synthesis and Cytotoxicity of Polymer Nanocomposites

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The synthesis of poly(styrene-co-acrylonitrile) P(S:AN) and poly(styrene-co-acrylonitrile-acrylic acid) P(S:AN-AA) was reported in a previous work [32 (link)]. Multiwalled carbon nanotubes (MWCNT) Baytubes C150 P were obtained from Bayer Materials Science, Leverkusen, Germany. Ethanol (Alquimia Mexicana, Mexico City, Mexico), deionized water (Meyer, Mexico City, Mexico), buffer solution (Phosphate), pH 7 (J. T. Baker, Mexico City, Mexico), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Chemical, Burlington, MA, USA) were used as reactive. A549 human epithelial cell line, obtained from American Type Culture Collection (ATCC® CCL-185, Manassas, VA, USA) and maintained in F12 culture medium supplemented with fetal bovine serum at 10% (Hyclone Laboratories Inc. Logan, UT, USA), was used for cytotoxicity experiments. All materials were used without further purification.
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