Peripheral blood mononuclear cells (PBMCs) were obtained by Ficoll-Paque (GE Healthcare, USA) gradient centrifugation. Cell surface and intracellular staining was performed using the following monoclonal antibodies (mAbs): anti-TCR-Vα7.2 (Biolegend, USA), anti-CD3, anti-CD161, anti-CD69, anti-PD-1, anti-TNFα, anti-IFN-γ, anti-IL-4, anti-IL-10, anti-CCR5, anti-CXCR6 (BD Biosciences, USA). For intracellular staining, the Cytofix/CytopermTM Fixation/Permeabilization kit was utilized according to the manufacturer's specification (BD Biosciences, USA). Data were acquired by flow cytometry (BD LSRFortessa, USA), and analyzed with FlowJo software Version7.6 (Tree Star, Ashland, OR, USA).
Anti cd161
Manufactured by BD
Sourced in United States
Anti-CD161 is a monoclonal antibody that binds to the CD161 cell surface receptor. CD161 is expressed on a subset of T cells and natural killer cells. The primary function of Anti-CD161 is to detect and quantify cells expressing the CD161 antigen.
Automatically generated - may contain errors
Lab products found in correlation
Lsrfortessa, by BD (1 mentions)
Cytofix cytoperm fixation permeabilization kit, by BD (1 mentions)
Ficoll paque, by GE Healthcare (1 mentions)
Anti ccr5, by BD (1 mentions)
Anti cd69, by BD (1 mentions)
Anti cd3, by BioLegend (1 mentions)
Anti ifn γ, by BD (1 mentions)
Anti il 10, by BD (1 mentions)
Anti tcr vα7, by BioLegend (1 mentions)
Anti pd 1, by BD (1 mentions)
Anti il 4, by BD (1 mentions)
Anti tnf α, by BD (1 mentions)
Facscalibur, by BD (1 mentions)
Cellquest pro software, by BD (1 mentions)
Anti cd155, by BioLegend (1 mentions)
Anti cd107a, by BD (1 mentions)
Anti dnam 1, by BD (1 mentions)
Anti cd158b, by BD (1 mentions)
Anti cd112, by BD (1 mentions)
Pe conjugated anti nkp30, by BD (1 mentions)
2 protocols using anti cd161
1
Profiling MAIT Cell Responses in PBMCs
2
NK-cell Phenotyping and Ligand Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For NK-cell phenotyping, 2.0 X10 5 PBMC were incubated with the appropriated Abs: FITC-conjugated anti-CD3, allophycocyanin-conjugated anti-CD56 and anti-CD69, PE-conjugated anti-NKp30, anti-NKp44, anti-NKp46, anti-CD16, anti DNAM-1, anti-CD94, anti-CD161, anti-CD56, anti-CD158a/h, anti-CD158b, anti-CD107a and anti-CD85j (BD PharMingen) or PE-conjugated anti-NKG2A (R&D Systems), for 30 min at 4°C. The NK-cell population was selected based on CD3 -CD56 + phenotype of gated lymphocytes according to forward-side scatter. For NK-cell ligand analysis, tumor cell lines were incubated with FITC-conjugated anti-HLA-ABC (BD PharMingen), or PE-conjugated anti-HLA-G, anti-HLA-E (Abcam), anti-CD112 and ant-MICA/B (BD PharMingen), anti-CD155 (BioLegend) or respective isotype controls. All samples were acquired on a BD FACS Calibur using Cellquest Pro software (BD Biosciences) and analyzed with FlowJo 7.6.2 software (Tree Star, Inc.).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!