HeLa and SiHa cells were plated in 6-well plates at 2 × 10 6 /well for 24h. After incubation with DFX (0-200 μM) for 24h, the cells were incubated in 70% cold alcohol overnight at 4 °C. Subsequently, a total of 1 × 10 6 cells were resuspended in 500 μL of PBS to which 100 μL RNAse A (Keygen Biotech, Nanjing, China) and 500 μL PI buffer were added. After incubation in darkness for 30 min at 37 °C, cell cycle phase distribution was assayed using a Beckman Coulter FC 500 type ow cytometer (Beckman Coulter, Miami, FL, USA) and the data were analyzed by multicycle AV for Windows software (Beckman Coulter, Miami, FL, USA). The results are indicated as mean values from three independent determinations.
Fc 500 type ow cytometer
Manufactured by Beckman Coulter
Sourced in United States
The Beckman Coulter FC 500 is a flow cytometer that measures the physical and chemical characteristics of particles, cells, or other biological entities suspended in a fluid. It utilizes laser technology to generate data on the size, granularity, and fluorescence intensity of the analyzed samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fc 500 type ow cytometer
1
Cell Cycle Analysis of HeLa and SiHa Cells
2
Cell Cycle Analysis of HeLa and SiHa Cells
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HeLa and SiHa cells were plated in 6-well plates at 2 × 10 6 /well for 24h. After incubation with DFX (0-200 μM) for 24h, the cells were incubated in 70% cold alcohol overnight at 4 °C. Subsequently, a total of 1 × 10 6 cells were resuspended in 500 μL of PBS to which 100 μL RNAse A (Keygen Biotech, Nanjing, China) and 500 μL PI buffer were added. After incubation in darkness for 30 min at 37 °C, cell cycle phase distribution was assayed using a Beckman Coulter FC 500 type ow cytometer (Beckman Coulter, Miami, FL, USA) and the data were analyzed by multicycle AV for Windows software (Beckman Coulter, Miami, FL, USA). The results are indicated as mean values from three independent determinations.
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