Ambion micropoly a puris kit

Sunitinib malate and triphenyl-tetrazolium chloride were purchased from Sigma Aldrich (UK) and dissolved in dimethyl sulphoxide (DMSO) and stored at -20 °C. Krebs perfusate salts were from VWR International (UK) or Fisher Scientific (UK). Ambion MicroPoly(A)Puris kit, Ambion mirVana miRNA Isolation Kit, Reverse Transcription Kit, Applied Biosystems MicroRNA Reverse Transcription Kit, TaqMan Universal master mix II (no UNG), MKK7 mRNA primers, Applied Biosystems primers assays (U6, rno-miR-1, hsa-miR-27a, hsa-miR-133a, and hsa-miR-133b) were purchased from Life Technologies (USA).
The iTaq Universal SYBR Green Supermix was purchased from BioRad (UK). Phospho-MKK7 (Ser271/Thr275), Total MKK7 rabbit mAb antibodies and anti-rabbit IgG, HRPlinked antibody and anti-biotin, HRP-linked antibody were purchased from Cell signaling technologies (UK).

Sunitinib malate and NQDI-1 were purchased from Sigma Aldrich (UK). Both drugs were 140 dissolved in dimethyl sulphoxide (DMSO) and stored at -20 °C. Krebs perfusate salts were 141 from either VWR International (UK) or Fisher Scientific (UK). Total ASK1 (Catalogue no 142 ab131506) was purchased from Abcam (UK). Phospho-ASK1 (Thr 845) (Catalogue no 143 3765S), Phospho-MKK7 (Ser271/Thr275) (Catalogue no 4171S), Total MKK7 (Catalogue no 144 4172S), Phospho-SAPK/JNK (Thr183/Tyr185) (Catalogue no 9251), Total SAPK/JNK rabbit 145 mAb antibody (Catalogue no 9252), anti-rabbit IgG, HRP-linked antibody and anti-biotin, 146 HRP-linked antibody were purchased from Cell signalling technologies (UK). All the 147 primary antibodies were from a rabbit host, and MKK7 and JNK were monoclonal antibodies, 148 whereas ASK1 was polyclonal (all antibodies were validated by the manufacturers). The 149 Ambion MicroPoly(A)Puris kit, Ambion mirVana miRNA Isolation Kit and Reverse 150 Transcription Kit were from Life Technologies (USA). The mRNA primers and the Applied 151 Biosystems primers assays (U6, rno-miR-1, hsa-miR-27a, hsa-miR-133a, and hsa-miR-152 133b) were purchased from Invitrogen (UK).The iTaq Universal SYBR Green Supermix was 153 purchased from BioRad (UK). The HL60 cell line were obtained from European Collection of 154
Cell Culture (UK) (catalogue no. 98070106). 155 156