We used Igor Pro 5.05A (Wavemetrics) for curve fitting and evoked PSC analysis and Graphpad (La Jolla, CA) for additional statistical analysis. To quantify evoked responses,15-20 traces were averaged and measured for a given condition in each cell.
Events characterized by a typical fast rise phase and exponential decay were selected for analysis. For all histological and electrophysiology data, normality was tested using the Shapiro-Wilk test to determine use of parametric or non-parametric statistical analysis. Data were compared using a Student's t test for parametric outcome measures, whereas a Mann-Whitney U test was used for non-parametric data. Data are expressed as mean ±SEM, with significance set at p<0.05. Sample sizes were selected to detect an effect size of 30-50% and a power of 0.8 with a set to p<0.05.
Events characterized by a typical fast rise phase and exponential decay were selected for analysis. For all histological and electrophysiology data, normality was tested using the Shapiro-Wilk test to determine use of parametric or non-parametric statistical analysis. Data were compared using a Student's t test for parametric outcome measures, whereas a Mann-Whitney U test was used for non-parametric data. Data are expressed as mean ±SEM, with significance set at p<0.05. Sample sizes were selected to detect an effect size of 30-50% and a power of 0.8 with a set to p<0.05.