Biotin conjugated anti mouse and rabbit secondary antibodies

Manufactured by GE Healthcare

Sourced in United Kingdom

Biotin conjugated anti-mouse and rabbit secondary antibodies are laboratory reagents designed to detect and visualize target proteins in various immunoassay techniques. These antibodies are conjugated with biotin, a small molecule that can be used to amplify the signal from the primary antibody-antigen interaction.

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Lab products found in correlation

Mouse monoclonal anti β actin antibody, by Merck Group (2 mentions) Anti mouse asc and caspase 1 antibodies, by Santa Cruz Biotechnology (2 mentions) Anti mouse and human il 1β antibodies, by R&D Systems (2 mentions) Nigericin, by InvivoGen (1 mentions) Seitu, by Merck Group (1 mentions) Acetaldehyde, by Thermo Fisher Scientific (1 mentions) Poly da dt, by InvivoGen (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Sodium orthovanadate, by Merck Group (1 mentions) Muscimol, by MP Biomedicals (1 mentions) Anti phosphotyrosine antibody, by Cell Signaling Technology (1 mentions) Adenosine triphosphate (atp), by Cytiva (1 mentions)

2 protocols using biotin conjugated anti mouse and rabbit secondary antibodies

Inflammasome Activation Pathway Protocol

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LPS, sodium orthovanadate, SEITU and monoclonal anti-mouse β-actin antibodies were from Sigma-Aldrich (St. Louis, MO). Anti-mouse ASC and caspase-1 antibodies were from Santa Cruz Biotechnology Inc. (Dallas, TX), and anti-mouse and human IL-1β antibodies were from R&D Systems (Minneapolis, MN). Biotin conjugated anti- mouse and rabbit secondary antibodies were from GE Healthcare UK Limited (Little Chalfont, Buckinghamshire, UK) and anti-goat IgG was from Jackson ImmunoResearch (West Grove, PA). For inflammasome stimulation, nigericin and poly (dA: dT) were from Invivogen (San Diego, CA), ATP and anthrax lethal factor and protective antigen were from Amersham Biosciences (Piscatawy, NJ) and BEI Resources (Manassas, VA), respectively. Ethanol and carboxy-PTIO were from Pharmco AAPER (Brookfield, CT) and Life Technologies Corporation (Carlsbad, CA). NAD⁺ was from Cayman Chamical (Ann Arbor, Michigan), and acetaldehyde and 4-methylpyrazole (4-MP) were from Acros Organics (Geel, Belgium). Dr. Michael P. Murphy (Medical Research Council Mitochondrial Biology Unit, University of Cambridge, Cambridge, UK) kindly provided MitoQ.

Hoyt L.R., Randall M.J., Ather J.L., DePuccio D.P., Landry C.C., Qian X., Janssen-Heininger Y.M., van der Vliet A., Dixon A.E., Amiel E, & Poynter M.E. (2017). Mitochondrial ROS induced by chronic ethanol exposure promote hyper-activation of the NLRP3 inflammasome. Redox Biology, 12, 883-896.

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Inflammasome Activation Reagents and Antibodies

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LPS, isoamyl alcohol, 2-methylbutane, picrotoxin, 3-(2-Carboxypiperazin-4-yl)propyl-1-phosphonic acid, sodium orthovanadate, and monoclonal anti-mouse β-actin antibodies were all purchased from Sigma-Aldrich (St. Louis, MO). Anti-mouse ASC and caspase-1 antibodies were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX), anti-phosphotyrosine antibodies from Cell Signaling (Danvers, MA), anti-mouse and human IL-1β antibodies from R&D Systems (Minneapolis, MN), and anti-human caspase-1 antibodies from BIOMOL International LP (Plymouth Meeting, PA). Recombinant mature mouse IL-1β was also purchased from R&D Systems. Biotin conjugated anti- mouse and rabbit secondary antibodies were from GE Healthcare UK Limited (Little Chalfont, Buckinghamshire, UK) and anti-goat IgG from Jackson ImmunoResearch (West Grove, PA). For inflammasome stimulation, ATP, nigericin, alum Imject, apoSAA, poly (dA:dT), and anthrax lethal factor and protective antigen were purchased from Amersham Biosciences (Piscatawy, NJ), Invivogen (San Diego, CA), Thermo Scientific (Waltham, MA), PeproTech Inc. (Rocky Hill, NJ), Invivogen, and BEI Resources (Manassas, VA), respectively. Muscimol was acquired from MP Biomedicals (Santa Ana, CA), and ethanol from Pharmco AAPER (Brookfield, CT). ASC (Tyr144) phospho-specific antibody was purchased from ECM Biosciences (Versailles, KY).

Hoyt L.R., Ather J.L., Randall M.J., DePuccio D.P., Landry C.C., Wewers M.D., Gavrilin M.A, & Poynter M.E. (2016). Ethanol and Other Short-Chain Alcohols Inhibit NLRP3 Inflammasome Activation through Protein Tyrosine Phosphatase Stimulation. Journal of immunology (Baltimore, Md. : 1950), 197(4), 1322-1334.

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