Streptococcus oralis atcc 9811

Streptococcus oralis (ATCC^® 9811™) was purchased from the American Type Culture Collection (ATCC, Manassas, USA). Actinomyces naeslundii (DSM 43013), Veillonella dispar (DSM 20735) and Porphyromonas gingivalis (DSM 20709) were acquired from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany). Bacteria were stored at -80°C and routinely cultivated in brain heart infusion medium (BHI; Oxoid, Wesel, Germany), supplemented with 10 μg/ml vitamin K (Roth, Karlsruhe, Germany) under anaerobic conditions (80% N₂, 10% H₂, 10% CO₂) at 37°C prior to experiments.

Streptococcus oralis ATCC® 9811 was obtained from the American Type Culture Collection (ATCC®, Manassas, VA, USA). Actinomyces naeslundii DSM 43013 and Porphyromonas gingivalis DSM 20709 were obtained from the German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany). Aggregatibacter actinomycetemcomitans MCCM 2474 strain was obtained from the Microbial Culture Collection Marburg.
All bacterial strains were routinely stored as glycerol stocks at −80 °C. As culture medium Todd-Hewitt Broth (Oxoid Limited, Hampshire, UK) supplemented with 10% yeast extract (THBy, Carl Roth GmbH + Co. KG, Karlsruhe, Germany), Brain–Heart Infusion (BHI, Oxoid Limited) supplemented with 10 μg mL⁻¹ vitamin K (Oxoid Limited) and Fastidious Anaerobe Broth (FAB, Oxoid Limited) were used as specified in Table 1. For the bacteria culture, 20 μL (S. oralis, A. naeslundii, Aac) or 30 μl (P. gingivalis) of the glycerol stocks were added into 10 mL of the corresponding medium in a 50 mL centrifugal tube and incubated under the conditions shown in Table 1. Then, bacterial cells were washed three times with autoclaved deionized water by centrifuging at 2000 RCF for 5 min.