Plan neofluar 40 1.3 oil

For fluorescent recordings an advanced wide-field fluorescent microscope (Till Photonics, Graefling, Germany) equipped with a motorized sample stage, a polychrome V (Till Photonics), a 40x objective (alpha Plan Fluar 40x, Zeiss, Göttingen, Germany) and a charge-coupled device camera (AVT Stingray F145B, Allied Vision Technologies, Stadtroda, Germany) was used. The FRET-based mitochondrial Ca²⁺ sensor 4mtD3cpV was excited at 430 nm and emission was collected using the dichrotome dual emission filter set (dichroic 535dcxr, CFP emitter 482/18 nm and YFP emitter 535/3 nm). TMRM was visualized at an excitation of 550 nm and emission of 575 nm. For monitoring NO^• dynamics G-geNOp expressing cells were exposed to 480 nm and emitted at 515 nm. For simultaneous measurements of cytosolic Ca²⁺and NO^•, fura-2/am loaded and G-geNOp expressing cells were alternately excited at 340, 380 and 480 nm, respectively and emission was recorded at 515 nm (515dcxr). Data acquisition and control was carried out by the Live Acquisition 2.0.0.12 software (Till Photonics). Confocal imaging of adenoviral infected G-geNOp expressing EA.hy926 or transient transfected eNOS-RFP expressing HEK293 cells was performed as described previously by using a 40× objective (Plan-Neofluar 40×/1.3 oil, Zeiss) or a 100× objective (Plan-Fluar 100×/1.45 oil, Zeiss), respectively [17 (link)].