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Foetal bovine serum (fbs)

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Foetal bovine serum (FBS) is a cell culture media supplement derived from the blood of bovine foetuses. It provides a complex mixture of proteins, growth factors, and other components that support the growth and proliferation of cells in vitro.

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Lab products found in correlation

Nupage novex 10 bis tris gel, by Thermo Fisher Scientific (2 mentions) Anti p erk, by Cell Signaling Technology (2 mentions) Streptomycin, by Merck Group (2 mentions) Penicillin, by Merck Group (2 mentions) Dmem medium, by Merck Group (2 mentions) Anti gapdh, by Novus Biologicals (2 mentions) Anti erk1 2, by Cell Signaling Technology (2 mentions) Anti prsk, by Abcam (2 mentions) Anti pets1, by Immunoway (2 mentions)

2 protocols using foetal bovine serum (fbs)

1

Investigating ERK1/2 Signaling Dynamics

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Human MDA-MB-231 breast cancer cells were cultivated in monolayers in DMEM medium (Sigma Aldrich) supplemented with 10% foetal bovine serum (Clontech), penicillin and streptomycin (Sigma Aldrich). ERK1/2 inhibitors SCH772984 and VTX-11e were added at 100 nM final concentration for 4 hours, followed by two washes in PBS and release into fresh media for 0.5, 1 and 2 hours. Cells were harvested by trypsinization, washed once with cold PBS, re-suspended in SDS-PAGE loading buffer and sonicated. Equal amounts of protein were analysed by gel electrophoresis followed by Western blotting. NuPAGE-Novex 10% Bis-Tris gels (Invitrogen) were run according to manufacturer’s instructions. The following antibodies were used in immunoblotting: anti-pERK (Cell Signaling Technology), anti-pRSK (Abcam) and anti-pETS1 (Immunoway). Anti-ERK1/2 (Cell Signaling Technology), anti-GAPDH (Novus Biologicals) or anti-α-tubulin (Cancer Research UK Monoclonal Antibody Service) antibodies were used as loading controls.
Chaikuad A., Tacconi E., Zimmer J., Liang Y., Gray N.S., Tarsounas M, & Knapp S. (2014). A unique inhibitor binding site in ERK1/2 is associated with slow binding kinetics. Nature chemical biology, 10(10), 853-860.
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2

Investigating ERK1/2 Signaling Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human MDA-MB-231 breast cancer cells were cultivated in monolayers in DMEM medium (Sigma Aldrich) supplemented with 10% foetal bovine serum (Clontech), penicillin and streptomycin (Sigma Aldrich). ERK1/2 inhibitors SCH772984 and VTX-11e were added at 100 nM final concentration for 4 hours, followed by two washes in PBS and release into fresh media for 0.5, 1 and 2 hours. Cells were harvested by trypsinization, washed once with cold PBS, re-suspended in SDS-PAGE loading buffer and sonicated. Equal amounts of protein were analysed by gel electrophoresis followed by Western blotting. NuPAGE-Novex 10% Bis-Tris gels (Invitrogen) were run according to manufacturer’s instructions. The following antibodies were used in immunoblotting: anti-pERK (Cell Signaling Technology), anti-pRSK (Abcam) and anti-pETS1 (Immunoway). Anti-ERK1/2 (Cell Signaling Technology), anti-GAPDH (Novus Biologicals) or anti-α-tubulin (Cancer Research UK Monoclonal Antibody Service) antibodies were used as loading controls.
Chaikuad A., Tacconi E., Zimmer J., Liang Y., Gray N.S., Tarsounas M, & Knapp S. (2014). A unique inhibitor binding site in ERK1/2 is associated with slow binding kinetics. Nature chemical biology, 10(10), 853-860.
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