Medium DMEM was purchased from Corning. Fetal bovine serum (FBS) and pancreatin were purchased from GiBico. N-nitrosodiethylamine12 (link) and N-nitrosomorpholine (NMOR) were purchased from Sigma (St, Louis, MO, USA). Matrigel was purchased from BD. Rabbit polyclonal antibody to AFP (14550-1-AP), Rabbit polyclonal antibody to CK7 (15539-1-AP), Rabbit polyclonal antibody to CK8 (10384-1-AP), Mouse monoclonal to beta actin (60008-1-lg) and all secondary antibodies were procured from proteinteck (Proteinteck, CHINA).
N nitrosomorpholine
Manufactured by Merck Group
Sourced in United States
N-nitrosomorpholine is a chemical compound used as a laboratory reagent. It is a clear, colorless liquid with a characteristic odor. The compound is soluble in organic solvents and is commonly used in analytical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
N nitrosodiethylamine, by Merck Group (2 mentions)
N nitrosodi n propylamine, by Merck Group (2 mentions)
N nitrosopyrrolidine, by Merck Group (2 mentions)
N nitrosopiperidine, by Merck Group (2 mentions)
N nitrosodiphenylamine, by Merck Group (2 mentions)
N nitrosodimethylamine, by Merck Group (2 mentions)
Matrigel, by BD (1 mentions)
Diethylamine, by Merck Group (1 mentions)
Pyrrolidine, by Merck Group (1 mentions)
Sodium nitrite, by Merck Group (1 mentions)
N nitrosodibutylamine, by Merck Group (1 mentions)
N nitrosomethylethylamine, by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Acetaldehyde, by Merck Group (1 mentions)
Sulfamic acid, by Merck Group (1 mentions)
Dimethylamine, by Merck Group (1 mentions)
N nitroso n methylethylamine, by Merck Group (1 mentions)
Hplc grade methanol, by Duksan Pure Chemicals (1 mentions)
3 protocols using n nitrosomorpholine
1
Hepatocellular Carcinoma Cell Culture
2
Analysis of N-Nitrosamines in Chemicals
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dimethylamine (DMA), diethylamine
(DEA), sulfamic acid, formaldehyde (HCHO, 37 wt % in H2O containing 10–15% methanol as a stabilizer), acetone, and
benzaldehyde were obtained from Sigma-Aldrich, pyrrolidine (PYR),
morpholine (MOR), sodium nitrite, and acetaldehyde from Merck, and N-methylethylamine (MEA) from Tokyo Chemical Industry (TCI)
Co., Ltd. The US-EPA 8270 N-nitrosamines mix (2000
μg/mL each component in methanol), containing N-nitrosodibutylamine (NDBA), N-nitrosodiethylamine
(NDEA), N-nitrosoDimethylamine (NDMA), N-nitrosodiphenylamine (NDPhA), N-nitrosodi-n-propylamine
(NDPA), N-nitrosomethylethylamine (NMEA), N-nitrosomorpholine (NMOR), N-nitrosopiperidine
(NPIP), and N-nitrosopyrrolidine (NPYR), was obtained
from Sigma-Aldrich. All chemicals were of analytical grade and used
as received without further purification.
(DEA), sulfamic acid, formaldehyde (HCHO, 37 wt % in H2O containing 10–15% methanol as a stabilizer), acetone, and
benzaldehyde were obtained from Sigma-Aldrich, pyrrolidine (PYR),
morpholine (MOR), sodium nitrite, and acetaldehyde from Merck, and N-methylethylamine (MEA) from Tokyo Chemical Industry (TCI)
Co., Ltd. The US-EPA 8270 N-nitrosamines mix (2000
μg/mL each component in methanol), containing N-nitrosodibutylamine (NDBA), N-nitrosodiethylamine
(NDEA), N-nitrosoDimethylamine (NDMA), N-nitrosodiphenylamine (NDPhA), N-nitrosodi-n-propylamine
(NDPA), N-nitrosomethylethylamine (NMEA), N-nitrosomorpholine (NMOR), N-nitrosopiperidine
(NPIP), and N-nitrosopyrrolidine (NPYR), was obtained
from Sigma-Aldrich. All chemicals were of analytical grade and used
as received without further purification.
3
Quantification of Nitrosamines in Chemical Samples
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N-nitrosodimethylamine (NDMA), n-nitrosodiethylamine (NDEA), n-nitrosodi-n-propylamine (NDPA), n-nitrosodi-n-buthylamine (NDBA), n-nitrosopiperidine (NPIP), n-nitrosopyrrolidine (NPYR), n-nitrosomorpholine (NMOR), n-nitrosodiphenylamine (NDPhA), n-nitroso-n-methylethylamine (NMEA), and n-nitrodi-n-propylamine-d14 (internal standard) were purchased from Sigma-Aldrich Korea (Cheoin-gu, Yongin, Korea). Methanol (HPLC grade), ethanol (HPLC grade), acetonitrile (HPLC grade), acetic acid, and n-heptane were purchased from Duksan Pure Chemicals Co. (Danwon-gu, Ansan, Korea). Ultra-pure water was prepared using an aquaMAXTM Ultra 370 series (YL Instruments, Anyang, Korea) water purification system (18.2 MΩ cm).
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