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A1r meta confocal microscope

Manufactured by Nikon

The Nikon A1R Meta confocal microscope is a high-performance imaging system designed for advanced fluorescence microscopy applications. It features a fast and sensitive resonant scanner, providing rapid image acquisition and enabling live-cell imaging. The system incorporates a variety of laser options, allowing for the excitation of a wide range of fluorophores. The A1R Meta's modular design allows for customization to suit the specific needs of the user's research.

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2 protocols using a1r meta confocal microscope

1

Immunofluorescence Staining of Kidney Biopsies

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Kidney biopsies and 293 T cells fixed in neutral buffered formalin were embedded in paraffin or optimal cutting temperature compound by using standard procedures. Frozen and paraffin sections were stained with immunofluorescence, respectively. Immunofluorescent staining and images were obtained by a Nikon A1R Meta confocal microscope. Cover slips were observed.
The antibodies used were list below: anti-Cubilin-C-terminal antibody (1:500, ab191073, Abcam), Rat Cubilin(CUBN) polyclonal antibody (1:100, 31010, Bicell Scientific), anti-Synaptop-odin antibody (1:50, 21064-1-AP, Proteintech), anti-Wilms Tumor Protein antibody (1:50, ab89901, Abcam), anti-COL4A3 antibody (1:100, Kingmed, Guangzhou, China), anti-COL4A5 antibody (1:100, Kingmed, Guangzhou, China), anti-Amnionless antibody (1:10, sc-365384, Santa Cruz), anti-Megalin Antibody (1:30, CD7D5, Novus Biologicals), goat polyclonal secondary antibody to mouse Alexa fluor 488 (1:400, ab150113, Abcam), goat polyclonal secondary antibody to rabbit Alexa fluor 555 (1:400, ab150078, Abcam), rabbit monoclonal to HA tag (1:500, ab236632, Abcam), goat polyclonal secondary antibody to rabbit Alexa fluor 647 (1: 400, ab150079, Abcam), goat anti-mouse Alexa fluor 568 (1: 400, ab175473, Abcam), DAPI (1:1000, C1002, Beyotime).
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2

Immunofluorescent Staining of Kidney Biopsies

Check if the same lab product or an alternative is used in the 5 most similar protocols
Kidney biopsies and 293T cells fixed in neutral buffered formalin were embedded in paraffin or optimal cutting temperature compound by using standard procedures (LeBleu et al., 2013 ). Frozen and paraffin sections were stained with immunofluorescence, respectively. Immunofluorescent staining and images were obtained by a Nikon A1R Meta confocal microscope. Coverslips were observed.
The antibodies used were listed below: rabbit polyclonal antibody to Synaptopodin (1:50, 21,064‐1‐AP, Proteintech), rabbit monoclonal antibody to Cubilin (1:500, ab191073, Abcam), rabbit polyclonal antibody Megalin antibody (1:30, CD7D5, Novus Biologicals), anti‐COL4A3 antibody (1:100, Kingmed), anti‐COL4A4 antibody (1:100, Kingmed), anti‐COL4A5 antibody (1:100, Kingmed), goat polyclonal secondary antibody to mouse Alexa fluor 488 (1:400, ab150113, Abcam), goat polyclonal secondary antibody to rabbit Alexa fluor 555 (1:400, ab150078, Abcam).
Apoptosis index was detected by One‐step TdT‐mediated dUTP nick‐end labeling (TUNEL) Assay Kit (E‐CK‐A325, Elabscience).
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