The antibodies used were list below: anti-Cubilin-C-terminal antibody (1:500, ab191073, Abcam), Rat Cubilin(CUBN) polyclonal antibody (1:100, 31010, Bicell Scientific), anti-Synaptop-odin antibody (1:50, 21064-1-AP, Proteintech), anti-Wilms Tumor Protein antibody (1:50, ab89901, Abcam), anti-COL4A3 antibody (1:100, Kingmed, Guangzhou, China), anti-COL4A5 antibody (1:100, Kingmed, Guangzhou, China), anti-Amnionless antibody (1:10, sc-365384, Santa Cruz), anti-Megalin Antibody (1:30, CD7D5, Novus Biologicals), goat polyclonal secondary antibody to mouse Alexa fluor 488 (1:400, ab150113, Abcam), goat polyclonal secondary antibody to rabbit Alexa fluor 555 (1:400, ab150078, Abcam), rabbit monoclonal to HA tag (1:500, ab236632, Abcam), goat polyclonal secondary antibody to rabbit Alexa fluor 647 (1: 400, ab150079, Abcam), goat anti-mouse Alexa fluor 568 (1: 400, ab175473, Abcam), DAPI (1:1000, C1002, Beyotime).
A1r meta confocal microscope
The Nikon A1R Meta confocal microscope is a high-performance imaging system designed for advanced fluorescence microscopy applications. It features a fast and sensitive resonant scanner, providing rapid image acquisition and enabling live-cell imaging. The system incorporates a variety of laser options, allowing for the excitation of a wide range of fluorophores. The A1R Meta's modular design allows for customization to suit the specific needs of the user's research.
Lab products found in correlation
2 protocols using a1r meta confocal microscope
Immunofluorescence Staining of Kidney Biopsies
The antibodies used were list below: anti-Cubilin-C-terminal antibody (1:500, ab191073, Abcam), Rat Cubilin(CUBN) polyclonal antibody (1:100, 31010, Bicell Scientific), anti-Synaptop-odin antibody (1:50, 21064-1-AP, Proteintech), anti-Wilms Tumor Protein antibody (1:50, ab89901, Abcam), anti-COL4A3 antibody (1:100, Kingmed, Guangzhou, China), anti-COL4A5 antibody (1:100, Kingmed, Guangzhou, China), anti-Amnionless antibody (1:10, sc-365384, Santa Cruz), anti-Megalin Antibody (1:30, CD7D5, Novus Biologicals), goat polyclonal secondary antibody to mouse Alexa fluor 488 (1:400, ab150113, Abcam), goat polyclonal secondary antibody to rabbit Alexa fluor 555 (1:400, ab150078, Abcam), rabbit monoclonal to HA tag (1:500, ab236632, Abcam), goat polyclonal secondary antibody to rabbit Alexa fluor 647 (1: 400, ab150079, Abcam), goat anti-mouse Alexa fluor 568 (1: 400, ab175473, Abcam), DAPI (1:1000, C1002, Beyotime).
Immunofluorescent Staining of Kidney Biopsies
The antibodies used were listed below: rabbit polyclonal antibody to Synaptopodin (1:50, 21,064‐1‐AP, Proteintech), rabbit monoclonal antibody to Cubilin (1:500, ab191073, Abcam), rabbit polyclonal antibody Megalin antibody (1:30, CD7D5, Novus Biologicals), anti‐COL4A3 antibody (1:100, Kingmed), anti‐COL4A4 antibody (1:100, Kingmed), anti‐COL4A5 antibody (1:100, Kingmed), goat polyclonal secondary antibody to mouse Alexa fluor 488 (1:400, ab150113, Abcam), goat polyclonal secondary antibody to rabbit Alexa fluor 555 (1:400, ab150078, Abcam).
Apoptosis index was detected by One‐step TdT‐mediated dUTP nick‐end labeling (TUNEL) Assay Kit (E‐CK‐A325, Elabscience).
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