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Phenazine methosulphate

Manufactured by Tokyo Chemical Industry
Sourced in Japan

Phenazine methosulphate is a chemical compound used as a laboratory reagent. It is commonly employed as an electron acceptor in various biochemical assays and experiments.

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5 protocols using phenazine methosulphate

1

Comprehensive Reagents and Procedures

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Folin–Ciocalteau’s reagent, bovine serum albumin, ABTS, acarbose, sodium dodecyl sulfate, EDTA, sodium bicarbonate, α-glucosidase, iNOS, TNF-α, IL-6, acetylcholinesterase, lipopolysaccharide, acetylcholine iodide, DTNB, TPTZ, chlorogenic acid, DMSO, and Trolox were purchased from Sigma-Aldrich (St. Louis, MO, USA). Glycine was purchased from J.T.Baker (Phillipsburg, NJ, USA). DPPH, phenazine methosulphate, and nitroblue tetrazolium were supplied from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Thiazolyl blue tetrazolium bromide was purchased from MedChemExpress (Monmouth Junction, NJ, USA). Butyl hydroxytoluene, nicotinamide adenine dinucleotide, sodium acetate, and potassium acetate were supplied from Acros Organics (Geel, Belgium). Ammonium persulfate and TEMED were purchased from Bio-Rad (Hercules, CA, USA). Tween20 was purchased from Merck (Darmstadt, Germany). Acetic acid was supplied from Macron Fine Chemicals (Center Valley, PA, USA). Iron (III) chloride, aluminum chloride, as well as p-Nitro-phenyl-α-D-glucopyranoside were purchased from Alfa Aesar (Lancashire, UK). Disodium hydrogenphosphate, sodium carbonate, potassium peroxodisulfate, and potassium dihydrogenphosphate were obtained from SHOWA Chemical Co. (Sun Valley, CA, USA).
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2

Quercetin Bioactivity Evaluation Protocol

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Quercetin was purchased from MedChemExpress (Monmouth Junction, NJ, USA). 2,2’-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Folin–Ciocalteu reagent, tyrosinase, gallic acid, α-glucosidase, 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), bovine serum albumin, and Trolox were purchased from Sigma-Aldrich (St. Louis, MO, USA). Disodium hydrogen phosphate, potassium peroxodisulfate, sodium carbonate, and sodium dihydrogen phosphate were purchased from SHOWA Chemical Co. Ltd. (Chuo-ku, Japan). Ferric chloride (FeCl3), p-nitro-phenyl-α-D-glucopyranoside (p-NPG), and aluminum chloride (AlCl3) were obtained from Alfa Aesar (Lancashire, UK). 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), nitroblue tetrazolium (NBT), and phenazine methosulphate (PMS) were supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Potassium acetate, acarbose, sodium acetate, nicotinamide adenine dinucleotide (NADH), and butyl hydroxytoluene (BHT) were acquired from Acros Organics (Geel, Belgium). Arbutin was purchased from AK Scientific. Acetic acid was supplied by Avantor Performance Materials, LLC. (Radnor, PA, USA).
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3

Antioxidant and Enzyme Inhibition Assays

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Gallic acid, Folin–Ciocalteu reagent, hydrogen peroxide solution, α-glucosidase, acetylthiocholine iodide, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetylcholinesterase, 5,5′-dithio-bis(2-nitrobenzoic) acid, 2,4,6-tripyridyl-s-triazine, and Trolox were bought from Sigma-Aldrich. Nitroblue tetrazolium, 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl, loroglucinol, and phenazine methosulphate were supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Aluminum chloride, ferric chloride, and p-nitrophenyl-α-D-glucopyranoside were bought from Alfa Aesar. Potassium dihydrogenphosphate, sodium carbonate, potassium peroxodisulfate, disodium hydrogenphosphate, and sodium carbonate were bought from the Showa Chemical Industry Co., Ltd. (Tokyo, Japan). Sodium acetate, butyl hydroxytoluene, potassium acetate, and nicotinamide adenine dinucleotide were supplied by Acros Organics. Galanthamine hydrobromide was bought from MedchemExpress.
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4

Antioxidant and enzyme inhibitory assays

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Folin-ciocalteau’s reagent, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (abts), nicotinamide adenine dinucleotide (NADH), ascorbic acid, ethylenediaminetetraacetic acid (EDTA), α-glucosidase, and hydrogen peroxide solution were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ferric chloride (FeCl3) and p-nitro-phenyl-α-d-glucopyranoside (p-NPG) were supplied by Alfa Aesar (Lancashire, UK). Potassium peroxodisulfate, sodium carbonate, potassium dihydrogenphosphate, and disodium hydrogenphosphate were purchased from the SHOWA Chemical Co. Ltd. (Chuo-ku, Japan). Loroglucinol, 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), phenazine methosulphate (PMS), 2-thiobarbituric acid (TBA), nitroblue tetrazolium (NBT), and deoxyribose were obtained from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Butyl hydroxytoluene (BHT), nicotinamide adenine dinucleotide (NADH), and trichloroacetic acid (TCA) were purchased from Acros Organics (Geel, Belgium).
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5

Antioxidant Capacity Evaluation Protocol

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Ascorbic acid, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), α-glucosidase, ethylenediaminetetraacetic acid (EDTA), gallic acid, Folin-ciocalteau’s reagent, hydrogen peroxide solution, Trolox, and 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ferric chloride (FeCl3) and p-nitro-phenyl-α-D-glucopyranoside (p-NPG) were obtained from Alfa Aesar (Lancashire, UK). Disodium hydrogenphosphate, potassium peroxodisulfate, sodium carbonate, and potassium dihydrogenphosphate were acquired from the SHOWA Chemical Co. Ltd. (Chuo-ku, Japan). 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), deoxyribose, nitroblue tetrazolium (NBT), phenazine methosulphate (PMS), and 2-thiobarbituric acid (TBA) were supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Butyl hydroxytoluene (BHT), acarbose, nicotinamide adenine dinucleotide (NADH), sodium acetate, trichloroacetic acid (TCA), and potassium acetate were acquired from Acros Organics (Geel, Belgium). Acetic acid was supplied by Avantor Performance Materials, LLC (Radnor, PA, USA). Sodium hydroxide solution was acquired from Merck (Darmstadt, Germany).
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