Jurkat T cells were stimulated under indicated conditions. About half a million cells per condition were harvested, washed and incubated with fluorophore-conjugated antibodies for 20 min at room temperature in MACS buffer (DBPS without calcium and magnesium, supplemented with 1% FBS). Cells were washed and resuspended in MACS buffer and analyzed by flow cytometry in corresponding fluorescent channels relative to unstained control. Antibodies used: anti-CD69 PE, anti-CD28 APC, anti-CD3 BV711, anti-CD45RO BV711, anti-CXCR3 FITC, anti-CCR4 BV421, anti-CCR6 BV605, and/or anti-CCR7 BV650 antibodies (all diluted 1:100 and from Biolegend).
Anti ccr4 bv421
Manufactured by BioLegend
The Anti-CCR4 BV421 is a fluorescence-conjugated antibody that binds to the C-C Chemokine Receptor Type 4 (CCR4) on the surface of cells. CCR4 is a G protein-coupled receptor involved in the trafficking and migration of certain types of T cells. The BV421 fluorescent dye conjugated to the antibody allows for the detection and analysis of CCR4-expressing cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti ccr4 bv421
1
Jurkat T Cell Phenotyping by Flow
2
Jurkat T Cell Phenotyping by Flow
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