A 6044

Manufactured by Active Motif

The A-6044 is a lab instrument designed for DNA extraction and purification. It utilizes a magnetic bead-based method to isolate high-quality DNA from a variety of sample types. The core function of the A-6044 is to automate the DNA extraction process, providing consistent and reliable results.

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Lab products found in correlation

Dowex 1x2 400 ion exchange resin, by Merck Group (1 mentions)

2 protocols using a 6044

Myc-driven Lymphoma Cell Death Assay

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Cell lines were derived from frozen single cell suspensions of lymphoma-burdened spleens taken from Eµ-Myc^T/+;Bcl-w^+/+ (wild-type), Eµ-Myc^T/+;Bcl-w^+/− (heterozygous), and Eµ-Myc^T/+;Bcl-w^−/− (knockout) mice at ethical endpoint (as described in the supplementary methods). Cell death was measured 24 h after in vitro treatment with S63845 (MCL-1 inhibitor; Active Biochem #A-6044) at 0/1/8/40/100/500 nM or ABT-737 (BCL-2 + BCL-XL + BCL-W inhibitor; Active Biochem #A-1002) at 0/1/200/1000/5000 nM. See supplementary materials for additional information.

Diepstraten S.T., La Marca J.E., Chang C., Young S., Strasser A, & Kelly G.L. (2023). BCL-W makes only minor contributions to MYC-driven lymphoma development. Oncogene, 42(37), 2776-2781.

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Fatty Acid Oxidation Assay in MEFs

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MEFs (10⁵/well) were plated in six-well plates, allowed to adhere overnight, and then pre-incubated in either serum-containing or serum-free medium for 2 hours. Cells were then changed to medium containing 1μCi [9,10(n)-³H]palmitic acid (Perkin-Elmer, Cat# NET043001MC) or 1.8 μCi n-[5,6-³H]hexanoic acid (American Radiolabeled Chemicals Inc., Cat# ART 1754-250) and 1 mM carnitine for 2 hours, maintaining serum-containing or serum-free conditions. The medium was collected and eluted in columns packed with DOWEX 1X2-400 ion exchange resin (Sigma-Aldrich, Cat# 217395) to analyze the released ³H₂O. Counts per min (CPM) were normalized to protein content in cell lysates. For cells treated with S63845 (Active Biochem, Cat# A-6044), the compound was added to the cells with [9,10(n)-³H]palmitic acid for two hours at a final sub-cytotoxic concentration of 1 μM. For tamoxifen-induced deletion of Mcl-1, cells were treated with tamoxifen or vehicle for 48 hours and allowed to recover for an additional 48 hours prior to conducting the experiment.

Escudero S., Zaganjor E., Lee S., Mill C.P., Morgan A.M., Crawford E.B., Chen J., Wales T.E., Mourtada R., Luccarelli J., Bird G.H., Steidl U., Engen J.R., Haigis M.C., Opferman J.T, & Walensky L.D. (2018). Dynamic Regulation of Long-Chain Fatty Acid Oxidation by a Noncanonical Interaction between the MCL-1 BH3 Helix and VLCAD. Molecular cell, 69(5), 729-743.e7.

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