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Hygromycin stock solution

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Hygromycin stock solution is a concentrated liquid containing the antibiotic hygromycin B. It is used for the selection and maintenance of hygromycin-resistant cells in cell culture applications.

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Lab products found in correlation

Doxycycline, by Merck Group (2 mentions) Puromycin, by Thermo Fisher Scientific (2 mentions) Shp099, by Novartis (1 mentions) 4 oht, by Merck Group (1 mentions) Erlotinib, by MedChemExpress (1 mentions) Dabrafenib, by GlaxoSmithKline (1 mentions) Vemurafenib, by Plexxikon (1 mentions) Trametinib, by GlaxoSmithKline (1 mentions) Plx8394, by Plexxikon (1 mentions) Plx7904, by Plexxikon (1 mentions) Lgx818, by Novartis (1 mentions) Ly3009120, by Active Motif (1 mentions) Lapatinib, by GlaxoSmithKline (1 mentions)

2 protocols using hygromycin stock solution

1

Sourcing and Preparing Experimental Reagents

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erlotinib was obtained from MedChem Express. osimertinib was obtained from SelleckChem Express. SHP099 was provided by Novartis. Doxycycline and 4-OHT were from Sigma Aldrich. Puromycin and hygromycin stock solution were from Invitrogen. Drugs were dissolved in DMSO to yield 10mM stock and store at −20°C.
Su W., Mukherjee R., Yaeger R., Son J., Xu J., Na N., Timaul N.M., Hechtman J., Paroder V., Lin M., Mattar M., Qiu J., Chang Q., Zhao H., Zhang J., Little M., Adachi Y., Han S.W., Taylor B.S., Ebi H., Abdel-Wahab O., de Stanchina E., Rudin C.M., Jänne P.A., McCormick F., Yao Z, & Rosen N. (2022). ARAF protein kinase activates RAS by antagonizing its binding to the RASGAP NF1. Molecular cell, 82(13), 2443-2457.e7.
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2

Multi-Kinase Inhibitor Screening Protocol

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BGB283 and BGB659 were obtained from Beigene. vemurafenib, PLX7904, PLX8394 from Plexxikon. lapatinib, trametinib, dabrafenib from GlaxoSmithKline. LGX818 is from Novartis. LY3009120 purchased from Active Biochem, Doxycycline from Sigma Aldrich, Puromycin, Hygromycin stock solution from Invitrogene., other drugs from Selleckchem. Drugs were dissolved in DMSO to yield 10mM stock and stored at −20°C.
Yao Z., Torres N.M., Tao A., Gao Y., Luo L., Li Q., de Stanchina E., Abdel-Wahab O., Solit D.B., Poulikakos P, & Rosen N. (2015). BRAF mutants evade ERK dependent feedback by different mechanisms that determine their sensitivity to pharmacologic inhibition. Cancer cell, 28(3), 370-383.
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