The antibodies used in this study are as follows: anti-mouse CD30 (clone: mCD30.1) (BD Biosciences, San Jose, CA, USA), anti-mouse CD8α (clone: 53-6.7) (eBioscience, San Diego, CA, USA; BioLegend, San Diego, CA, USA), anti-mouse CD4 (clone: GK1.5) (eBioscience, BioLegend), anti-mouse CD3ε (clone: 145-2C11) (eBioscience, BioLegend), anti-mouse CD44 (clone: IM7) (eBioscience), anti-mouse T-bet (clone: 4B10) (eBioscience), anti-mouse Foxp3 (clone: FJK-16s) (eBioscience), anti-mouse Bcl6 (clone: BCL-DWN) (eBioscience), anti-mouse PD-1 (clone: J43) (eBioscience), anti-mouse CXCR5 (clone:SPRCL5) (eBioscience), anti-mouse CD62L (clone: MEL-14) (eBioscience), anti-mouse IFNγ (clone: XMG1.2) (eBioscience), anti-mouse Tim-3 (clone: RMT3-23) (eBioscience), and anti-mouse CD25 (clone: PC61.5) (eBioscience).
Anti mouse cd8α
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-mouse CD8α is a monoclonal antibody that specifically binds to the CD8α subunit of the CD8 co-receptor expressed on the surface of mouse cytotoxic T cells and a subset of natural killer cells. It is used for the identification and characterization of these cell populations in flow cytometry and other immunological applications.
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Lab products found in correlation
Anti mouse cd4, by Thermo Fisher Scientific (1 mentions)
Anti mouse pd 1, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd25, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd62l, by Thermo Fisher Scientific (1 mentions)
Anti mouse ifn γ, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd3ε, by Thermo Fisher Scientific (1 mentions)
Immpact dab peroxidase, by Vector Laboratories (1 mentions)
2 protocols using anti mouse cd8α
1
Antibody Panel for Immune Profiling
2
CD8+ Tumor-Infiltrating Lymphocyte Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Select tumor samples were collected via blunt dissection of footpad tissue, immersion-fixed in 10% neutral buffered formalin for 12–24 h, processed routinely, and embedded in paraffin. Briefly, 4 μm sections were collected on Plus-charged slides, and immunohistochemistry was performed on a Dako Autostainer-Plus Slide Stainer using an anti-Mouse CD8α (Clone 4SM15, eBioscience) at 1:5000 dilution. Detection was performed using goat anti-rat (Jackson Immuno) at 2 μg/mL, followed by Vectastain Elite ABC HRP Standard Peroxidase Kit (Vector Laboratories), visualized with ImmPACT DAB Peroxidase (Vector Laboratories), and counterstained with Gills #2 Hematoxylin (Richard-Allen Scientific). Rat IgG2aĸ (BD Pharmingen) at 1:5000 served as isotype control. Rat spleen was used as the positive control tissue. All samples were examined by a board-certified pathologist for detection of CD8+ cells within the tumor center.
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