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Foxo1

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FoxO1 is a protein that functions as a transcription factor. It plays a role in the regulation of gene expression related to cellular processes such as cell cycle, apoptosis, and metabolism.

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Lab products found in correlation

P akt ser473, by Thermo Fisher Scientific (2 mentions) Ps6rp, by Cell Signaling Technology (2 mentions) Apotag plus peroxidase in situ tunel apoptosis kit, by Merck Group (2 mentions) P erk, by Cell Signaling Technology (2 mentions) Nanodrop, by Thermo Fisher Scientific (2 mentions) Humanht 12 v4 expression beadchip kit, by Illumina (1 mentions) Mirneasy mini kit, by Qiagen (1 mentions) Mcl 1, by Santa Cruz Biotechnology (1 mentions) Akt and p akt, by Cell Signaling Technology (1 mentions) Plko bim shrna, by Thermo Fisher Scientific (1 mentions) Foxo1, by Cell Signaling Technology (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Bcl 2, by BD (1 mentions) Csf 1, by Thermo Fisher Scientific (1 mentions) Cxcr4, by Thermo Fisher Scientific (1 mentions) Mmp14, by Thermo Fisher Scientific (1 mentions) Viia 7 real time pcr system, by Thermo Fisher Scientific (1 mentions) Rpl13a, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Foxo1 siRNA (4 citations) Acetyl‐FoxO1 (2 citations)

5 protocols using foxo1

1

Transcriptional Profiling of HUVECs

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HUVECs were transfected with APLN, APLNR, FOXO1, or negative control siRNAs (Invitrogen). After 48 hours, RNA was extracted using miRNeasy Mini kit (Qiagen). The RNA was quantified, and the RNA quality was verified using NanoDrop (Thermo Fisher Scientific). The HumanHT-12 v4 Expression BeadChip kit (Illumina) was used according to the manufacturer’s protocol by the Yale Center for Genome Analysis. Microarray results were analyzed using the bead array and limma packages in R/Bioconductor (version 2.14/2.09). The gene expression profiling data have been deposited into GEO (GSE67390).
Hwangbo C., Wu J., Papangeli I., Adachi T., Sharma B., Park S., Zhao L., Ju H., Go G.W., Cui G., Inayathullah M., Job J.K., Rajadas J., Kwei S.L., Li M.O., Morrison A.R., Quertermous T., Mani A., Red-Horse K, & Chun H.J. (2017). Endothelial APLNR regulates tissue fatty acid uptake and is essential for apelin’s glucose-lowering effects. Science translational medicine, 9(407), eaad4000.
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2

Histological Analysis of Tumor Samples

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Tumors were fixed in formalin overnight before paraffin embedding. Paraffin blocks were sectioned and stained with H&E at the Dana-Farber/Harvard Cancer Center Rodent Histopathology Core. Immunohistochemistry was carried out using the antibodies Ki67 (Vector), FoxO1, p-Akt (Ser473) (Invitrogen), p-S6rp and p-Erk (Cell Signaling Technology). TUNEL assays were conducted using the ApoTag Plus Peroxidase in situ TUNEL Apoptosis Kit (Millipore) according to the manufacturer’s instructions.
Cheng H., Liu P., Ohlson C., Xu E., Symonds L., Isabella A., Muller W.J., Lin N.U., Krop I.E., Roberts T.M., Winer E.P., Arteaga C.L, & Zhao J.J. (2015). PIK3CAH1047R and Her2 initiated mammary tumors escape PI3K dependency by compensatory activation of MEK-ERK signaling. Oncogene, 35(23), 2961-2970.
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3

Histological Analysis of Tumor Samples

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Tumors were fixed in formalin overnight before paraffin embedding. Paraffin blocks were sectioned and stained with H&E at the Dana-Farber/Harvard Cancer Center Rodent Histopathology Core. Immunohistochemistry was carried out using the antibodies Ki67 (Vector), FoxO1, p-Akt (Ser473) (Invitrogen), p-S6rp and p-Erk (Cell Signaling Technology). TUNEL assays were conducted using the ApoTag Plus Peroxidase in situ TUNEL Apoptosis Kit (Millipore) according to the manufacturer’s instructions.
Cheng H., Liu P., Ohlson C., Xu E., Symonds L., Isabella A., Muller W.J., Lin N.U., Krop I.E., Roberts T.M., Winer E.P., Arteaga C.L, & Zhao J.J. (2015). PIK3CAH1047R and Her2 initiated mammary tumors escape PI3K dependency by compensatory activation of MEK-ERK signaling. Oncogene, 35(23), 2961-2970.
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4

Molecular Analysis of MIN6 Cell Regulation

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MIN6 cell culture, RNA isolation and first-strand cDNA synthesis, and preparation of pLKO.1-Pdx1 short hairpin RNA (shRNA) lentivirus all were performed as previously described (16 (link)). TaqMan assay numbers (Invitrogen) were as follows: mouse actin B, 4352933; IRS2, Mm003038438_m1; Bim, Mm00437796_m1; and Puma, Mm00519268_m1. The pLKO-Bim shRNA (TRCN0000009692), IRS2 shRNA (TRCN00000055110), and FoxO1 (TRCN0000054880) lentiviral vectors were purchased from Thermo Scientific. Lentivirus was added to the medium on day 1. The blots were probed with antibodies against IRS2 (3089; Cell Signaling), Puma (7467; Cell Signaling), cleaved caspase-3 (9661; Cell Signaling), FoxO1 (2880; Cell Signaling), p-AKT and AKT (9916; Cell Signaling), Bcl-xL (2762; Cell Signaling), Bcl-2 (554218; Pharmingen), BAD (sc-943; Santa Cruz Biotechnology), Mcl-1 (sc-819; Santa Cruz Biotechnology), Bim (202000; Calbiochem), and β-actin (A-2066; Sigma-Aldrich).
Ren D., Sun J., Mao L., Ye H, & Polonsky K.S. (2014). BH3-Only Molecule Bim Mediates β-Cell Death in IRS2 Deficiency. Diabetes, 63(10), 3378-3387.
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5

Quantitative Real-Time PCR Protocol

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The PCR reactions were performed based on Taq-Man chemistry using the probes specific for the following genes: Csf1 (Mm00432686_m1), Ccr6 (Mm99999114_s1), Cxcr4 (Mm01996749_s1), Drg1 (Mm00492246_m1), Foxo1 (Mm00490672_m1), Nf2 (Mm00477771_m1), Nedd9 (Mm01324843_m1), Mmp14 (Mm00485054_m1), Spp1 (Mm00436767_m1), Flt1 (Mm00438980_m1), Plaur (Mm01149438_m1), Tgfb1 (Mm01178820_m1), Pgk1 (Mm00435617_m1), and Rpl13a (Mm01612987_g1) (all Thermo Fisher Scientific, Waltham, MA, USA). Each amplification cycle was performed at 95 °C for 15 s and at 60 °C for 1 min (total 40 cycles) in ViiA™ 7 Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA). Twenty-five nanograms of cDNA were used for a single reaction and each sample was prepared in triplets (technical repetition). The relative quantification level of examined gene expression, referred to as fold change, was calculated based on differences in ΔΔCt values of the studied genes in relation to control housekeeping genes (Pgk1 or Rpl13a) by using DataAssist 3.01 software (Thermo Fisher Scientific, Waltham, MA, USA).
Anisiewicz A., Pawlik A., Filip-Psurska B, & Wietrzyk J. (2020). Differential Impact of Calcitriol and Its Analogs on Tumor Stroma in Young and Aged Ovariectomized Mice Bearing 4T1 Mammary Gland Cancer. International Journal of Molecular Sciences, 21(17), 6359.
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