Three to six capsules were harvested from controls and each treatment, air-dried, opened on stubs to disperse the spores, and processed according to Rabbi et al. (2020) (link). Germinated spores were fixed per the TEM protocol and critical point dried rather than being infiltrated with resin. The specimens were sputter-coated with 50 nm of Au/Pd using a Denton Desk II Vacuum Sputter Coater and imaged using a scanning electron microscope (SEM) (QUANTA FEG 450; FEI) with 5 kV acceleration voltage.
Desk 2 vacuum sputter coater
Manufactured by Thermo Fisher Scientific
The Desk II Vacuum Sputter Coater is a laboratory equipment designed for the deposition of thin conductive films onto samples. It operates under vacuum conditions and utilizes a sputtering process to deposit the desired material onto the sample surface.
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Lab products found in correlation
2 protocols using desk 2 vacuum sputter coater
1
SEM Imaging of Fungal Spore Germination
2
Spore Dispersion SEM Imaging
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