Annexin 5 fitc pi staining kit
The Annexin V-FITC/PI staining kit is a reagent used for the detection of apoptosis in cell samples. It utilizes Annexin V, a calcium-dependent phospholipid-binding protein, and propidium iodide (PI) to identify cells in different stages of apoptosis and necrosis.
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15 protocols using annexin 5 fitc pi staining kit
Apoptosis Evaluation via Annexin V-FITC/PI Staining
Cell Cycle and Apoptosis Analysis
Apoptosis Analysis by Flow Cytometry
DNA fragmentation and apoptosis analysis
The effect of AB2 on cell apoptosis was evaluated by flow cytometer with Annexin V-FITC/PI staining kit (Thermo Fisher Scientific, CA, USA), according to the manufacturer’s instructions. Briefly, the cells were treated with AB2 compound at IC50 and 2 × IC50 concentrations for 24 h. After harvesting, cells were suspended in 300 μL binding buffer, and then stained with Annexin-FITC and/or propium iodide. Positive controls for apoptosis were stained with only Annexin-FITC. Positive controls for necrosis were stained with only propium iodide. At least 104 cells were analyzed by flow cytometer (BD, Biosciences, San Jose, CA, USA) and data were analyzed using FlowJo vX.0.7 (Tree Star, Inc., Ashland, OR, USA).
Parthenolide Modulates EGFR-ERK-AKT Signaling
Quantifying Cell Apoptosis by Flow Cytometry
Flow Cytometric Analysis of Apoptosis
Examining Apoptosis Signaling Pathways
Apoptosis Measurement: TUNEL and Flow
Apoptosis Evaluation of HCT15 and SW116 Cells
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