Cells were fixed and permeabilized (20 min with Cytofix/Cytoperm; BD Biosciences), washed (Cytoperm Buffer; BD Biosciences), and then blocked with casein protein (DAKO; Carpinteria, CA) for 1 h. For detection of NF-κB p65, cells were incubated with rabbit anti-NF-κB p65 and mouse anti- HCMV p63-27, specific for IE1, or irrelevant antibody (0.05 mg/mL) for 90 min (Santa Cruz Biotechnology, Santa Cruz, CA). Cells were washed and then incubated with donkey anti-rabbit IgG-FITC or anti-mouse IgG-PE (30 min, 1:500; Jackson ImmunoResearch Laboratories, West Grove, PA). For detection of Smad7, cells were incubated with rabbit anti-Smad7 or irrelevant antibody (overnight at 4°C, 1:50; Santa Cruz Biotechnology, Dallas, TX), washed, and then incubated with donkey anti-rabbit IgG-Cy (45 min, 1:500; Jackson ImmunoResearch Laboratories, West Grove, PA). Cells were washed with PBS and counter-stained with DAPI, and then visualized by confocal microscopy.
Rabbit anti smad7 or irrelevant antibody
Manufactured by Santa Cruz Biotechnology
Rabbit anti-Smad7 is a polyclonal antibody raised in rabbits against the Smad7 protein. It is designed for use in various immunological techniques, such as western blotting and immunohistochemistry, to detect and study the Smad7 protein.
Automatically generated - may contain errors
Lab products found in correlation
Fitc donkey anti rabbit igg, by Jackson ImmunoResearch (2 mentions)
Cytoperm buffer, by BD (2 mentions)
Casein protein, by Agilent Technologies (2 mentions)
Donkey anti rabbit igg cy, by Jackson ImmunoResearch (2 mentions)
Anti mouse igg pe, by Jackson ImmunoResearch (2 mentions)
Cytofix cytoperm, by BD (2 mentions)
2 protocols using rabbit anti smad7 or irrelevant antibody
1
Immunofluorescence Staining of NF-κB and Smad7
2
Immunofluorescence Staining of NF-κB and Smad7
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were fixed and permeabilized (20 min with Cytofix/Cytoperm; BD Biosciences), washed (Cytoperm Buffer; BD Biosciences), and then blocked with casein protein (DAKO; Carpinteria, CA) for 1 h. For detection of NF-κB p65, cells were incubated with rabbit anti-NF-κB p65 and mouse anti- HCMV p63-27, specific for IE1, or irrelevant antibody (0.05 mg/mL) for 90 min (Santa Cruz Biotechnology, Santa Cruz, CA). Cells were washed and then incubated with donkey anti-rabbit IgG-FITC or anti-mouse IgG-PE (30 min, 1:500; Jackson ImmunoResearch Laboratories, West Grove, PA). For detection of Smad7, cells were incubated with rabbit anti-Smad7 or irrelevant antibody (overnight at 4°C, 1:50; Santa Cruz Biotechnology, Dallas, TX), washed, and then incubated with donkey anti-rabbit IgG-Cy (45 min, 1:500; Jackson ImmunoResearch Laboratories, West Grove, PA). Cells were washed with PBS and counter-stained with DAPI, and then visualized by confocal microscopy.
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