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Cd31 pe cy7 clone 390

Manufactured by Thermo Fisher Scientific

CD31 PE-Cy7 (clone 390) is a fluorescently-labeled antibody that binds to the CD31 cell surface antigen. CD31 is a cell adhesion molecule expressed on the surface of endothelial cells, platelets, and certain immune cells. This antibody can be used to detect and analyze cells expressing CD31 in various research applications.

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2 protocols using cd31 pe cy7 clone 390

1

Runx1-GFP Mouse Embryo Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Timed matings of Runx1GFP mice (Runx1tm4Dow)43 (link) were performed to generate embryos, with vaginal plug considered as embryonic day (E) 0.5. Embryos with 32–40 somite pairs were staged as E10.5. AGM and YS were disaggregated with collagenase, as previously described44 (link). The following mouse Abs were used: CD31 PE-Cy7 (clone 390, eBioscience, 1:200), CD41-PerCP-eFluor710 (clone eBioMWReg30, eBioscience, 1:200), CD45-PerCP-Cy5.5 (clone 102, BD Pharmingen, 1:200), Ter119-eFlour450 (clone TER-119, eBioscience, 1:200), CD184-APC (clone 2B11, BD Pharmingen, 1:200), CD73-PE (clone eBioTY/11.8, eBioscience, 1:200). DAPI (Life Technologies) was used to exclude dead cells. No statistical method was used to predetermine sample size. The experiments were not randomized. The investigators were not blinded to allocation during experiments and outcome assessment.
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2

Runx1-GFP Mouse Embryo Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Timed matings of Runx1GFP mice (Runx1tm4Dow)43 (link) were performed to generate embryos, with vaginal plug considered as embryonic day (E) 0.5. Embryos with 32–40 somite pairs were staged as E10.5. AGM and YS were disaggregated with collagenase, as previously described44 (link). The following mouse Abs were used: CD31 PE-Cy7 (clone 390, eBioscience, 1:200), CD41-PerCP-eFluor710 (clone eBioMWReg30, eBioscience, 1:200), CD45-PerCP-Cy5.5 (clone 102, BD Pharmingen, 1:200), Ter119-eFlour450 (clone TER-119, eBioscience, 1:200), CD184-APC (clone 2B11, BD Pharmingen, 1:200), CD73-PE (clone eBioTY/11.8, eBioscience, 1:200). DAPI (Life Technologies) was used to exclude dead cells. No statistical method was used to predetermine sample size. The experiments were not randomized. The investigators were not blinded to allocation during experiments and outcome assessment.
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