Phosphor fak tyr397

Recombinant Human Epidermal Growth Factor (EGF) and Hepatocyte Growth Factor (HGF) were purchased from Peprotech. SGX523 was purchased from GE and Erlotinib from Cayman chemicals; both were solubilised in DMSO. All antibodies (Abs) were purchased from commercial sources as indicated. For tissue and cell staining, Phospho-Histone H3 was from Millipore, α-SMA from AnaSpec Inc., cd31 from Abcam, total cMET (D1C2) from Cell Signaling Technology and EGFR Ab from Novocastra/Leica. For immunoprecipitation, the EGFR Ab was from Santa Cruz Biotechnology (sc-120). For Western Blot, phospho-EGFR (Y1173), total EGFR phospho-MET (Y1234), total MET, phospho-ERK (Thr202/Tyr204), total ERK, phopho-AKT (Ser473), total AKT, phosphor-FAK (Tyr397) and total FAK were from Cell Signaling Technology; tubulin was from Millipore and hsc70 from Santa Cruz Biotechnology. For proliferation assays, anti-Brdu was purchased from Abcam.

SAHA (#S1047), LBH589 (#S1089), DefActinib (#S7654) and Tasquinimod (#7617) were received from Selleck (Texas, USA). Antibodies NEDD9 (#4044), phosphor-Src-Tyr416 (#6943), Src (#2108 S), phosphor-FAK-Tyr397 (#8556 S), FAK (#3285 S), HDAC1 (#34589 T), HDAC2 (#5113 T), HDAC3(#3949 S), HDAC4 (#5392 S), HDAC5 (#20458 S), HDAC6 (#7558 S) were purchased from Cell Signaling Technology (Massachusetts, USA). Ac-H3K9 (#ab10812) was purchased from Abcam (Cambridge, UK). vinculin(#66305-1-Ig), Histone H3 (17168-1-AP), Actin (#60008-1-Ig), were purchased from Proteintech (Chicogo, USA).

Acetyl-CoA, Malonyl-CoA, NADPH, DMSO, and α-mangostin were purchased from Sigma (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Life Technologies, Inc. (Gibco/BRL, Gaithersburg, MD). FAS antibody was obtained from BD Pharmingen (San Diego, CA, USA). FAK, phosphor-FAK^tyr397, AKT, phospho-AKT^Ser473, ERK1/2, phosphor-ERK1/2^{Thr202/Tyr204}, Bax, Bcl-2, PARP and GAPDH were purchased from Cell Signaling Technology (Denvers, MA, USA).