Hrp labeled anti mouse and anti rabbit secondary antibody

Manufactured by Santa Cruz Biotechnology

HRP-labeled anti-mouse and anti-rabbit secondary antibodies are laboratory reagents used for the detection of primary antibodies in various immunoassay techniques, such as Western blotting and ELISA. These secondary antibodies are conjugated with the enzyme horseradish peroxidase (HRP), which enables the amplification and visualization of the target signal.

Automatically generated - may contain errors

Lab products found in correlation

Complete protease cocktail, by Roche (2 mentions) Antibody to raptor, by Merck Group (2 mentions) Antibody to the ha epitope, by Fortis Life Sciences (2 mentions) Xdp and xtp, by Jena Biosciences (2 mentions) 3h labeled gtp and gdp, by PerkinElmer (2 mentions) Inactivated fetal calf serum ifs and simply blue stain, by Thermo Fisher Scientific (2 mentions) Alexa fluor 594 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Pp2ac, by Abcam (1 mentions) P tsc2 t1462, by Cell Signaling Technology (1 mentions) Insulin, by Thermo Fisher Scientific (1 mentions) Protein a and protein g agarose beads, by Thermo Fisher Scientific (1 mentions) Anti ha, by Roche (1 mentions) Protease and phosphatase inhibitor cocktail, by Roche (1 mentions) Anti ha h6908, by Merck Group (1 mentions) Egfr sc 03, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Abcam (1 mentions) Docetaxel, by Cell Signaling Technology (1 mentions) Perifosine krx 0401, by Selleck Chemicals (1 mentions)

7 protocols using hrp labeled anti mouse and anti rabbit secondary antibody

Antibody-based Signaling Pathway Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies are purchased from the following vendors: P-TSC2(S1798) (sc-293149), Santa-Cruz Biotechnology; P-TSC2(S664) (ab133465) and S6K1 (ab32529), Abcam; PP2AA (#2041), PP2AB (#2290), PP2AC (#2059), P-S6K1(T398) (#9234), P-AKT(S473) (#4060), AKT (#9272), P-MK2(T334) (#3007), MK2 (#3042), P-Hsp27 (S82) (#9709), Hsp27 (#2402), P-S6(S235/236) (#4858), S6 (#2217), P-ERK(T202/Y204) (#4370), ERK (#4695), P-RSK(S380) (#9335), RSK (#9355), P-TSC2 (S1254) (#3616), P-TSC2(T1462) (#3617), TSC2 (#4308), mTOR (#2983), PARP (#9532), Ki67 (#9449), and Tubulin (#3873), Cell Signaling Technology; HRP-labeled anti-mouse and anti-rabbit secondary antibodies, Santa-Cruz Biotechnology and Alexa Fluor 594 conjugated secondary antibody, Invitrogen. Western blot analysis was performed as described previously (Thomas et al., 2014 (link)). For immunoprecipitation, CRC cells were treated with SB202190 10 μM for 2 h prior to lysis. Then cells were lysed with NP40 lysis buffer (50 mM Tris–HCl at pH 8.0, 150 mM NaCl, 5 mM EDTA, 0.5% NP40), and diluted lysates were applied to immunoprecipitation with anti-PP2AC antibody and immunoblotting with anti- ERK and anti-PP2AC antibody.

Zhang Y., Wang X., Qin X., Wang X., Liu F., White E, & Zheng X.F. (2015). PP2AC Level Determines Differential Programming of p38-TSC-mTOR Signaling and Therapeutic Response to p38-Targeted Therapy in Colorectal Cancer. EBioMedicine, 2(12), 1944-1956.

+ Open protocol

+ Expand

Antibodies and Reagents Sources

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies were obtained from the following sources. Antibodies against IKKα, IKKβ, and mTOR were obtained from Upstate Biotechnology. Raptor and Rictor antibodies were obtained from Bethyl Laboratories. Anti-HA and anti-Flag antibodies were obtained from Roche and Sigma, respectively. Anti-Actin was obtained from Calbiochem. The anti-S6K and control rabbit IgG, as well as HRP-labeled anti-mouse and anti-rabbit secondary antibodies were from Santa Cruz Biotechnology. Recombinant Human inactive Akt is from Upstate Biotechnology. All other antibodies were from Cell Signaling. Other reagents were obtained from the following sources: Insulin was from Invitrogen Corporation. Protease and phosphatase inhibitor cocktails were from Roche. The CHAPS was from Pierce. Protein A and protein G agarose beads were from Invitrogen Life Technologies.

Dan H.C., Antonia R.J, & Baldwin A.S. (2016). PI3K/Akt promotes feedforward mTORC2 activation through IKKα. Oncotarget, 7(16), 21064-21075.

+ Open protocol

+ Expand

Antibody Sources for Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies were obtained from the following sources: Antibodies against phospho-p65 (CST-3033), p65 (CST-6956), phospho-IKKα/β (CST-2697), IKKα (CST-2682), IKKβ (CST-8943), phospho-Akt (CST-4508), Akt1 (CST-2938), Akt2 (CST-3063), phospho-S6K (CST-9205), mTOR (CST-2972), cleaved caspase 3 (CST-9664), and GAPDH (CST-5174), are purchased from Cell Signaling. Anti-Raptor ((A300-506A) and anti-Rictor (A300-458A) are from Bethyl. Anti-HA (H6908) is from Sigma. Anti-S6K (SC-8418), C-Myc (SC-40), and EGFR (SC-03) are from Santa Cruz Biotechnology. HRP-labeled anti-mouse and anti-rabbit secondary antibodies were also from Santa Cruz Biotechnology.

Li Z., Yang Z., Passaniti A., Lapidus R.G., Liu X., Cullen K.J, & Dan H.C. (2016). A positive feedback loop involving EGFR/Akt/mTORC1 and IKK/NF-κB regulates head and neck squamous cell carcinoma proliferation. Oncotarget, 7(22), 31892-31906.

+ Open protocol

+ Expand

Signaling Pathway Antibody Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies against IKKα, IKKβ, mTOR, Akt1, and Akt2 were obtained from Upstate Biotechnology. Anti-Raptor and anti-Rictor antibodies were obtained from Bethyl Laboratories. The anti-myc (9E-10), anti-TSC2 and control rabbit IgG, as well as HRP-labeled anti-mouse and anti-rabbit secondary antibodies were from Santa Cruz Biotechnology. All other antibodies were from Cell Signaling Technology.

Gao Y., Gartenhaus R.B., Lapidus R.G., Hussain A., Zhang Y., Wang X, & Dan H.C. (2015). Differential IKK/NF-κB Activity is Mediated by TSC2 through mTORC1 in PTEN-null Prostate Cancer and Tuberous Sclerosis Complex Tumor Cells. Molecular cancer research : MCR, 13(12), 1602-1614.

+ Open protocol

+ Expand

Molecular Mechanisms of Perifosine and Docetaxel

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Akt inhibitor Perifosine (KRX-0401) was purchased from Selleck Chemical. Docetaxel was purchased from Cell Signaling. The IKKβ inhibitor, CmpdA, was kindly provided by Dr. Albert Baldwin, University of North Carolina. Antibodies against IKKα, IKKβ, and Akt were obtained from Upstate Biotechnology. Antibodies for immunohistochemistry (IHC), including Ki67, cleaved caspase-3, p-IKKα/β-S177/S181, Slug and Snail were from Abcam. HRP-labeled anti-mouse and anti-rabbit secondary antibodies were from Santa Cruz Biotechnology. Antibodies for Oct4 (CST-2750), Sox2 (CST-3728), p65 (CST-8242), p-p65 (CST-3033), Survivin (CST-2808), p-Akt-S473 (CST-4058), GAPDH (CST-5174), as well as any additional antibodies were from Cell Signaling.

Zhang Y., Lapidus R.G., Liu P., Choi E.Y., Adediran S., Hussain A., Wang X., Liu X, & Dan H.C. (2016). Targeting IκB kinase β/NF-κB signaling in human prostate cancer by a novel IκB kinase β inhibitor CmpdA. Molecular cancer therapeutics, 15(7), 1504-1514.

+ Open protocol

+ Expand

Antibody-Based Protein Complex Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagents were obtained from the following sources: HRP-labeled anti-mouse and anti-rabbit secondary antibody from Santa Cruz Biotechnology; antibodies to phospho-T389 S6K1, S6K1, mTOR, and FLAG epitope from Cell Signaling Technology; antibody to the HA epitope from Bethyl laboratories; antibody to raptor from Millipore. RPMI, FLAG M2 affinity gel, GTP, GDP, and amino acids from Sigma Aldrich; XDP and XTP from Jena Biosciences; [³H]-labeled GTP and GDP from Perkin Elmer; DMEM from SAFC Biosciences; Complete Protease Cocktail from Roche; Inactivated Fetal Calf Serum (IFS) and simply blue stain from Invitrogen; amino acid-free RPMI from US Biologicals.

Okosun J., Wolfson R.L., Wang J., Araf S., Wilkins L., Castellano B.M., Escudero-Ibarz L., Al Seraihi A.F., Richter J., Bernhart S.H., Efeyan A., Iqbal S., Matthews J., Clear A., Guerra-Assunção J.A., Bödör C., Quentmeier H., Mansbridge C., Johnson P., Davies A., Strefford J.C., Packham G., Barrans S., Jack A., Du M.Q., Calaminici M., Lister T.A., Auer R., Montoto S., Gribben J.G., Siebert R., Chelala C., Zoncu R., Sabatini D.M, & Fitzgibbon J. (2015). Recurrent mTORC1-activating RRAGC mutations in follicular lymphoma. Nature genetics, 48(2), 183-188.

+ Open protocol

+ Expand

Antibody-Based Protein Complex Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!