Ab126786

For IHC staining, the TMAs containing 118 tumor sections and 118 adjacent normal tissue sections were obtained from Zhongshan Hospital, Fudan University (Shanghai, China). Immunohistochemistry (IHC) staining was performed as previously described
[25] (link), using marker antibodies anti-human C7 (ab126786; Abcam, Cambridge, UK), and anti-human C9 (ab168345; Abcam), and scanned using Pannoramic MIDI (3DHISTECH Ltd. Budapest, Hungary). In order to quantify the staining intensity, the scanned image was analyzed with the image analysis system based on artificial intelligence, which calculated the modified Histochemistry-scores as follows: H-scores=(percent of weak staining×1)+(percent of moderate staining×2)+(percent of strong staining×3)
[26] (link). Finally, the H-scores ranging from 0 to 300 were obtained.

Four-micrometre tissue sections from formalin-fixed, paraffin-wax-embedded human endometriosis tissues and endometrial tissues were dewaxed, rehydrated, and subjected to high-temperature antigen retrieval. The tissues were incubated with blocking antibody diluent at room temperature for 2 h, and then incubated overnight at 4 °C with the following primary antibodies: anti-vimentin (mouse, ab8978, dilution 1:1000; Abcam), anti-CXCL8 (rabbit, sc-8427, dilution 1:150; Santa Cruz), anti-C7 (rabbit, ab126786, dilution 1:150; Abcam), anti-S100A10 (rabbit, sc-81153, dilution 1:150; Santa Cruz), anti-C3 (rabbit, ab200999, dilution 1:150; Abcam), and anti-StAR (rabbit, sc-166821, dilution 1:150; Santa Cruz). The slides were then incubated with secondary antibody (HRP polymer, anti-mouse/rabbit IgG, Abcam) at room temperature for 1 h. Nuclei were stained with 4-6-diamidino-2-phenylindole (DAPI, ab104139, Abcam) after all antigens had been labelled and then imaged.