Envision 2104 plate reader

Manufactured by PerkinElmer

Sourced in United States

The Envision 2104 plate reader is a high-performance multi-mode microplate reader capable of measuring various types of assays. It offers a range of detection modes, including absorbance, fluorescence, and luminescence, enabling researchers to analyze a diverse set of samples and applications.

Automatically generated - may contain errors

Lab products found in correlation

Janus workstation pin tool, by PerkinElmer (6 mentions) 384 well white culture plates, by Thermo Fisher Scientific (4 mentions) Prism v6, by GraphPad (4 mentions) 384 well white culture plates, by Corning (2 mentions) Passive lysis buffer (plb), by Promega (2 mentions) Atplite kit, by PerkinElmer (2 mentions) Alphaplate, by PerkinElmer (2 mentions) Renilla glo luciferase assay system, by Promega (1 mentions) Envision 2104, by PerkinElmer (1 mentions) Celltiter glo, by Promega (1 mentions) Fluostar omega reader, by BMG Labtech (1 mentions) Atplite 1step luminescence assay system, by PerkinElmer (1 mentions) Dual glo stop glo reagent, by Promega (1 mentions) Dual glo reagent, by Promega (1 mentions) Proxiplate, by PerkinElmer (1 mentions) Renilla luciferase, by Promega (1 mentions) Supertopflash, by Promega (1 mentions) Alphascreen plate, by PerkinElmer (1 mentions) Janus workstation, by PerkinElmer (1 mentions) Celltiter glo luminescent cell viability assay kit, by Promega (1 mentions)

20 protocols using envision 2104 plate reader

In Vitro Human Translation Assay

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The reconstitution system for human translation has been described previously (Iwasaki et al., 2019 (link); Machida et al., 2018 (link); Yokoyama et al., 2019 (link)). The in vitro translation reaction and luciferase assay were performed as previously described (Iwasaki et al., 2019 (link)) with some modifications. The final concentrations of mRNA and the eIF4A protein were 60 ng/µl and 2.16 µM, respectively. The translation mixture was incubated for 2.5 hr. The fluorescence signal was detected using the Renilla-Glo Luciferase Assay System (Promega) and measured in an EnVision 2104 plate reader (PerkinElmer).

Chen M., Kumakura N., Saito H., Muller R., Nishimoto M., Mito M., Gan P., Ingolia N.T., Shirasu K., Ito T., Shichino Y, & Iwasaki S. (2023). A parasitic fungus employs mutated eIF4A to survive on rocaglate-synthesizing Aglaia plants. eLife, 12, e81302.

+ Open protocol

+ Expand

Compound Screening in MV4-11 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

MV4;11 cells expressing luciferase-FKBP12^F36V were plated at 2000 cells per well in 50 µL of appropriate media in 384-well white culture plates (Thermo Fisher Scientific). 100 nL of compound in DMSO from compound stock plates was added to each well using a Janus Workstation pin tool (PerkinElmer). After addition of compound, plates were incubated for 16 hours at 37 ºC, prior to luminescence assessment. Plates were brought to room temperature prior to reagent addition. 25 µL Fluc buffer (as described above for the FKBP12^WT and FKBP12^F36V dual luciferase assay) was added to each well for 10 minutes at room temperature, and luminescence was measured on the Envision 2104 plate reader (PerkinElmer). Luminescence values were then normalized by setting DMSO only wells to 100%. Data were analyzed and plotted using GraphPad PRISM v6 using the ‘log(inhibitor) vs. response -- Variable slope (four parameters)’ analysis module.

Nabet B., Roberts J.M., Buckley D.L., Paulk J., Dastjerdi S., Yang A., Leggett A.L., Erb M.A., Lawlor M.A., Souza A., Scott T.G., Vittori S., Perry J.A., Qi J., Winter G.E., Wong K.K., Gray N.S, & Bradner J.E. (2018). The dTAG system for immediate and target-specific protein degradation. Nature chemical biology, 14(5), 431-441.

+ Open protocol

+ Expand

Quantitative Cell Viability Assay

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell viability was assayed in 2D-adherent or ultra-low adherent 3D-spheroids using CellTiter-Glo (Promega)^{6 (link),15 (link)}. Luminescence was measured on an Envision 2104 plate reader (PerkinElmer) and Fluostar Omega Reader (BMG Labtech) and data was analyzed using GraphPad PRISM v8. Synergy assessments were performed using CellTiter-Glo (Promega) with the following modifications to the protocol described in the ref. ^{34 (link)}. In brief, EWS502 cells were plated at 1000 cells per well in 50 µL of appropriate media in 384-well white culture plates (Corning) allowed to adhere overnight, and 100 nL of compounds were added using a Janus Workstation pin tool (PerkinElmer) for 72 h. Cell viability was measured by addition of 10 µL of CellTiter-Glo (Promega), followed by incubation for 15 minutes at room temperature. Luminescence was measured on an Envision 2104 plate reader (PerkinElmer) and data was analyzed using GraphPad PRISM v8.

Nabet B., Ferguson F.M., Seong B.K., Kuljanin M., Leggett A.L., Mohardt M.L., Robichaud A., Conway A.S., Buckley D.L., Mancias J.D., Bradner J.E., Stegmaier K, & Gray N.S. (2020). Rapid and direct control of target protein levels with VHL-recruiting dTAG molecules. Nature Communications, 11, 4687.

+ Open protocol

+ Expand

Quantifying Cellular Luciferase Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols

For luciferase assays, cells were lysed, after washing twice with 1xPBS, by adding passive lysis buffer (Promega) directly onto the plates following the manufacturer’s protocol. Extracts were collected and centrifuged at 14000 rpm for 5 min. 50 µl of cleared extract was transferred into each well of a 96 well plate (#655083, Greiner Bio-one) containing 50 µl of 2 x Luciferase Buffer (50 mM Tris/phosphate (pH 7.8), 16 mM MgCl2, 2 mM DTT (dithiothreitol), 30% (v/v) glycerol, 1 mM ATP, 1% BSA, 0.25 mM luciferin and 8 μM sodium pyrophosphate) and the data was obtained using an Envision 2104 plate reader (PerkinElmer). Protein concentration, determined by BCA Protein Assay Reagent (Pierce), was used for data normalisation.

Rojas-Fernandez A., Herhaus L., Macartney T., Lachaud C., Hay R.T, & Sapkota G.P. (2015). Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9. Scientific Reports, 5, 9811.

+ Open protocol

+ Expand

Cell Viability Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were plated at 1000 cells/well in 50 µL/well of media in 384 well white culture plates (Thermo). NMC797 cells were allowed to adhere overnight before adding 100 nL of compound in DMSO from compound stock plates using a Janus Workstation pin tool (PerkinElmer). After addition of compound, plates were incubated for 72 hours at 37°C. Cell viability was read out using the ATPlite kit (PerkinElmer). Plates were brought to room temperature prior to reagent addition. Lyophilized powder was resuspended in lysis buffer and diluted 1:2 with DI water. 20 µL of this solution was added to each well and plates were incubated for 15 min at room temperature before signal was read on an Envision 2104 plate reader (Perkin Elmer).

Tanaka M., Roberts J.M., Seo H.S., Souza A., Paulk J., Scott T.G., DeAngelo S.L., Dhe-Paganon S, & Bradner J.E. (2016). Design and Characterization of Bivalent BET Inhibitors. Nature chemical biology, 12(12), 1089-1096.

+ Open protocol

+ Expand

High-Throughput Cellular Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

293T and NIH/3T3 cells were plated at 1000 cells per well in 50 µL of appropriate media in 384-well white culture plates (Thermo Fisher Scientific). Cells were allowed to adhere overnight before adding 100 nL of compound in DMSO from compound stock plates using a Janus Workstation pin tool (PerkinElmer). After addition of compound, plates were incubated for 72 hours at 37 ºC, and ATPlite 1 Step Luminescence Assay System (PerkinElmer) was used to determine ATP-dependent luminescence as an approximation of cellular viability. Plates were brought to room temperature prior to reagent addition. Lyophilized powder was resuspended in lysis buffer and diluted 1:3 with deionized water. 20 µL of this solution was added to each well, plates were incubated for 15 minutes at room temperature, and luminescence was measured on an Envision 2104 plate reader (PerkinElmer). Cell viability values were calculated by normalizing to DMSO treated wells for each cell line. Data were analyzed and plotted using GraphPad PRISM v6 and the ‘log(inhibitor) vs. response -- Variable slope (four parameters)’ analysis module.

+ Open protocol

+ Expand

FKBP12 Binding Inhibition Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

In 384-well AlphaPlates (PerkinElmer), 90 nM GST-FKBP12 (WT or F36V) and 62.5 nM bio-SLF were diluted in 20 µL assay buffer (PBS containing 0.1% BSA and 0.01%Triton X-100) containing competitor compound or DMSO. Following a 30-minute incubation, 20 µL detection solution containing Streptavidin Donor Beads and Glutathione Acceptor Beads diluted to 20 ng/µL in assay buffer was added to each well. After a one hour incubation at room temperature, luminescence was measured on an Envision 2104 plate reader (PerkinElmer). Percent activity values were calculated by setting the average background (no protein wells) to 0% and the average DMSO wells to 100% activity. Data were analyzed and plotted using GraphPad PRISM v6 and IC₅₀ values were determined using the ‘log(inhibitor) vs. response -- Variable slope (four parameters)’ analysis module.

+ Open protocol

+ Expand

Dual Luciferase Assay for FKBP12 Variants

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dual luciferase assays were performed using 293FT FKBP12^WT-Nluc and FKBP12^F36V-Nluc cells^{6 (link)}. In brief, cells were plated at 2000 cells per well in 20 µL of appropriate media in 384-well white culture plates (Corning), allowed to adhere overnight, and 100 nL of compounds were added using a Janus Workstation pin tool (PerkinElmer) for 24 h at 37 °C. To evaluate Fluc signal, plates were brought to room temperature, 20 µL of Dual-Glo Reagent (Promega) was added for 10 min and luminescence was measured on an Envision 2104 plate reader (PerkinElmer). Subsequently, 20 µL of Dual-Glo Stop & Glo Reagent (Promega) was added for 10 min and luminescence was again measured to capture Nluc signal. DMSO-normalized ratios of Nluc/Fluc signal was analyzed and plotted using GraphPad PRISM v8^{6 (link)}.

+ Open protocol

+ Expand

Quantifying SMAD3, p38, ERK, JNK Phosphorylation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The phosphorylation state of SMAD3, p38, ERK1/2, and JNK1/2/3 was quantified using Amplified Luminescence Proximity Homogenous Assays (Perkin Elmer Waltham, Massachusetts, USA). A total of 12,000–15,000 cells/well were seeded in a 24-well plate. Forty-eight hours after plating, the cells were starved overnight in total absence of fetal bovine serum. They then were stimulated with different concentrations of TGF-β1 (0–100 ng/mL) for 1 hour, washed with phosphate-buffered saline, and lysed for 20 minutes at room temperature using 200 μL/well of AlphaScreen SureFire Ultra lysis buffer supplemented with protease inhibitors. The quantifications were performed in 384-well plates (Proxiplate; Perkin Elmer), according to protocol guidelines and measured with the EnVision 2104 plate reader (Perkin Elmer). For each condition, Six independent measurements were performed for each cell line and experiments were repeated four times.

Piché J., Gosset N., Legault L.M., Pacis A., Oneglia A., Caron M., Chetaille P., Barreiro L., Liu D., Qi X., Nattel S., Leclerc S., Breton-Larrivée M., McGraw S, & Andelfinger G. (2018). Molecular Signature of CAID Syndrome: Noncanonical Roles of SGO1 in Regulation of TGF-β Signaling and Epigenomics. Cellular and Molecular Gastroenterology and Hepatology, 7(2), 411-431.

+ Open protocol

+ Expand

Wnt Signaling Pathway Luciferase Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293 cells (4 × 10
⁴/cm
²) were seeded in 12-well plates. 24 h later, 200 ng of SuperTOPFlash, 20 ng of Renilla luciferase (Promega, E2261), and 100 ng of GFP or PAWS1-GFP plasmids were co-transfected as described above. After 24 h, cells were treated with L-Wnt3A or L-conditioned medium for 6 h. Cells were washed with PBS and lysed in Passive Lysis Buffer (Promega, E194A). Firefly and Renilla luciferase activities were measured as described previously
^{17 (link)}. Briefly, extracts were mixed 1:1 with 2x Luciferase Buffer (50 mM Tris/phosphate (pH 7.8), 16 mM MgCl
₂, 2 mM DTT (dithiothreitol), 30% (v/v) glycerol, 1 mM ATP, 1% BSA, 0.25 mM luciferin and 8 μM sodium pyrophosphate) and light emission was measured using a Envision 2104 plate reader (PerkinElmer). An equivalent volume of 3x Renilla Assay Buffer (45 mM Na
₂EDTA, 30 mM sodium pyrophosphate, 1.425 M NaCl, 60 µM PTC124, 10 µM h-Coelenterazine) was then added, and Renilla luciferase emission was measured. The firefly luciferase counts were normalised to Renilla for each sample.

Wu K.Z., Jones R.A., Tachie-Menson T., Macartney T.J., Wood N.T., Varghese J., Gourlay R., Soares R.F., Smith J.C, & Sapkota G.P. (2020). Pathogenic FAM83G palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.. Wellcome Open Research, 4, 133.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!