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Chip kit

Manufactured by Merck Group
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The ChIP kit is a laboratory tool used for chromatin immunoprecipitation (ChIP) experiments. It provides a method to study protein-DNA interactions within the cellular environment.

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Lab products found in correlation

Ab13537, by Abcam (9 mentions) Formaldehyde, by Merck Group (6 mentions) Ab2851, by Abcam (5 mentions) Ab8898, by Abcam (4 mentions) Sonic dismembrator model 500, by Thermo Fisher Scientific (4 mentions) Ab2850, by Abcam (4 mentions) Annexin 5 kit, by Merck Group (3 mentions) Rna polymerase 2, by Santa Cruz Biotechnology (3 mentions) Anti kmt2a antibody, by Abcam (3 mentions) Anti h3k4me3 antibody, by Abcam (3 mentions) Magna chirp rna interactome kit, by Merck Group (3 mentions) Anti brg1, by Abcam (3 mentions) Ab6721, by Abcam (3 mentions) Lightcycler 480, by Roche (3 mentions) H3k4me3, by Merck Group (3 mentions) Sodium butyrate, by Merck Group (2 mentions) H3k4ac, by Santa Cruz Biotechnology (2 mentions) Hdac1, by Santa Cruz Biotechnology (2 mentions) Limulus amebocyte lysate qcl 1000, by Lonza (2 mentions) Immune cell isolation kits, by Miltenyi Biotec (2 mentions)

Close spelling variants of this product

EZ-ChIP kit (596 citations) EZ ChIP™ Chromatin Immunoprecipitation Kit (240 citations) EZ-Magna ChIP kit (151 citations) EZ-Magna ChIP A/G kit (87 citations) Magna ChIP A/G kit (69 citations) Magna ChIP™ A/G Chromatin Immunoprecipitation Kit (62 citations) Magna ChIP A kit (24 citations) Magna ChIP Assay Kit (22 citations) Millipore ChIP Assay Kit (12 citations) EZ‐Magna CHIP™ HiSens kit (10 citations)

270 protocols using chip kit

1

ChIP Assay Protocol with Antibody Validation

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A Millipore ChIP kit and Diagenode ChIP kit was used for ChIP assay. DNA-protein complexes were crosslinked with formaldehyde at a final concentration of 1%. Sonicated extracts were precleared and incubated with Abs specific to Ezh2, H3K27me3, or nonspecific anti-IgG. The immunoprecipitated DNA was quantified by real-time quantitative PCR. The primer sequences are listed in Supplementary Table 2. The Ab information is listed in Supplementary Table 3.
He S., Liu Y., Meng L., Sun H., Wang Y., Ji Y., Purushe J., Chen P., Li C., Madzo J., Issa J.P., Soboloff J., Reshef R., Moore B., Gattinoni L, & Zhang Y. (2017). Ezh2 phosphorylation state determines its capacity to maintain CD8+ T memory precursors for antitumor immunity. Nature Communications, 8, 2125.
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2

Th2 Cell Modulation in Allergy

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The antibodies of HDAC1, pSTAT6, p300, pp300, STAT3, pSTAT3, H3K4ac, RNA polymerase II, shRNA kits of p300 and STAT3 were from Santa Cruz Biotech (Santa Cruz, CA). The fluorochrome-labeled antibodies of Foxp3, CD4, IL-10, CD19 and IgE were from BD Biosciences (Franklin Lakes, NJ). The biotinylated IgE antibody was from Abcam (Cambridge, MA). Magnetic cell sorting kits were from Miltenyi Biotech (San Diego, CA). The house dust mite vaccine was from Wowu Biotech (Hangzhou, China). Clostridium butyricum was from Shenzhen Kexing Biotech (Shenzhen, China). The Der p 1 protein was from Dr. Zhijiang Liu (Shenzhen University, China). PCI-32765 was purchased from Chem Blink (Shanghai, China). Reagents for real time RT-PCR and Western blotting were from Invitrogen (Carlsbad, CA). Protein G, ChIP kit and butyrate sodium were from Sigma Aldrich (St. Louis., MO).
Liao H.Y., Tao L., Zhao J., Qin J., Zeng G.C., Cai S.W., Li Y., Zhang J, & Chen H.G. (2016). Clostridium butyricum in combination with specific immunotherapy converts antigen-specific B cells to regulatory B cells in asthmatic patients. Scientific Reports, 6, 20481.
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3

Evaluating RNA Polymerase II and snRNP70 Binding

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The ChIP kit was purchased from Sigma and protocol followed as per manufacturer’s instruction. RNA polymerase II antibody and SNRNP70 antibody were purchased from Novus, and IgG antibody was included in kit (Sigma). Following cross-linking, cell lysate (10%) was removed for input sample. Immunoprecipitation was performed with 2μg RNA polymerase II antibody, SNRNP70 antibody (positive control) or IgG antibody (as negative control). SYBR Green Real-time qPCR was performed as described above using GAS5 primers and primers for U1 RNA, the binding partner for the positive control SNRNP70. The binding events and specificity (fold enrichment) was calculated using Excel™ template for ChIP from manufacturer.
Tong H.H., Blue L.E., James M.A., Chen Y.P, & DeMaria T.F. (2000). Evaluation of Phase Variation of Nontypeable Haemophilus influenzae Lipooligosaccharide during Nasopharyngeal Colonization and Development of Otitis Media in the Chinchilla Model. Infection and Immunity, 68(8), 4593-4597.
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4

Vascular Barrier Integrity Assay

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The LPS, FITC-labeled dextran, calcitriol and ChIP kit were purchased from Sigma Aldrich (St. Louis., MO). The antibodies of VDR, ZO-1, claudin-5, occludin, HDAC11, acH3, acH4, shRNA kits of VDR and HDAC11 were purchased from Santa Cruz Biotech (Santa Cruz, CA). The reagents for Western blotting and RT-qPCR were purchased from Invitrogen (Carlsbad, CA). The reagents used in this study contained <0.2U endotoxin/10 μg reagents as assessed using the Limulus assay (Limulus amebocyte lysate QCL 1000, Bio Whittaker, Walkersville, MD, USA).
Liu F.H., Li S.S., Li  X.X., Wang  S., Li  M.G., Guan L., Luan T.G., Liu Z.G., Liu Z.J, & Yang P.C. (2017). Vitamin D3 induces vitamin D receptor and HDAC11 binding to relieve the promoter of the tight junction proteins. Oncotarget, 8(35), 58781-58789.
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5

Identification of PAX7 Binding Sites

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Putative PAX7 consensus sequences identification were identified using the match tool of Transfac. The ChIP assay was performed by the use of ChIP Kit (Sigma-Aldrich, Milan, IT; cat. no. CHP1) following the manufacturer’s instructions. The genomic DNA was sonicated, immunoprecipitated overnight at 4 °C with an antibody directed against PAX7 validated for ChIP (anti-PAX7 used at 2 µg/ml; Millipore, clone 2F12H4). Immunoprecipitated DNA was then analyzed by qPCR (40–45 cycles) using primers amplifying each of PAX7-rich regions identified (Supplemental Table 3). In addition, for each primer pair, non-immunoprecipitated DNA was used as input for normalization. All ChIP assays were performed in triplicate for at least three different biological preparations.
Iannotti F.A., Pagano E., Guardiola O., Adinolfi S., Saccone V., Consalvi S., Piscitelli F., Gazzerro E., Busetto G., Carrella D., Capasso R., Puri P.L., Minchiotti G, & Di Marzo V. (2018). Genetic and pharmacological regulation of the endocannabinoid CB1 receptor in Duchenne muscular dystrophy. Nature Communications, 9, 3950.
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6

Molecular Techniques in Cancer Research

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The antibodies of Trib1, HDAC1, pHDAC1, p53, and shRNA kits of Trib1 and HDAC1 were from Santa Cruz Biotech (Shanghai, China). The Annexin V kit, protein G, butyrate sodium and Chip kit were from Sigma Aldrich (Shanghai, China). The reagents for RT-qPCR and Western blotting were from Invitrogen (Shanghai, China).
Tang B., Wu W., Zhang Q., Sun Y., Cui Y., Wu F., Wei X., Qi G., Liang X., Tang F., Li Y, & Fan W. (2015). Inhibition of tribbles protein-1 attenuates radioresistance in human glioma cells. Scientific Reports, 5, 15961.
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7

ChIP-qPCR Analysis of H3K27ac in A549 and H1299 Cells

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ChIP kit (Sigma-Aldrich, Missouri, USA) was used for ChIP assay. A549 and H1299 cells were cross-linked with 1% formaldehyde (Sigma-Aldrich) for 10 min at room temperature. Cells were lysed with lysis buffer, and then sonicated for 30 min to generate DNA fragments with size of 100–1000 bp. Then, cells were immunoprecipitated with anti-H3K27ac (1:200, #4353, Cell Signaling, MA, USA) or anti-IgG (1:200, #3900, Cell Signaling). The purified DNA fragments were quantified by qRT-PCR. The primers of SEs for AC074117.1 were as follows: E1, forward: 5ʹ-CAGCATCCCAAGGCAGAAGA-3ʹ, reverse: 5ʹ-GTGTACCCAACAGCTCCGAA-3ʹ. E2, forward: 5ʹ-CAAAAACCAGTCAGGCGTGG-3ʹ, reverse: 5ʹ- TCCTCCCTCTCCCTTCTTCG-3ʹ.
Li M., Yang B., Li X., Ren H., Zhang L., Li L., Li W., Wang X., Zhou H, & Zhang W. (2021). Identification of Prognostic Factors Related to Super Enhancer-Regulated ceRNA Network in Metastatic Lung Adenocarcinoma. International Journal of General Medicine, 14, 6261-6275.
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8

Comprehensive Mouse TAFA4 Immunoassay Protocol

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Mouse TAFA4 ELISA kit was purchased from Kanglang Biotech (Shanghai, China). Anti-mouse FPR1 (PAB0284) Ab were purchased from Kemi Biotech (Shanghai, China). Anti-mouse TAFA4 Ab (PA569319) was purchased from Shanghai Saimofeishier Biotech (Shanghai, China). Recombinant mouse TAFA4 protein was purchased from CUSABio (Wuhan, China). MyD88 (sc-74532, E-11), AKT (sc-81434, 5C10; sc-514032, C-11), CD11c (sc-398708, D-8, AF594), IL-10 (sc-57245, 2G101H7, AF488), CD3 (sc-20047, PC3/188 A, AF488), CD4 (sc-19641, MT310, AF594), CD49b (sc-53353, HAS-4, AF546), LAG3 (sc-32750, C9B7W, AF648) were purchased from Santa Cruz Biotech (Santa Cruz Biotech (Santa Cruz, CA). Ki-67 Ab (ab16667, SP6, AF488) was purchased from abcam (Cambridge, MA). ELISA kits of sIgE, Mcpt1, EPX, IL-4, IL-5, IL-10, IL-13 were purchased from CRK Pharma (Wuhan, China). The ChIP kit was purchased from Sigma Aldrich (St. Louis., MO). Reagents and materials for RT-qPCR and Western blotting were purchased from Invitrogen (Carlsbad, CA).
Qiu S., Luo X., Mo L., Zhang S., Liao Y., Guan L., Yang L., Huang Q., Liu D, & Yang P. (2022). TAFA4-IL-10 axis potentiate immunotherapy for airway allergy by induction of specific regulatory T cells. NPJ Vaccines, 7, 133.
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9

Comprehensive Immunology Assay Protocol

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The ELISA kits of IL-4 (Cat#M4000B), IL-9 (DY409), IFN-γ (MIF00) and recombinant IL-9 were purchased from R&D Systems (Minneapolis, MN). SEB (s4881), garcinol (g5713), Annexin V kit, ChIP kit and protein G agarose beads were purchased from Sigma Aldrich (St. Louis., MO). The fluorochrome labeled antibodies of CD11c (561241), CD44 (563058), CD3 (561824), CD4 (563933), IL-9 (D9302C12), IL-4 (553047), IL-17 (560489) and IFN-γ (557998) were purchased from BD Biosciences (Franklin Lakes, NJ). The reagents for RT-qPCR and Western blotting were purchased from Invitrogen (Carlsbad, CA). Antibodies of H3K4ac, IL-9 (7923), p300 (585), pp300 (130210) and PU.1 (390405) were purchased from Biomart (Shanghai, China). The immune cell isolation kits were purchased from Miltenyi Biotech (San Diego, CA). The APAAP reagent kit was purchased from the Gukang Biotech (Guangzhou, China). The shRNA kits of VDR and p300 were purchased from Santa Cruz Biotech (Santa Cruz, CA).
Zheng H., Yang B., Xu D., Wang W., Tan J., Sun L., Li Q., Sun L, & Xia X. (2016). Induction of specific T helper-9 cells to inhibit glioma cell growth. Oncotarget, 8(3), 4864-4874.
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10

Identification of FOXM1 Transcriptional Targets

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DNAStar Lasergene 8 Suite Sequence Builder and Genequest software was used to determine the transcription factor binding sites surrounding 1 kb upstream and 1 kb downstream of the transcription start points (TSPs) in the human genome database in GenBank. Computer-assisted search for the typical FOXM1 binding motif which is tandem repeats of ‘TAACA’, the atypical binding motifs such as ‘AACA’ and ‘TAAC, or their complements, was conducted to uncover the putative FOXM1 binding sites. Chromatin immunoprecipitation (ChIP) was done using ChIP kit procured from Sigma-Millipore, USA and modified as described previously (22 (link)). Immunoprecipitation was performed using anti-FOXM1 monoclonal antibodies (SCBT)and anti-IgG (SCBT) or ‘no antibody’ blanks were used as negative controls. Based on our in-silico analysis, the putative FOXM1 binding sites were identified and qPCR was performed as described above by using primers listed (Supplementary Table-4) surrounding the FoxM1 binding sites. AURKB promoter was selected as a positive control for FOXM1 as it was identified as direct target of FOXM1 for transcription (23 (link)). β-actin (ACTB) promoter sequence which do not have FOXM1 binding sequences was used as the negative control.
Parashar D., Nair B., Geethadevi A., George J., Nair A., Tsaih S.W., Kadamberi I.P., Nair G.K., Lu Y., Ramchandran R., Uyar D.S., Rader J.S., Ram P.T., Mills G.B., Pradeep S, & Chaluvally-Raghavan P. (2020). Peritoneal Spread of Ovarian Cancer harbors Therapeutic Vulnerabilities Regulated by FOXM1 and EGFR/ERBB2 Signaling. Cancer research, 80(24), 5554-5568.
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