Hypericin was purchased from Thermo Fisher Scientific (Karlsruhe, Germany). 1,2-distearoyl-sn-glycero-3- phosphatidyl-choline (DSPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphatidyl-choline (DPPC), were a gift from Lipoid GmbH (Ludwigshafen, Germany). A polar lipid fraction containing tetraether lipids caldarchaeol (GDGT ∼10% of the extract) and calditoglycerocaldarchaeol (GDNT ∼90% of the extract) was extracted from the biomass of S. acidocaldarius (SIT Rosenhof GmbH, Heiligenstadt, Germany) as previously mentioned (Engelhardt et al., 2017 ). (2-hydroxypropyl)-β-cyclodextrin (HPβCD) and all other chemicals and solvents used were of analytical grade and were purchased from Sigma-Aldrich (Taufkirchen, Germany).
2 hydroxypropyl β cyclodextrin hpβcd
Manufactured by Merck Group
Sourced in United States, Germany
2-hydroxypropyl-β-cyclodextrin (HPβCD) is a cyclic oligosaccharide derived from starch. It is used as a solubilizing and stabilizing agent in various pharmaceutical and chemical applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Merck Group (2 mentions)
Hplc grade acetonitrile, by Merck Group (2 mentions)
Hypericin, by Thermo Fisher Scientific (1 mentions)
1 2 dipalmitoyl sn glycero 3 phosphatidylcholine, by Lipoid (1 mentions)
Celltiter 96 aqueous one solution cell proliferation assay system, by Promega (1 mentions)
Filipin 3, by Merck Group (1 mentions)
2 hydroxypropyl γ cyclodextrin hpγcd, by Merck Group (1 mentions)
Glassy carbon electrode, by CH Instruments (1 mentions)
2 nitrophenyl octyl ether o npoe, by Merck Group (1 mentions)
Tetrahydrofuran thf, by BD (1 mentions)
Sunflower oil, by Spectrum Chemical (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Htb 148, by American Type Culture Collection (1 mentions)
Pmscv pig, by Addgene (1 mentions)
Bafilomycin, by Cayman Chemical (1 mentions)
Pd123319, by Abcam (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
21 protocols using 2 hydroxypropyl β cyclodextrin hpβcd
1
Hypericin-loaded Liposomal Formulation
2
Cholesterol Trafficking Inhibition Assay
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Cell culture media and reagents were purchased from Thermo Fisher Scientific (Waltham, MA, USA). These include Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin. A CellTiter 96 Aqueous One Solution Cell Proliferation Assay System was purchased from Promega (Madison, WI, USA). U18666A, Filipin III, 2-hydroxypropyl-β-cyclodextrin (HPβCD) and 2-hydroxypropyl-γ-cyclodextrin (HPγCD) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Primary antibodies used in this study are as follows: rabbit polyclonal antibody for mCherry (Abcam ab183628); rabbit monoclonal antibody for GAPDH (Cell Signaling #5174).
3
Potentiometric Determination of Midazolam
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All reagents and chemicals used were of analytical grade and the water was bi-distilled. 3,5 bis(trifluoromethyl)aniline, high molecular weight polyvinyl chloride (PVC), Potassium tetrakis (4-chlorophenyl)borate (KTCPB), calix-[8]-arene (CX8), calix-[6]-arene (CX6), calix-[4]-arene (CX4), 2-hydroxypropyl β-cyclodextrin (HP-β-CD), 2-nitrophenyl octyl ether (o-NPOE) and midazolam (MDZ) United States Pharmacopeia (USP) Reference Standard were purchased from Sigma-Aldrich (Steinheim, Germany). Tetrapentylammonium bromide ((Pen)4N+Br−) was purchased from Merck (Darmstadt, Germany) while tetrahydrofuran (THF) was obtained from BDH (Poole, England). Acetate buffer pH 4 was used. Midathetic® ampoule (5 mg/mL) was manufactured by Amoun Pharmaceutical Co. S.A.E. (Batch No. 182828). Glassy carbon electrodes were used as the electrode (3 mm diameter, CH Instruments, Texas, USA).
4
Synthesis and Formulation of Novel Pyrazolo-pyrimidine Compounds
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G7883 (5-(4-cyclohexylphenyl)-3-[3-(fluoromethyl)azetidine-1-carbonyl]-2-(3-methyl pyrazin-2-yl)-4H-pyrazolo[1,5-a]pyrimidin-7-one) and G6893 ([rac-(3R,4S)-4-methyltetra hydrofuran-3-yl]N-[8-amino-7-fluoro-6-(8-methyl-2,3-dihydro-1H-pyrido[2,3-b][1,4] oxazin-7-yl)-3-isoquinolyl]carbamate) compounds were synthesized at Genentech (South San Francisco, CA, USA). Polyethylene glycol 400 UPS grade, sunflower oil, corn oil, and polysorbate 80 NF grade were from Spectrum™ Chemical (New Brunswick, NJ, USA). Organic solvents (Dimethyl Sulfoxide and Ethanol), Methyl cellulose M6385 A15 LV, and (2-Hydroxypropyl)-β-cyclodextrin (HP-β-CD) were from Sigma-Aldrich (St. Louis, MO, USA). Micro-infusion pump iPRECIO SMP-300 was purchased from ALZET (Cupertino, CA, USA).
5
TFEB Regulation in α-Synuclein Model
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2-hydroxypropyl-β-cyclodextrin (HPβCD) and cholesterol were purchased from Sigma-Aldrich, bafilomycin was from Cayman Chemical, and DAPI nuclear stain was from Enzo Life Sciences. TFEB siRNA (Cat. No. SI00094969) and control siRNA (Cat. No. 1027280) were purchased from Qiagen. pMSCV-PIG, gag-pol, and VSVG plasmids were from Addgene and TFEB-3XFLAG plasmid was a generous gift from Dr. Marco Sardiello (Baylor College of Medicine, Houston, TX)
H4 cells stably transfected for the expression of α-syn-EmGFP (H4/α-syn-GFP) were generated as previously described [34 (link),35 (link)]. Human H4 neuroglioma (HTB-148, ATCC), H4/α-syn-GFP, and HEK-293T cells (CRL-11268, ATCC) were cultured in high glucose DMEM (Fisher Scientific) supplemented with 10% fetal bovine serum and 1% PSQ, and maintained at 37°C and 5% CO2.
H4 cells stably transfected for the expression of α-syn-EmGFP (H4/α-syn-GFP) were generated as previously described [34 (link),35 (link)]. Human H4 neuroglioma (HTB-148, ATCC), H4/α-syn-GFP, and HEK-293T cells (CRL-11268, ATCC) were cultured in high glucose DMEM (Fisher Scientific) supplemented with 10% fetal bovine serum and 1% PSQ, and maintained at 37°C and 5% CO2.
6
Inclusion Compound of HP-β-CD and Alamandine
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The inclusion compound HP-β-CD/alamandine was prepared for in vivo experiments as previously described [14 (link)]. (2-Hydroxypropyl)-β-Cyclodextrin (HP-β-CD) was purchased from Sigma-Aldrich (332,607, average Mw ~ 1,460, USA). Inclusion complex between the alamandine (TGpeptide, Nanjing, China) and the HP-β-CD was prepared by the freeze-drying process using the 1:1 molar ratio. An aqueous solution, using Milli-Q® water, of host and guest molecules was stirred for 3 h to ensure that equilibrium had been reached. Then, the solution was frozen in liquid nitrogen and lyophilized (LC-18 N-50 A Freeze-Dryer, LICHEN, China) for 48 h to obtain the solid inclusion complex and stored at -20℃ for later use. PD123319, the MrgD antagonist, was purchased from Abcam and dissolved in distilled water for oral gavage.
7
Valorization of Citrus Lemon Waste
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Citrus lemon waste was kindly supplied by Università Mediterranea of Reggio Calabria (Italy), with a moisture content of about 15% by weight. Glacial acetic acid (HAc), isopropanol, ethanol, acetone, 2-Hydroxypropyl-β-cyclodextrin (HP-β-CD), Folin–Ciocalteu (F–C) reagent, gallic acid, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) are all analytical grades purchased by Sigma-Aldrich (Darmstadt, Germany).
8
Curcumin-Loaded Lipid Nanoparticles
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Curcumin was kindly gifted by Guangzhou Phytochem Sciences Inc., China. Lecithin was brought from Fischer Scientific, USA. Cholesterol, stearylamine (SA), poloxamer 188 (PLX 188) and 2-hydroxypropyl-β-cyclodextrin (HPβCD) and dialysis tubing cellulose membrane (molecular weight cutoff 14,000 g/mol) were procured from Sigma-Aldrich, St. Louis, USA. Methanol and acetonitrile, HPLC grade, were bought from Sigma-Aldrich, UK.
The rest of the reagents were of analytical grade and used as received.
The rest of the reagents were of analytical grade and used as received.
9
Hesperidin and Hesperetin Permeability
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All materials including the tested compounds—hesperidin (Hed, purity > 80%) and hesperetin (Het, purity > 95%)—and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) were supplied by Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA), except for dimethyl sulfoxide (DMSO), potassium persulfate czda and sodium hydroxide (Avantor Performance Materials Poland S.A., Gliwice, Poland), acetic acid 98–100% and sodium chloride (POCH, Gliwice, Poland), the analytical weighed amount of HCl 1 N (Chempur, Piekary Slaskie, Poland) and sodium dihydrogen phosphate (PanReac AppliChem ITW Reagents, Darmstadt, Germany), and methanol of an HPLC grade (J. T. Baker, Center Valley, PA, USA). High-quality pure water was prepared using a Direct-Q 3 UV purification system (Millipore, Molsheim, France; model Exil SA 67120). Prisma HT, GIT/BBB lipid solution, and acceptor sink buffer were supplied by Pion Inc. (Forest Row, East Sussex, United Kingdom).
10
Synthesis and Characterization of BMK-20113
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BMK-20113 was synthesized in our lab and its structure and activity were described in our previous report.40 (link) Emulgade® SE-PF, tetradecyl tetradecanoate (Cetiol® MM, myristyl myristate), polyethylene glycol (PEG)-15 hydroxystearate (Solutol® HS 15), and PEG-12 cetostearyl ether (Eumulgin® B1, ceteareth-12) were kindly provided by BASF SE (Dusseldorf, Germany). 2-Hydroxypropyl-β-cyclodextrin (HP-β-CD) was purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Carbopol 980 NF was purchased from Shanghai Chineway Pharmaceutical Technology (Shanghai, People’s Republic of China). The water used in the sample preparation, high-performance liquid chromatography (HPLC), and liquid chromatography-mass spectrometry (LC-MS/MS) procedures and the isopropyl alcohol used in the HPLC procedure were of HPLC grade and were purchased from Thermo Fisher Scientific (Waltham, MA, USA). All other reagents were of analytical grade and were used as supplied.
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