M1000

Manufactured by Tecan

Sourced in Switzerland, Austria, United States

The M1000 is a microplate reader designed for high-performance absorbance measurements. It features a wide wavelength range, flexible microplate formats, and fast measurement times. The core function of the M1000 is to accurately measure the absorbance of samples in microplates.

Automatically generated - may contain errors

Lab products found in correlation

Microplate reader, by Tecan (6 mentions) Homogenizing buffer, by Thermo Fisher Scientific (6 mentions) Quantigene 2.0 assay, by Thermo Fisher Scientific (6 mentions) Proteinase k, by Thermo Fisher Scientific (6 mentions) Dual luciferase assay kit, by Promega (5 mentions) Rnalater, by Merck Group (5 mentions) Dual glo luciferase assay system, by Promega (4 mentions) Bright glo luciferase assay kit, by Promega (3 mentions) Substrate solution, by Promega (3 mentions) Veritas luminometer, by Promega (3 mentions) Genesys 150 uv vis spectrometer, by Thermo Fisher Scientific (2 mentions) M1000 plate reader, by Tecan (2 mentions) Cyto id autophagy detection kit, by Enzo Life Sciences (2 mentions) Probe tip, by Sonics (2 mentions) Ir783, by Merck Group (2 mentions) Uv star, by Tecan (2 mentions) M1000 microplate reader, by Tecan (2 mentions) Prism 8, by GraphPad (2 mentions) Tmb substrate solution, by Thermo Fisher Scientific (2 mentions) Gel filtration standard, by Bio-Rad (2 mentions)

189 protocols using m1000

Encapsulation of Ovalbumin in Amino-Functionalized Mesoporous Silica Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

For loading of OVA into AEP-MSNs, OVA (0.5 mL, 0.5 mg/mL‚ 1 mM PB) and AEP-MSN (0.5 mL, 2 mg/mL, 1 mM PB) were mixed and incubated in Eppendorf mixer (400 rpm, 25°C, Nijmegen, the Netherlands) for different time periods (0, 0.5, 1, 2, 4, 8 and 24 h). After incubation, the suspensions were centrifuged and the encapsulation efficiency (EE%) of OVA was determined by measuring the difference in its intrinsic fluorescence intensity with a plate reader (Tecan M1000, Männedorf, Switzerland) (excitation wavelength = 280 nm and emission wavelength = 320 nm) in the supernatant before and after the encapsulation.
To determine the maximum loading capacity (LC%) of OVA in AEP-MSNs, the AEP-MSNs (2 mg/mL) were mixed with different initial concentrations of OVA (ranging from 0.25, 0.5, 1, 1.5, 2 to 3 mg/mL) and incubated in an Eppendorf mixer (400 rpm, 25°C) for 0.5 h. Next, the suspensions were centrifuged at 9000 g for 5 min. The EE% of OVA was determined by measuring the difference in their intrinsic fluorescence intensity in the supernatant before and after the encapsulation with a plate reader (Tecan M1000).
The EE% and LC% were calculated as below:

EE % = \frac{t_{ova} - f_{ova}}{t_{ova}} \times 100 %

LC % = \frac{t_{ova} - f_{ova}}{OVA loaded AEP - MSNs} \times 100 %

Where t_ova represents the total content of OVA, and f_ova is the content of free OVA (OVA in the supernatant).

Tu J., Du G., Reza Nejadnik M., Mönkäre J., van der Maaden K., Bomans P.H., Sommerdijk N.A., Slütter B., Jiskoot W., Bouwstra J.A, & Kros A. (2017). Mesoporous Silica Nanoparticle-Coated Microneedle Arrays for Intradermal Antigen Delivery. Pharmaceutical Research, 34(8), 1693-1706.

+ Open protocol

+ Expand

Quantitative β-Galactosidase Assay in E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasmids with lacZ fusion reporters were transformed into VH1000 E. coli cells [Δ(lacI–lacZ)]. Fresh colonies were inoculated into MOPS RDM with 0.2% glucose for overnight growth. On the next day, 10 μl of overnight culture was mixed with 190 μl RDM in a 96-well plate and shaken at 600 rpm at 37°C for 2–3 h until reaching mid-exponential phase. The cells were resuspended using a multichannel pipettor before recording apparent OD₆₀₀ values on a plate reader (Tecan M1000). 10 μl A portion (10 μl) of cells was permeabilized by thoroughly mixing with 110 μl lysis buffer (60 mM Na₂HPO₄, 40 mM NaH₂PO₄, 10 mM KCl, 1 mM MgSO₄, 50 mM β-mercaptoethanol, 0.005% SDS) and 4 μl chloroform at room temperature. The β-galactosidase reaction was initiated by addition of 24 μl ortho-Nitrophenyl-β-galactoside (ONPG, 4 mg/ml) and stopped by addition of 60 μl 1M Na₂CO₃ at the reaction time (t, min) recorded. Absorbance at 420 nm (A₄₂₀) and 550 nm (A₅₅₀) was measured using a Tecan plate reader (M1000). β-galactosidase activity was calculated using the following formula.
When used, bicyclomycin was added to cultures to 20 μg/ml as the final concentration.

Bao Y., Cao X, & Landick R. (2024). RNA polymerase SI3 domain modulates global transcriptional pausing and pause-site fluctuations. Nucleic Acids Research, 52(8), 4556-4574.

+ Open protocol

+ Expand

Serum LDH and cTnI Assay Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum LDH and cTnI were assayed using related commercial assay kits (C0016, Beyotime Institute of Biotechnology, China, and E-EL-M1203c, Elabscience Biotechnology Co. Ltd., China, respectively) according to the manufacturers' instructions. For LDH detection, the cell supernatant or serum was incubated with prepared LDH working solution in the dark for 30 minutes at room temperature. Absorbance at 490 nm was measured using a microplate reader (M1000, TECAN, Austria GmbH, Austria). For the cTnI assay, serum was added into the well plate precoated with the mouse cTnI specific antibody; then, a biotinylated antibody specific for cTnI and avidin-horseradish peroxidase (HRP) conjugate were added successively to each well plate and incubated. After free components were washed away, the substrate solution was added into each well. Finally, stop solution was added to terminate the enzyme-substrate reaction, and then, the color turns yellow. The optical density was measured spectrophotometrically at 450 nm using a microplate reader (M1000, TECAN, Austria GmbH, Austria).

Chang L., Wang Z., Ma F., Tran B., Zhong R., Xiong Y., Dai T., Wu J., Xin X., Guo W., Xie Y., Mao Y, & Zhu Y.Z. (2019). ZYZ-803 Mitigates Endoplasmic Reticulum Stress-Related Necroptosis after Acute Myocardial Infarction through Downregulating the RIP3-CaMKII Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2019, 6173685.

+ Open protocol

+ Expand

Quantifying VIC Metabolic Activity, Proliferation, and Phenotype

Check if the same lab product or an alternative is used in the 5 most similar protocols

VICs were seeded in 96 well plates as described earlier and cultured for 72 h. ATP amount was measured as an indicator of metabolic activity and cell viability using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI). The CellTiter-Glo Reagent was added to VICs growing in a 96 well plate and placed on a shaker for 2 min for cell lysis. Following a 10 min incubation at room temperature, luminescence was recorded using a microplate reader (Tecan M1000, Mannedorf, Switzerland). Proliferation was assessed via incorporation and detection of BrdU. VICs were seeded in 24 well plates, cultured for 64 h, and then incubated with BrdU for 8 h at the manufacturer-recommended dilution (BrdU Cell Proliferation Assay Kit, Cell Signaling Technology, Danvers, MA). Cells were then fixed, BrdU detected with an HRP-linked secondary antibody, and proliferation evaluated via measurement of absorbance at 450 nm (Tecan M1000) per kit instructions. Alpha smooth muscle actin (αSMA) was detected by flow cytometry 72 h after seeding. Briefly, VICs (1 × 10⁵) were resuspended in 100 μL PBS, incubated for 30 min on ice with an antibody against αSMA (Clone 1A4, Sigma Aldrich), labeled with a FITC-conjugated anti-mouse secondary antibody (Thermo Fisher), and fixed in 150 μL 0.05% formaldehyde, before 10,000 events were acquired on a BD FACSCaliber flow cytometer.

Henderson C.J., Smith A.G., Ure J., Brown K., Bacon E.J, & Wolf C.R. (1998). Increased skin tumorigenesis in mice lacking pi class glutathione S-transferases. Proceedings of the National Academy of Sciences of the United States of America, 95(9), 5275-5280.

+ Open protocol

+ Expand

High-Throughput Cell Viability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 29

A549 cells were plated into wells of a 384-well assay plate at 250 cells/well. Cells were treated with dose curves of staurosporine or doxorubicin for 72 hours. After 72 hours, 50 uM PBI 4586 was added to the cell culture and incubated at room temperature for 30 minutes. LDR and 10 mM D-cysteine was added to the reaction and incubated at room temperature for 20 minutes. Luminescence was measured on a Tecan M1000.

The results illustrate the use of the method described to rapidly identify and characterize compounds affecting cell viability (FIG. 12).

US09951372B2. Compounds and methods for assaying redox state of metabolically active cells and methods for measuring NAD(P)/NAD(P)H (2018-04-24). PROMEGA CORPORATION [US]. Inventors: Wenhui Zhou [US], Jolanta Vidugiriene [US], Helene A. Benink [US], James J. Cali [US], Sarah Duellman [US], Dieter Klaubert [US], Donna Leippe [US], Martha O'Brien [US], John Shultz [US], Mary Sobol [US].

+ Open protocol

+ Expand

Quantifying Dad Absorbance Spectra

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dad was dissolved in MilliQ water and 100 uL of 2 M sodium hydroxide to make a to a 5 mM starting stock. Samples were individually diluted to the desired concentration using 50:50 acetonitrile to phosphate buffered saline (CH₃CN/PBS) in triplicate. The absorbance spectrum was measured on a ThermoScientific Genesys 150 UV-Vis spectrometer, using 50:50 CH₃CN/PBS as a blank. These absorbances were also measured using a Tecan M1000 plate reader with acridone as a standard.

Jones C.M., Petersson G.A, & Petersson E.J. (2021). Synthesis and characterization of fluorescent amino acid dimethylaminoacridonylalanine. ARKIVOC : free online journal of organic chemistry, 2021, 97-109.

+ Open protocol

+ Expand

Quantifying Dad Absorbance Spectra

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Fluorescence Polarization Nanoparticle Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols

The application of FP is based on the assumption that binding of the fluorescence-labeled molecule to nanoparticles decreases its molecular rotation in solution which can be detected by FP. NQA was N-terminally labeled with FAM and purified. Then a constant amount of FAM-labeled peptide (about 20 nmol/L) was incubated with suspensions of nanoparticles in water at a range of concentrations. FP was measured using a Tecan M1000 microplate reader (Tecan, Swiss), and values corrected by subtracting the value corresponding to the labeled peptide in water.

Han J., Sun Y., Hou J., Wang Y., Liu Y., Xie C., Lu W, & Pan J. (2015). Preliminary investigations into surface molecularly imprinted nanoparticles for Helicobacter pylori eradication. Acta Pharmaceutica Sinica. B, 5(6), 577-582.

+ Open protocol

+ Expand

SMAC-mCherry Mitochondrial Release Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

SMAC-mCherry release assays were carried out as previously described (Niu et al., 2017 (link)). Briefly, BAX^-/-/BAK^-/- baby mouse kidney (BMK) cells stably expressing a fusion protein comprised of the mitochondrial import peptide of SMAC (amino acids 1–58) fused to the amino-terminus of the fluorescent protein mCherry were lysed by nitrogen cavitation at 150 psi for 10 min on ice in buffer containing 20 mM HEPES (pH 7.2), 250 mM sucrose, 150 mM potassium chloride, 1 mM EDTA, 1 X protease inhibitor cocktail. Nuclei and cell debris were removed by centrifugation of the lystate at 2000 g for 4 min at 4 °C. Heavy membranes containing mitochondria were obtained by centrifuging the supernatant at 13,000 g for 10 min at 4 °C and washing the pellet once in lysis buffer. Membrane fractions (0.2 mg/ml protein) were incubated with desired BCL-2 family proteins in 250 μL volume and incubated for 30 min at 37 °C. Mitochondria were then pelleted by centrifugation at 13,000 g for 10 min. The release of SMAC-mCherry was measured as fluorescence intensity using a Tecan M1000 microplate reader and comparing fluorescence intensity between the supernatant and pellet fractions. The percentage release of SMAC-mCherry was calculated as [Fsupernatant /(Fsupernatant +Fpellet)] x 100.

Pemberton J.M., Nguyen D., Osterlund E.J., Schormann W., Pogmore J.P., Hirmiz N., Leber B, & Andrews D.W. (2023). The carboxyl-terminal sequence of PUMA binds to both anti-apoptotic proteins and membranes. eLife, 12, e88329.

+ Open protocol

+ Expand

Oxidative Stress Response in Yeast

Check if the same lab product or an alternative is used in the 5 most similar protocols

Yeast overnight cultures were diluted to an OD₆₀₀ of 0.2 in 5 ml SD-Ura media and grown for about 3.5 h at 30°C until an OD₆₀₀ of 0.5–0.6 was reached. Cells were centrifuging at 3,000 × g for 3 min and pellets were resuspended in 700 μl SD-Ura media pre-warmed to 37°C without or with 0.5 mM H₂O₂. 200 μl of each culture was pipetted in triplicates into a 96-well plate. The plate was covered with a breathable microplate sealing film and incubated for 48 h at 37°C in a Tecan M1000 plate reader measuring the OD₆₀₀ every 10 min.

Ulrich K., Farkas Á., Chan O., Katamanin O., Schwappach B, & Jakob U. (2022). From Guide to Guard – Activation Mechanism of the Stress-Sensing Chaperone Get3. Molecular cell, 82(17), 3226-3238.e7.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!