Verikine hs mouse interferon beta elisa kit

Mouse blood was collected from the submandibular vein and clotted at room temperature for 20 min before centrifugation at 2000 × g for 10 min. The serum was collected and stored at −80 °C. VeriKine-HS Mouse Interferon-Beta ELISA Kit (Pbl Assay Science) was used to determine IFNB1 levels in serum, according to the manufacturer’s instructions.

4T1 cells were seeded into 28.2 cm² dishes and irradiated with single doses of 4, 6, 8, 12, 16, 20, and 24 Gy or repeated doses of 8, 6 and 4 Gy on 3, 4 and 6 consecutive days, respectively. Cell-free supernatant was collected 24 hours after the last dose and IFNB1 was measured using the VeriKine-HS Mouse Interferon Beta ELISA Kit (PBL Assay Science), according to the manufacturer’s instruction. 50 µL of undiluted supernatant or recombinant IFNB1 standards were processed in technical triplicate wells. 450 nm optical density was measured using a FlexStation 3 plate reader (Molecular Devices). Concentrations were normalized by the number of viable cells.