Buspirone hydrochloride

(-)-Nicotine free base (CAS no. 54-11-5) and (-)-cotinine free base (CAS no. 486-56-6) were purchased from Sigma-Aldrich® (St. Louis, MO, United States). (±)-Anatabine citrate (purity 98.92% by HPLC) was purchased from Concept Life Sciences (Manchester, UK). (±)-Anatabine free base (purity >95% by HPLC) used for the SmartCube^® study was a generous gift from Indena® S. p.A. (Milan, Italy) (Rossia et al., 2018 (link)). (±)-Anatabine free base used for the zebrafish NTT was custom synthesized by WuXi AppTec (purity ≥95%; Shanghai, China). PNU282987 (CAS no. 711085-63-1) and buspirone hydrochloride (CAS No. 33386-08-2) were purchased from Tocris Bioscience (Bio-Techne®, Minneapolis, MN, United States). AZD1446 (CAS no. 1025007-04-8) was purchased from Key Organics Limited (Cornwall, UK).

[Arg8]-Vasotocin acetate salt (the non-mammalian homologue of AVP) was purchased from Alfa Aesar (Cat. no. J66551) and buspirone hydrochloride was purchased from Tocris (Cat. no. 0962). AVP was injected intraperitoneally 10 min before shoaling was measured. Body weight was measured to calculate the amount to inject. We injected 5 μg/gbw AVP dissolved in 0.9% saline (Oxoid, Cat. no. BO0334C) using a Hamilton syringe (Sigma, Cat. no. 80200). This concentration was chosen according to^{33 (link)}. Control animals were given a sham injection of saline before behaviour was measured. Buspirone was applied by acute immersion in system water containing 25 µM drug for 1 h. The concentration was chosen according to previous studies^{94 (link)}.

Buspirone hydrochloride was bought from Tocris (Cat. no. 0962), oxytocin was purchased from Sigma Aldrich (Cat. no. O3251) and risperidone was purchased from Tocris (Cat. no. 2865). Administration and concentration of the treatments were chosen according to previously published studies. Buspirone was applied by immersion in water containing 10 mg/L drug for 1 h (Bencan et al., 2009 (link)). oxytocin was applied at a concentration of 10 ng/kg by intraperitoneal injection 30 min before recording (Zimmermann et al., 2016 (link)). risperidone was applied by immersion in water containing 170 μg/L drug for 15 min (Idalencio et al., 2015 (link)). For buspirone: n = 8 WT, n = 8 reln^–/–. For oxytocin: n = 9 WT, n = 9 reln^–/–. For risperidone: n = 9 WT, n = 9 reln^–/–.