Propofol o glucuronide

Manufactured by LGC

Sourced in Canada

Propofol-O-glucuronide is a metabolite of the anesthetic drug propofol. It is used as a reference standard in analytical and research applications involving the detection and quantification of propofol in various matrices.

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Lab products found in correlation

D saccharic 1, by Merck Group (4 mentions) β estradiol 3 o glucuronide, by LGC (4 mentions) Alamethicin, by Merck Group (4 mentions) 4 lactone monohydrate, by Merck Group (2 mentions) Nicotinamide adenine dinucleotide phosphate nadph, by Merck Group (2 mentions) Cyp1a1, by Corning (2 mentions) Udpga, by Merck Group (2 mentions) 4 lactone, by Merck Group (2 mentions) Mgcl2, by Merck Group (2 mentions) Nadph, by Merck Group (2 mentions) Magnesium chloride mgcl2, by Merck Group (1 mentions) Uridine 5 diphospho glucuronosyltransferase udpga, by Merck Group (1 mentions) Uridine 5 diphosphoglucuronic acid udpga, by Merck Group (1 mentions) Ugt1a1, by Corning (1 mentions) Mini pig liver microsomes, by Corning (1 mentions) Mk571, by Merck Group (1 mentions) Dipyridamole dipy, by Merck Group (1 mentions) Pooled human liver microsomes, by Corning (1 mentions) Uridine diphosphate glucuronic acid, by Merck Group (1 mentions) Propofol, by Merck Group (1 mentions)

5 protocols using propofol o glucuronide

In Vitro Metabolic Profiling of Isobavachin

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Isobavachin (purity above 98%) was purchased from Shanghai Winherb Medical Technology Co., Ltd (Shanghai, China). Alamethicin, D-saccharic-1, 4-lactone monohydrate, magnesium chloride (MgCl₂), nicotinamide adenine dinucleotide phosphate (NADPH) and uridine 5′-diphospho-glucuronosyltransferase (UDPGA) were all obtained from Sigma-Aldrich (St. Louis, MO, USA). Specific substrates and their corresponding metabolites including phenacetin, paracetamol, bupropion, hydroxybupropion, paclitaxel, 6α-hydroxy-paclitaxel, tolbutamide, 4-hydroxytolbutamide, mephenytoin, 4-hydroxymephenytoin, chlorzoxazone, 6-hydroxychlorzoxazone, nifedipine, oxidized nifedipine, β-estradiol, propofol and zidovudine were acquired from Aladdin Chemicals (Shanghai, China). β-estradiol-3-O-glucuronide, propofol-O-glucuronide and AZT-N-glucuronide used in this study were from Toronto Research Chemicals (North York, ON, Canada).
Pooled HLM, individual HLM (iHLM), recombinant human CYP isozymes (rCYP1A2, 2B6, 2C8, 2C9, 2C19, 2E1, 3A4) and UGT isoforms (rUGT1A1, 1A9, 2B7) were all provided by Corning Biosciences (Corning, NY, USA). Mice liver microsomes (MLM) and mice intestine microsomes (MIM) were prepared in our laboratory using the differential centrifugation method as described previously.^{[16 (link)]} Other chemicals and reagents were of analytical grade or better.

Xing H., Yang J., Ren K., Qin Z., Wang P., Zhang X., Yao Z., Gonzalez F.J, & Yao X. (2020). Investigation on the metabolic characteristics of isobavachin in Psoralea corylifolia L. (Bu-gu-zhi) and its potential inhibition against human cytochrome P450s and UDP-glucuronosyltransferases. The Journal of pharmacy and pharmacology, 72(12), 1865-1878.

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In Vitro Metabolism Evaluation

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Bavachinin (purity over 98.0%) was purchased from Shanghai Winherb Medical Technology Co., Ltd (Shanghai, China). Magnesium chloride (MgCl₂), alamethicin, d-saccharic-1, 4-lactone monohydrate, uridine 5′-diphosphoglucuronic acid (UDPGA) and nicotinamide adenine dinucleotide phosphate (NADPH) were all obtained from Sigma-Aldrich (St. Louis, MO, USA). Bupropion, β-estradiol, chlorzoxazone, hydroxy-bupropion, 4-hydroxymephenytoin, 4-hydroxytolbutamide, mephenytoin, 6α-hydroxy-paclitaxel, 6-hydroxychlorzoxazone, nifedipine, oxidized nifedipine, paclitaxel, paracetamol, phenacetin, propofol, tolbutamide, and zidovudine were obtained from Aladdin Chemicals (Shanghai, China). β-Estradiol-3-O-glucuronide, propofol-O-glucuronide and zidovudine-N-glucuronide were purchased from Toronto Research Chemicals (North York, ON, Canada).
Pooled human liver microsomes (HLM, 50 donors), pooled human intestine microsomes (HIM, 20 donors), rats' liver microsomes (RLM), mice liver microsomes (MLM), dog's liver microsomes (DLM), mini-pig liver microsomes (MpLM), human CYP isozymes (CYP1A1, 1A2, 1B1, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4, 3A5), and human UGT isoforms (UGT1A1, 1A3, 1A4, 1A6, 1A7, 1A8, 1A9, 2B4, 2B7, 2B10, 2B15, 2B17) were all provided from Corning Biosciences (Corning, NY, USA). All other chemicals and reagents were used as received.

Li X., Xing H., Qin Z., Yang J., Wang P., Zhang X., Yao Z, & Yao X. (2020). Potential metabolism determinants and drug–drug interactions of a natural flavanone bavachinin. RSC Advances, 10(58), 35141-35152.

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Corylifol A Metabolism Assay

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Corylifol A (purity >98%) was purchased from Shanghai Winherb Medical Technology Co., Ltd (Shanghai, China). Pooled human liver microsomes (HLM), nine individual human liver microsomes (iHLM), mice liver microsomes (MLM), monkeys liver microsomes (MkLM), rabbits liver microsomes (RaLM), rats liver microsomes (RLM), dogs liver microsomes (DLM), twelve recombinant human UGT isoforms (UGT1A1, 1A3, 1A4, 1A6, 1A7, 1A8, 1A9, 2B4, 2B7, 2B10, 2B15 and 2B17) and twelve expressed human CYP enzymes (CYP1A1, 1A2, 1B1, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4 and 3A5) were all obtained from Corning Biosciences (Corning, NY, USA). The anti-BCRP (TA322704), anti-MRP1 (TA309559), anti-MRP3 (TA314800) and anti-MRP4 (TA327332) antibodies were purchased from OriGene Technologies (Rockville, MD). UDPGA, NADPH, MgCl₂, alamethicin and D-saccharic-1, 4-lactone, dipyridamole (DIPY) and MK571 were all provided from Sigma-Aldrich (St Louis, MO, USA). Paclitaxel, 6-hydroxy-paclitaxel, β-estradiol and propofol were supplied from Aladdin Chemicals (Shanghai, China). Propofol-O-glucuronide and β-estradiol-3-O-glucuronide were obtained from Toronto Research Chemicals (Ontario, Canada). Besides, other chemicals and solvents were of analytical grade or better.

Li Y., Xu J., Xu C., Qin Z., Li S., Hu L., Yao Z., Gonzalez F.J, & Yao X. (2020). Metabolism and disposition of corylifol A from Psoralea corylifolia: metabolite mapping, isozyme contribution, species differences and identification of efflux transporters for corylifol A-O-glucuronide in HeLa1A1 cells. Xenobiotica; the fate of foreign compounds in biological systems, 50(8), 997-1008.

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Neobavaisoflavone Metabolic Profiling

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Neobavaisoflavone (purity > 98%) was purchased from Shanghai Winherb Medical Technology Co., Ltd. (Shanghai, China). Alamethicin, D-saccharic-1, 4-lactone, MgCl₂, NADPH, and UDPGA were from Sigma-Aldrich (St. Louis, MO, USA). Human liver microsomes (HLM) and human intestine microsomes (HIM) were pooled from 20 and 50 mixed gender donors, respectively, and were both purchased form Corning Biosciences (Corning, NY, USA). Dogs liver microsomes (DLM), expressed human CYP enzymes (CYP1A1, 1A2, 1B1, 2A6, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4, and 3A5), individual pooled human liver microsomes (iHLM), mice liver microsomes (MLM), monkeys liver microsomes (MkLM), rabbits liver microsomes (RaLM), rats liver microsomes (RLM), and recombinant UGT enzymes (UGT1A1, 1A3, 1A4, 1A6, 1A7, 1A8, 1A9, 1A10, 2B4, 2B7, 2B10, 2B15, and 2B17) were obtained from Corning Biosciences. Phenacetin, paracetamol, paclitaxel, 6α-hydroxy-paclitaxel, β-estradiol, and propofol and were purchased from Aladdin Chemicals (Shanghai, China). Propofol-O-glucuronide and β-estradiol-3-O-glucuronide were obtained from Toronto Research Chemicals (Ontario, Canada). All other chemicals and reagents were of analytical grade or the highest grade commercially available.

Xu J., Li M., Yao Z., Zhang Y., Li S., Hu L., Qin Z., Gonzalez F.J, & Yao X. (2018). In vitrometabolic mapping of neobavaisoflavone in human cytochromes P450 and UDP-glucuronosyltransferase enzymes by ultra high-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Journal of pharmaceutical and biomedical analysis, 158, 351-360.

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Establishment and Characterization of UGT1A9-Overexpressing HeLa Cells

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Human UGT1A9-overexpressing HeLa cells (HeLa1A9 cells) were established as described previously (Jiang et al., 2011 (link)), and were provided by Prof. Baojian Wu who works in Jinan University in Guangzhou of China. Alamethicin, D-saccharic-1,4-lactone, magnesium chloride (MgCl₂), MK571, Ko143, propofol, and uridine diphosphate glucuronic acid (UDPGA) were purchased from Sigma-Aldrich (St Louis, MO, United States), whereas propofol-O-glucuronide from Toronto Research Chemicals (North York, ON, Canada). Fraxetin was provided by Guangzhou Fans Biotechnology Co., Ltd., (Guangzhou, China), and human UGT1A9 was purchased from Corning Biosciences (New York, NY, United States). UGT1A9 antibody was purchased from BD Biosciences (Woburn, MA, United States). The anti-BCRP, anti-MRP1, anti-MRP2, anti-MRP3, and anti-MRP4 antibodies were purchased from OriGene Technologies (Rockville, MD, United States). All other chemicals and reagents (analytical grade or better) were commercially available.

Qin Z., Zhang B., Yang J., Li S., Xu J., Yao Z., Zhang X., Gonzalez F.J, & Yao X. (2019). The Efflux Mechanism of Fraxetin-O-Glucuronides in UGT1A9-Transfected HeLa Cells: Identification of Multidrug Resistance-Associated Proteins 3 and 4 (MRP3/4) as the Important Contributors. Frontiers in Pharmacology, 10, 496.

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