Cells were collected and washed briefly with a 0.1 M sodium cacodylate buffer prior to fixation with 2.5% glutaraldehyde in 0.1 M sodium cacodylate overnight at 4°C. After being rinsed for 10 min in the same buffer, the cells were fixed at 4°C for 1 h with 1% OsO4 in 0.1 M sodium cacodylate. Following dehydration with a standard ethanol series and infiltration in epoxy resin at room temperature overnight, cells were transferred to beam capsules for polymerization in the oven (35°C for 6 h, 42°C for 18 h and 60°C for 48 h). The capsules were separated from the polymerized resin with a razor blade, and embedded cells in hardened blocks were viewed with an optical microscope so that the appropriate area was chosen for ultrathin sectioning (75 nm thick). Subsequently, ultrathin sections were obtained using an ultramicrotome with a diamond knife. Heavy metal staining was performed at room temperature for 30 min with 4% uranyl acetate and lead citrate, and the samples were examined under an electron microscope (H-7800; Hitachi, Ltd.).
H 7800
Manufactured by Hitachi
Sourced in Japan
The H-7800 is a transmission electron microscope (TEM) manufactured by Hitachi. It is designed to provide high-resolution imaging and analysis of materials at the nanoscale level. The H-7800 is capable of operating at accelerating voltages up to 200 kV, enabling the observation of a wide range of samples, from biological specimens to advanced materials.
Automatically generated - may contain errors
Lab products found in correlation
220 twin, by Particle Metrix (2 mentions)
Tem fixative, by Wuhan Servicebio Technology (1 mentions)
Electron microscopy fixative, by Wuhan Servicebio Technology (1 mentions)
0.22 μm filter, by Merck Group (1 mentions)
Bca protein assay kit, by Vazyme (1 mentions)
Su8220, by Hitachi (1 mentions)
Sz 100, by Horiba (1 mentions)
Model v 700, by Jasco (1 mentions)
Lowicryl hm20 resin, by Electron Microscopy Sciences (1 mentions)
D35 14 1.5gi, by Cellvis (1 mentions)
Em uc7, by Leica (1 mentions)
Morada g3, by EMSIS (1 mentions)
Litesizer 500, by Anton Paar (1 mentions)
P1127, by Solarbio (1 mentions)
Epon 812, by Merck Group (1 mentions)
Exosome isolation kit, by Umibio (1 mentions)
Bca protein assay kit, by CWBIO (1 mentions)
Ab236630, by Abcam (1 mentions)
Quanta 250, by Thermo Fisher Scientific (1 mentions)
Cary 100, by Agilent Technologies (1 mentions)
29 protocols using h 7800
1
Electron Microscopy Sample Preparation Protocol
2
Transmission Electron Microscopy of HDL
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Transmission electron microscopy (TEM, Hitachi H-7800; Ibaraki, Japan) was performed at the Raydel Research Institute (Daegu, Republic of Korea) at an acceleration voltage of 80 kV. HDL was negatively stained with 1% sodium phosphotungstate (PTA; pH 7.4) with a final apolipoprotein concentration of 0.3 mg/mL in TBS. A volume of 5 μL of the HDL suspension was blotted with filter paper and replaced immediately with a 5 μL droplet of 1% PTA. After a few seconds, the stained HDL fraction was blotted onto a Formvar carbon-coated 300 mesh copper grid and air-dried. The shape and size of the HDL particles were determined by TEM at 40,000× magnification, according to a previous report [43 (link),46 (link)].
3
Transmission Electron Microscopy of Colon Tissue
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The colon tissues were fixed with fresh TEM fixative (Servicebio, Wuhan, China) at 4 °C and washed with PBS (pH 7.4). Tissues were dehydrated at room temperature using ethanol and embedded in resin. Resin blocks were sectioned into 60–80 nm thin and stained with uranium acetate and lead citrate for 8 min. Images were taken under a transmission electron microscope (H7800; Hitachi Ltd., Japan).
4
Ultrastructural Analysis of Hippocampal CA1 Region
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According to our previously published method [3 (link)], the CA1 subregion of the hippocampus was dissected and cut into 1 mm sections as soon as possible after removing the brain. Then, after fixation in electron microscopy fixative (Servicebio, China) overnight at 4 °C, the sections were washed with 0.1 M PBS and postfixed with OsO4 for 2 h at room temperature. Next, the blocks were dehydrated by graded ethanol, embedded in resin, and eventually cut into ultrathin slices (60-80 nm), which were stained with uranyl acetate and lead citrate. The slices were then analysed using a transmission electron microscope (H-7800, Hitachi, Japan).
5
Isolation and Characterization of hucMSC-sEVs
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The hucMSC-sEVs were isolated and purified as previously described [24 (link), 25 (link)]. The conditioned medium of P3-6 hucMSCs with good growth condition was collected. Cell supernatants were centrifuged to remove cell debris and organelles. Finally, the exosome pellets were resuspended in PBS and then passed through a 0.22-μm filter (Millipore, USA) and stored at − 80 °C. The protein content of the hucMSC-sEVs was determined by using a BCA protein assay kit (Vazyme, Nanjing, China). The morphology of the hucMSC-sEVs was observed using transmission electron microscopy (TEM; H-7800, Hitachi, Japan). The particle size, concentration, and zeta potential of the hucMSC-sEVs were analyzed by Nanoparticle tracking analyzer (NTA) (Germany, Particle Metrix, 220-Twin). The positive markers of hucMSC-sEVs, such as CD9, CD63, CD81, TSG101, Alix, and HSP70, as well as the negative control Calnexin, were determined by western blotting.
6
Transmission Electron Microscopy of OMVs
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A transmission electron microscope (Hitachi H-7800, Japan) was used to examine the morphology and size of bacterial OMVs. Briefly, 20 μl of exosomal suspension, viral suspension, nanomaterial suspension, or other suspensions was dropped onto the copper grid with carbon film for 3–5 min. Then the excess liquid was absorbed using filter paper. Subsequently, 2% phosphotungstic acid was dropped on the copper grid to stain for 1–2 min, then the excess liquid was absorbed using filter paper, and the copper grid was dried at room temperature. The cuprum grids were examined under TEM, and images were acquired.
7
Ultrastructural Changes in C. albicans Mutants
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The membrane damage caused by the deletion of the ERG251 gene and the ERG3 gene or by FLC plus CZ66 were imaged by transmission electron microscopy. Briefly, 100 μL of C. albicans cultured in YPD medium overnight were added to 10 mL fresh YPD medium with or without indicated FLC or CZ66 and incubated at 30°C for 12 h with shaking at 200 rpm. After centrifugation, cell pellets were fixed with 2.5% glutaraldehyde at 4°C for 24 h. After that, the pellets were dropped onto the copper grid with carbon film for 3 to 5 min; 2% phosphotungstic acid was dropped on the copper grid to stain for 1 to 2 min; filter paper was used to absorb excess liquid and dry at room temperature. The copper grids were observed under a Hitachi H-7800 transmission electron microscope and images were obtained. The ultrastructures of strain SN152, the ERG251 gene null mutant, and the ERG3 gene null mutant were compared to assess the effects of compounds.
8
Characterization of CuO2, mPDA, and mPDA@CuO2 Nanostructures
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The morphology of CuO2, mPDA NPs, and mPDA@CuO2 NRs was characterized by transmission electron microscopy (TEM, H-7800, Hitachi), and the elemental composition of materials were characterized by energy dispersive spectroscopy on the scanning electron microscope (EDS-SEM, SU8220, Hitachi). The zeta potential and dispersion stability of materials were determined by dynamic light scattering (DLS, SZ-100, HORIBA, Japan). The catalyzed activity of mPDA@CuO2 NRs was analyzed by UV/VIS/NIR spectroscopy (MODEL V-700, JASCO, Japan).
9
Membrane Fraction TEM Imaging Protocol
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Five μL of membrane fraction sample was placed on an effluved carbon formvar grid and allowed to rest for 20 min before blotting with filter paper. Samples were negative stained with commercial solution (Uranyless EMS, USA) during 3 min before blotting and air drying. Transmission electron microscopy (TEM) images were taken with a Hitachi H7800 at an acceleration voltage of 100 kV and an AMT camera.
10
Immunolabeling of High-Pressure Frozen Samples
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For immunolabeling of high-pressure frozen samples, the freeze substitution medium consisted of anhydrous acetone containing 0.2% uranyl acetate in the AFS unit as described above at − 90 °C for 4 days, followed by slow warming to − 50 °C over a period of 2 days. After rinsing in several acetone washes, samples were infiltrated in Lowicryl® HM20 resin (MonoStep HM20 resin, Electron Microscopy Sciences, Hatfield, United States) at − 50 °C, polymerized under UV light, and subsequently sectioned. Ultrathin sections (82 nm) were cut as above and were collected onto carbon-collodion-coated 200-mesh grids. A solution of caveolin antibody diluted at 1/75 and of a goat anti-mouse conjugated with 5-nm colloidal gold diluted 1/25 (secondary antibody) were successively applied prior to TEM observations. These observations were carried out using an electron microscope (HITACHI H7800, Japan) as described above.
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